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Poly (ADP-Ribose) Polymerase 1 antibody | A6.4.12

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Anti Poly (ADP-Ribose) Polymerase 1 Antibody, clone A6.4.12 (PrecisionAb Monoclonal Antibody) thumbnail image 1 Anti Poly (ADP-Ribose) Polymerase 1 Antibody, clone A6.4.12 (PrecisionAb Monoclonal Antibody) thumbnail image 2
Anti Poly (ADP-Ribose) Polymerase 1 Antibody, clone A6.4.12 (PrecisionAb Monoclonal Antibody) gallery image 1
Western blot analysis of whole cell lysates probed with poly (ADP-ribose) polymerase 1 antibody (VMA00016) followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000, STAR207P) and visualized on the ChemiDoc MP with 4 second exposure. Arrow points to poly (ADP-ribose) polymerase 1 (molecular weight 116 kDa)
Anti Poly (ADP-Ribose) Polymerase 1 Antibody, clone A6.4.12 (PrecisionAb Monoclonal Antibody) gallery image 2
Immunoprecipitation of Poly (ADP-Ribose) Polymerase 1 from HEK293 whole cell lysate. The Input lane is 10% input; the Poly (ADP-Ribose) Polymerase 1-IP lane is the immunoprecipitation with the Poly (ADP-Ribose) Polymerase 1 antibody (2.5 µg, VMA00016); the IgG-IP lane is the immunoprecipitation with Mouse monoclonal IgG (2.5 µg, MCA928). Western blot analysis was performed using Poly (ADP-Ribose) Polymerase 1 antibody (1/1000, VMA00016) followed by detection with HRP conjugated Goat anti Mouse IgG (1/10000, 1705047) and visualized on the ChemiDoc MP. Arrow points to Poly (ADP-Ribose) Polymerase 1.

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WB IP

Mouse anti Poly (ADP-Ribose) Polymerase 1

Product Type
PrecisionAb Monoclonal
Clone
A6.4.12
Isotype
IgG1
Format
Purified
Specificity
Poly (ADP-Ribose) Polymerase 1

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Product Code Applications Pack Size List Price Your Price Qty
VMA00016
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
IP WB 100 µl loader
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loader

Mouse anti Human poly (ADP-ribose) polymerase 1 antibody recognizes poly (ADP-ribose) polymerase 1 (PARP-1), a nuclear enzyme cleaved during apoptosis (Soldani et al. 2002).

PARP-1 is a caretaker enzyme involved in DNA damage repair (Langelier et al. 2013). It plays roles in diabetes pathophysiology (Andreone et al. 2012) and tumor proliferation (Rosado et al 2013). As well as protecting cells from genomic instability, PARP-1 is also involved in the development of both inflammatory and immune responses, and cell death by apoptosis and necrosis (Erdélyi et al. 2005).

PARP-1 represents a promising target for new developments in therapeutic treatment of immune mediated diseases (Rosado et al. 2013) and has considerable potential for delivering selective tumor cell killing while sparing normal cells (Pinton et al. 2013).

Mouse anti Human poly (ADP-ribose) polymerase 1 antibody recognizes PARP-1 as a single band of ~116 kDa in western blotting in multiple cell line lysates including myeloid, lymphoid, neuronal, adenocarcinoma, sarcoma and embryonic kidney.		 PrecisionAb Antibodies

Target Species
Human
Western Blotting
Anti Poly (ADP-Ribose) Polymerase 1 antibody detects a band of approximately 116 kDa in HEK293 cell lysates.
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Mouse monoclonal antibody purified by affinity chromatography on Protein A from tissue culture supernatant.
Buffer Solution
Phosphate buffered saline.
Preservative Stabilisers
0.09% Sodium Azide (NaN3).
Immunogen
Human PARP-1
Approx. Protein Concentrations
IgG concentration 0.5 mg/ml
Regulatory
For research purposes only.
Guarantee
12 months from date of despatch.
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories.

Store undiluted at -20°C, avoiding repeated freeze thaw cycles.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunoprecipitation
Western Blotting 1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Description Product Code Applications Pack Size List Price Your Price Quantity
Goat anti Mouse IgG (H/L):HRP STAR207P WB 2 ml loader
List Price Your Price
loader
Description Goat anti Mouse IgG (H/L):HRP

UniProt
P09874
Entrez Gene
PARP1
GO Terms
GO:0003677 DNA binding
GO:0003950 NAD+ ADP-ribosyltransferase activity
GO:0005635 nuclear envelope
GO:0005667 transcription factor complex
GO:0005730 nucleolus
GO:0006366 transcription from RNA polymerase II promoter
GO:0006471 protein ADP-ribosylation
GO:0008134 transcription factor binding
GO:0008270 zinc ion binding
GO:0042802 identical protein binding
GO:0032869 cellular response to insulin stimulus
GO:0045449 regulation of transcription
GO:0047485 protein N-terminus binding

VMA00016

040714 157020 171128

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