PRKAR1A antibody | OTI6C7

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Anti PRKAR1A Antibody, clone OTI6C7 (PrecisionAb Monoclonal Antibody) thumbnail image 1

Anti PRKAR1A Antibody, clone OTI6C7 (PrecisionAb Monoclonal Antibody) thumbnail image 2

Anti PRKAR1A Antibody, clone OTI6C7 (PrecisionAb Monoclonal Antibody) thumbnail image 3

Anti PRKAR1A Antibody, clone OTI6C7 (PrecisionAb Monoclonal Antibody) gallery image 1 — Western blot analysis of whole cell lysates probed with PRKAR1A antibody followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000, STAR207P) and visualized on the ChemiDoc MP with 10 second exposure. Arrow points to PRKAR1A (molecular weight 43 kDa)

Anti PRKAR1A Antibody, clone OTI6C7 (PrecisionAb Monoclonal Antibody) gallery image 2 — Western blot analysis of whole cell lysates prepared from HepG2 separately transfected with two unique siRNA duplexes against PRKAR1A (1 & 2) or a scrambled siRNA (-) probed with Mouse anti PRKAR1A antibody (**VMA00270**) followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000, 1705047) and hFAB rhodamine anti-tubulin primary antibody (12004166) and visualized on the ChemiDoc MP with a 10 second (chemiluminescent channel) and 60 second exposure

Anti PRKAR1A Antibody, clone OTI6C7 (PrecisionAb Monoclonal Antibody) gallery image 3 — Normalized signal intensity of PRKAR1A from whole cell lysates prepared from HepG2 separately transfected with two unique siRNA duplexes against PRKAR1A (1 & 2) or a scrambled siRNA (-). Signal intensity was normalized to the tubulin signal.

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Mouse anti PRKAR1A

Product Type: PrecisionAb Monoclonal
Clone: OTI6C7
Isotype: IgG1
Format: Purified
Specificity: PRKAR1A

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VMA00270 Datasheet Datasheet SDS SDS	WB	100 µl		Log in	Get Quote
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Mouse anti Human PRKAR1A antibody recognizes the cAMP-dependent protein kinase type I-alpha regulatory subunit, also known as Carney complex type 1, cAMP-dependent protein kinase regulatory subunit RIalpha, cAMP-dependent protein kinase type I-alpha regulatory chain, cAMP-dependent protein kinase type I-alpha regulatory subunit, protein kinase A type 1a regulatory subunit and tissue-specific extinguisher 1.

cAMP is a signaling molecule important for a variety of cellular functions. cAMP exerts its effects by activating the cAMP-dependent protein kinase, which transduces the signal through phosphorylation of different target proteins. The inactive kinase holoenzyme is a tetramer composed of two regulatory and two catalytic subunits. cAMP causes the dissociation of the inactive holoenzyme into a dimer of regulatory subunits bound to four cAMP and two free monomeric catalytic subunits. Four different regulatory subunits and three catalytic subunits have been identified in humans. PRKAR1A encodes one of the regulatory subunits. This protein was found to be a tissue-specific extinguisher that down-regulates the expression of seven liver genes in hepatoma x fibroblast hybrids. Mutations in PRKAR1A gene cause Carney complex (CNC). PRKAR1A can fuse to the RET protooncogene by gene rearrangement and form the thyroid tumor-specific chimeric oncogene known as PTC2. A nonconventional nuclear localization sequence (NLS) has been found for this protein which suggests a role in DNA replication via the protein serving as a nuclear transport protein for the second subunit of the Replication Factor C (RFC40). Several alternatively spliced transcript variants encoding two different isoforms have been observed (provided by RefSeq, Jan 2013).

Mouse anti Human PRKAR1A antibody detects a band of 43 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Target Species: Human
Western Blotting: Anti PRKAR1A detects a band of approximately 43 kDa in Jurkat cell lysates.
Species Cross-Reactivity: Target Species Cross Reactivity

Mouse

Rat; N.B. Antibody reactivity and working conditions may vary between species.
Product Form: Purified IgG - liquid
Preparation: Mouse monoclonal antibody purified by affinity chromatography from ascites.
Buffer Solution: Phosphate buffered saline.
Preservative Stabilisers: 0.09% Sodium Azide (NaN₃)
1% Bovine Serum Albumin
50% Glycerol
Immunogen: Full-length protein expressed in 293T cell transfected with human PRKAR1A expression vector
Approx. Protein Concentrations: IgG concentration 0.5 mg/ml.
Regulatory: For research purposes only.
Guarantee: 12 months from date of despatch.
Acknowledgements: PrecisionAb is a trademark of Bio-Rad Laboratories.

Target Species	Cross Reactivity
Mouse
Rat

Store undiluted at -20°C, avoiding repeated freeze thaw cycles.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Western Blotting		1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Description	Product Code	Applications	Pack Size	Your Price
Goat anti Mouse IgG (H/L):HRP	STAR207P	WB	2 ml	Log in	Get Quote
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Description	Goat anti Mouse IgG (H/L):HRP

UniProt: P10644
Entrez Gene: PRKAR1A
GO Terms: GO:0005515 protein binding; GO:0001932 regulation of protein phosphorylation; GO:0007596 blood coagulation; GO:0005829 cytosol; GO:0035556 intracellular signal transduction; GO:0005952 cAMP-dependent protein kinase complex; GO:0006112 energy reserve metabolic process; GO:0006357 regulation of transcription from RNA polymerase II promoter; GO:0006833 water transport; View More GO Terms; GO:0007202 activation of phospholipase C activity; GO:0050796 regulation of insulin secretion; GO:0008603 cAMP-dependent protein kinase regulator activity; GO:0030552 cAMP binding; GO:0034199 activation of protein kinase A activity; GO:0048011 nerve growth factor receptor signaling pathway; GO:0055085 transmembrane transport; GO:0071377 cellular response to glucagon stimulus