Proliferation Associated Protein 2G4 antibody | OTI1D3

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Mouse anti Proliferation Associated Protein 2G4

Proliferation associated protein 2G4 antibody recognizes proliferation-associated protein 2G4, also known as ErbB-3 binding protein 1, ErbB3-binding protein Ebp1 or cell cycle protein p38-2G4 homolog. This antibody has been extensively validated for western blotting using whole cell lysates.

Proliferation Associated Protein 2G4 Antibody with Control Lysate

Proliferation associated protein 2G4 antibody recognizes proliferation-associated protein 2G4, also known as ErbB-3 binding protein 1, ErbB3-binding protein Ebp1 or cell cycle protein p38-2G4 homolog. This antibody has been extensively validated for western blotting using whole cell lysates.

Product Type
PrecisionAb™ Monoclonal
Clone
OTI1D3
Isotype
IgG1
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
VMA00111 WB 100 µl
VMA00111KT WB 2 Westerns
With Lysate 400µg
Mouse anti Human proliferation associated protein 2G4 antibody recognizes proliferation associated protein 2G4, also known as ErbB-3 binding protein 1, ErbB3-binding protein Ebp1, cell cycle protein p38-2G4 homolog and erbB3-binding protein 1.

Proliferation associated protein 2G4, encoded by the PA2G4 gene, is an RNA-binding protein that is involved in growth regulation. This protein is present in pre-ribosomal ribonucleoprotein complexes and may be involved in ribosome assembly and the regulation of intermediate and late steps of rRNA processing. This protein can interact with the cytoplasmic domain of the ErbB3 receptor and may contribute to transducing growth regulatory signals. It is also a transcriptional co-repressor of androgen receptor-regulated genes and other cell cycle regulatory genes through its interactions with histone deacetylases. PA2G4 has been implicated in growth inhibition and the induction of differentiation of human cancer cells. Six pseudogenes, located on chromosomes 3, 6, 9, 18, 20 and X, have been identified (provided by RefSeq, Jul 2008).

Mouse anti Human proliferation associated protein 2G4 antibody detects a band of 44 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Product Form
Purified IgG - liquid
Preparation
Mouse monoclonal antibody purified by affinity chromatography from ascites.
Preparation
20μl Mouse monoclonal antibody purified by affinity chromatography from ascites.
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
50% Glycerol
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
50% Glycerol
Immunogen
Full length recombinant human proliferation associated protein 2G4 (NP_006182) produced in HEK293T cells

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Storage
Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
Shelf Life
As supplied, 12 months from date of despatch.
Shelf Life
As supplied, 12 months from date of despatch.

More Information

UniProt
Q9UQ80 Related reagents
Entrez Gene
PA2G4 Related reagents
GO Terms
GO:0003677 DNA binding
GO:0003700 sequence-specific DNA binding transcription factor activity
GO:0003723 RNA binding
GO:0030529 ribonucleoprotein complex
GO:0005737 cytoplasm
GO:0005730 nucleolus
GO:0006364 rRNA processing
GO:0006417 regulation of translation
GO:0007050 cell cycle arrest
GO:0008283 cell proliferation
GO:0031625 ubiquitin protein ligase binding
GO:0045892 negative regulation of transcription, DNA-dependent
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Regulatory
For research purposes only

Applications of Proliferation Associated Protein 2G4 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000
Western Blotting 1/1000

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
Western Blotting
Anti proliferation associated protein 2G4 detects a band of approximately 44 kDa in HeLa cell lysates.
Western Blotting
Anti proliferation associated protein 2G4 detects a band of approximately 44 kDa in HeLa cell lysates.
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Goat anti Mouse IgG (H/L):HRP STAR207P 2 ml WB
Goat anti Mouse IgG (H/L):HRP STAR207P 2 ml WB