Proliferation Associated Protein 2G4 antibody | OTI1D3

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Anti Proliferation Associated Protein 2G4 Antibody, clone OTI1D3 (PrecisionAb™ Monoclonal Antibody) gallery image 1 — Western blot analysis of whole cell lysates probed with proliferation associated protein 2G4 antibody followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000, STAR207P) and visualized on the ChemiDoc™ MP with 28 second exposure. Arrow points to proliferation associated protein 2G4 (molecular weight 44 kDa)

Mouse anti Proliferation Associated Protein 2G4

Proliferation associated protein 2G4 antibody recognizes proliferation-associated protein 2G4, also known as ErbB-3 binding protein 1, ErbB3-binding protein Ebp1 or cell cycle protein p38-2G4 homolog. This antibody has been extensively validated for western blotting using whole cell lysates.

Proliferation Associated Protein 2G4 Antibody with Control Lysate

Product Type: PrecisionAb™ Monoclonal
Clone: OTI1D3
Isotype: IgG1

Product Code	Applications	Pack Size
VMA00111	WB	100 µl
VMA00111KT	WB	2 Westerns
With Lysate		400µg

Summary
Secondary Antibodies

Mouse anti Human proliferation associated protein 2G4 antibody recognizes proliferation associated protein 2G4, also known as ErbB-3 binding protein 1, ErbB3-binding protein Ebp1, cell cycle protein p38-2G4 homolog and erbB3-binding protein 1.

Proliferation associated protein 2G4, encoded by the PA2G4 gene, is an RNA-binding protein that is involved in growth regulation. This protein is present in pre-ribosomal ribonucleoprotein complexes and may be involved in ribosome assembly and the regulation of intermediate and late steps of rRNA processing. This protein can interact with the cytoplasmic domain of the ErbB3 receptor and may contribute to transducing growth regulatory signals. It is also a transcriptional co-repressor of androgen receptor-regulated genes and other cell cycle regulatory genes through its interactions with histone deacetylases. PA2G4 has been implicated in growth inhibition and the induction of differentiation of human cancer cells. Six pseudogenes, located on chromosomes 3, 6, 9, 18, 20 and X, have been identified (provided by RefSeq, Jul 2008).

Mouse anti Human proliferation associated protein 2G4 antibody detects a band of 44 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species

Human

Species Cross-Reactivity

Target Species	Cross Reactivity
Mouse
Rat

N.B. Antibody reactivity and working conditions may vary between species.

Product Form

Purified IgG - liquid

Product Form

Purified IgG - liquid

Preparation

Mouse monoclonal antibody purified by affinity chromatography from ascites.

Preparation

20μl Mouse monoclonal antibody purified by affinity chromatography from ascites.

Buffer Solution

Phosphate buffered saline

Buffer Solution

Phosphate buffered saline

Preservative Stabilisers

0.09% Sodium Azide (NaN₃)
1% Bovine Serum Albumin
50% Glycerol

Preservative Stabilisers

0.09% Sodium Azide (NaN₃)
1% Bovine Serum Albumin
50% Glycerol

Immunogen

Full length recombinant human proliferation associated protein 2G4 (NP_006182) produced in HEK293T cells

Storage Information

Storage: Store undiluted at -20^oC, avoiding repeated freeze thaw cycles.
Storage: Antibody: Store undiluted at -20^oC, avoiding repeated freeze thaw cycles.

Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
Shelf Life: As supplied, 12 months from date of despatch.
Shelf Life: As supplied, 12 months from date of despatch.

More Information

UniProt: Q9UQ80 Related reagents
Entrez Gene: PA2G4 Related reagents
GO Terms: GO:0003677 DNA binding; GO:0003700 sequence-specific DNA binding transcription factor activity; GO:0003723 RNA binding; GO:0030529 ribonucleoprotein complex; GO:0005737 cytoplasm; GO:0005730 nucleolus; GO:0006364 rRNA processing; GO:0006417 regulation of translation; GO:0007050 cell cycle arrest; GO:0008283 cell proliferation; GO:0031625 ubiquitin protein ligase binding; GO:0045892 negative regulation of transcription, DNA-dependent
Acknowledgements: PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Acknowledgements: PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Regulatory: For research purposes only

Applications of Proliferation Associated Protein 2G4 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Western Blotting		1/1000
Western Blotting		1/1000

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

Western Blotting: Anti proliferation associated protein 2G4 detects a band of approximately 44 kDa in HeLa cell lysates.
Western Blotting: Anti proliferation associated protein 2G4 detects a band of approximately 44 kDa in HeLa cell lysates.
Instructions For Use: Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
Instructions For Use: Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.

Secondary Antibodies Available

Description	Product Code	Pack Size	Applications	List Price	Quantity
Goat anti Mouse IgG (H/L):HRP	STAR207P	2 ml	WB
Goat anti Mouse IgG (H/L):HRP	STAR207P	2 ml	WB

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