Poly (ADP-Ribose) Polymerase 1 antibody | A6.4.12
IP-MS Validated
Mouse anti Human poly (ADP-ribose) polymerase 1 antibody recognizes poly (ADP-ribose) polymerase 1 (PARP-1), a nuclear enzyme cleaved during apoptosis (Soldani et al. 2002).
PARP-1 is a caretaker enzyme involved in DNA damage repair (Langelier et al. 2013). It plays roles in diabetes pathophysiology (Andreone et al. 2012) and tumor proliferation (Rosado et al 2013 ). As well as protecting cells from genomic instability, PARP-1 is also involved in the development of both inflammatory and immune responses, and cell death by apoptosis and necrosis (Erdélyi et al. 2005).
PARP-1 represents a promising target for new developments in therapeutic treatment of immune mediated diseases (Rosado et al. 2013 ) and has considerable potential for delivering selective tumor cell killing while sparing normal cells (Pinton et al. 2013).
Mouse anti Human poly (ADP-ribose) polymerase 1 antibody recognizes PARP-1 as a single band of ~116 kDa in western blotting in multiple cell line lysates including myeloid, lymphoid, neuronal, adenocarcinoma, sarcoma and embryonic kidney.
PARP-1 is a caretaker enzyme involved in DNA damage repair (Langelier et al. 2013). It plays roles in diabetes pathophysiology (Andreone et al. 2012) and tumor proliferation (
PARP-1 represents a promising target for new developments in therapeutic treatment of immune mediated diseases (
Mouse anti Human poly (ADP-ribose) polymerase 1 antibody recognizes PARP-1 as a single band of ~116 kDa in western blotting in multiple cell line lysates including myeloid, lymphoid, neuronal, adenocarcinoma, sarcoma and embryonic kidney.
Product Details
- Target Species
- Human
- Western Blotting
- Anti Poly (ADP-Ribose) Polymerase 1 antibody detects a band of approximately 116 kDa in HEK293 cell lysates.
- Species Cross-Reactivity
-
Target Species Cross Reactivity Mouse Rat - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Preparation
- Mouse monoclonal antibody purified by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Immunogen
- Human PARP-1
- Approx. Protein Concentrations
- IgG concentration 0.5 mg/ml
Storage Information
- Storage
- Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
- Guarantee
- 12 months from date of despatch
More Information
- UniProt
- P09874
- Entrez Gene
- PARP1
- GO Terms
- GO:0003677 DNA binding
- GO:0003950 NAD+ ADP-ribosyltransferase activity
- GO:0005635 nuclear envelope
- GO:0005667 transcription factor complex
- GO:0005730 nucleolus
- GO:0006366 transcription from RNA polymerase II promoter
- GO:0006471 protein ADP-ribosylation
- GO:0008134 transcription factor binding
- GO:0008270 zinc ion binding
- GO:0042802 identical protein binding
- GO:0032869 cellular response to insulin stimulus
- GO:0045449 regulation of transcription
- GO:0047485 protein N-terminus binding
- Acknowledgements
- PrecisionAb is a trademark of Bio-Rad Laboratories.
- Regulatory
- For research purposes only
Applications of Poly (ADP-Ribose) Polymerase 1 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Immunoprecipitation | |||
| Western Blotting | 1/1000 |
The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.
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Secondary Antibodies Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Goat anti Mouse IgG (H/L):HRP | STAR207P | WB | 2 ml |
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