POLD1 antibody | CD02/4G2

Mouse anti POLD1

Product Type
PrecisionAb Monoclonal
Clone
CD02/4G2
Isotype
IgG2b
Format
Purified
Specificity
POLD1

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VMA00977
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SDS Safety Datasheet SDS
IP WB 100 µl loader
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Mouse anti POLD1 antibody recognizes DNA polymerase delta catalytic subunit, also known as POLD. POLD is the catalytic subunit of the DNA polymerase delta complex, one of the two major polymerases in eukaryotes which function in DNA replication (Bulock et al. 2020). Accordingly, POLD1 is important for appropriate DNA replication and repair and maintenance of genome integrity. POLD1 is expressed differently throughout the cell cycle, with highest expression during late G1/S when DNA is replicated prior to mitosis (Nicolas et al. 2016). POLD1 expression declines with age, which appears to cause senescence by reducing DNA synthesis and repair of DNA damage (Gao et al. 2019). Abnormal POLD1 expression has been identified in many cancer types, and may play important roles in tumor development (Qin et al. 2018). High frequencies of POLD1 mutations have been identified in patients with endometrial cancer, skin cancer, colorectal cancer, esophagogastric cancer, bladder cancer, lung cancer and other cancer types (Wang et al. 2019). PrecisionAb Antibodies

Target Species
Human
Western Blotting
Mouse anti POLD1 detects a band of approximately 133 kDa in MOLT-4 cell lysates
Product Form
Purified IgG - Liquid
Preparation
Mouse monoclonal antibody affinity purified on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide
Immunogen
E. coli-derived recombinant protein of amino acids 1-140 of human POLD1
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunised BALB/c mice were fused with cells of the mouse SP2/0 myeloma cell line
Regulatory
For research purposes only
Guarantee
12 months from date of despatch
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories

Store undiluted at -20°C, avoiding repeated freeze thaw cycles

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunoprecipitation
Western Blotting 1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

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UniProt
P28340
Entrez Gene
POLD1
GO Terms
GO:0000084 S phase of mitotic cell cycle
GO:0000166 nucleotide binding
GO:0005515 protein binding
GO:0000722 telomere maintenance via recombination
GO:0000731 DNA synthesis involved in DNA repair
GO:0003677 DNA binding
GO:0003682 chromatin binding
GO:0003887 DNA-directed DNA polymerase activity
GO:0004527 exonuclease activity
GO:0046872 metal ion binding
GO:0005654 nucleoplasm
GO:0006271 DNA strand elongation involved in DNA replication
GO:0006283 transcription-coupled nucleotide-excision repair
GO:0006284 base-excision repair
GO:0006297 nucleotide-excision repair, DNA gap filling
GO:0009411 response to UV
GO:0032201 telomere maintenance via semi-conservative replication
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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