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PKA C-ALPHA antibody | AB01/1D9

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Anti PKA C-ALPHA Antibody, clone AB01/1D9 (PrecisionAb Monoclonal Antibody) thumbnail image 1 Anti PKA C-ALPHA Antibody, clone AB01/1D9 (PrecisionAb Monoclonal Antibody) thumbnail image 2 Anti PKA C-ALPHA Antibody, clone AB01/1D9 (PrecisionAb Monoclonal Antibody) thumbnail image 3
Anti PKA C-ALPHA Antibody, clone AB01/1D9 (PrecisionAb Monoclonal Antibody) gallery image 1
Western blot analysis of whole cell lysates probed with PRKACA antibody (1/1,000, VMA00679) followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000, STAR207P) and visualized on the ChemiDoc MP. Arrow points to PRKACA (molecular weight 38 kDa).
Anti PKA C-ALPHA Antibody, clone AB01/1D9 (PrecisionAb Monoclonal Antibody) gallery image 2
Immunoprecipitation of PRKACA from HeLa whole cell lysate. The Input lane is 10% input; the PRKACA-IP lane is the immunoprecipitation with the PRKACA antibody (2.5 µg, VMA00679); the IgG-IP lane is the immunoprecipitation with Mouse monoclonal IgG (2.5 µg, MCA928). Western blot analysis was performed using PRKACA antibody (1/1000, VMA00679) followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000, 1705047) and visualized on the ChemiDoc MP with 11 second exposure. Arrow points to PRKACA.
Anti PKA C-ALPHA Antibody, clone AB01/1D9 (PrecisionAb Monoclonal Antibody) gallery image 3
HeLa cells stained with Mouse anti PKA C-ALPHA antibody (VMA00679) at a 1/500 dilution followed by detection with an Alexa Fluor 488 conjugated Goat anti Mouse IgG (H/L) antibody (green) and PureBlu™ DAPI (1351303) (blue) as a nuclear counterstain.

Mouse anti PKA C-ALPHA

Product Type
PrecisionAb Monoclonal
Clone
AB01/1D9
Isotype
IgG1
Format
Purified
Specificity
PKA C-ALPHA

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Product Code Applications Pack Size List Price Your Price Qty
VMA00679
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IF IP WB 100 µl loader
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Mouse anti Human PKA C-alpha antibody recognizes PKA C-alpha, also known as cAMP-dependent protein kinase catalytic subunit alpha, PRKACA, PPNAD4 and protein kinase A catalytic subunit.

Encoded by PRKACA, the ubiquitously-expressed protein PKA C-alpha is the catalytic subunit C alpha of the cyclic-AMP (cAMP)-dependent protein kinase, otherwise known as PKA (Berthon et al. 2015). PKA is a serine/threonine kinase comprised of a regulatory subunit dimer and two catalytic subunits. Activation of PKA occurs when two cAMP molecules bind to each regulatory subunit, leading to release of the catalytic subunits. The catalytic subunits act as kinases propagate downstream signaling. Through this activity, PKA is responsible for phosphorylating a wide range of substrates, and is considered an essential regulator of many cell signaling events (Turnham and Scott 2016). Somatic PRKACA mutations have been identified in tumors of the adrenal cortex (Berthon et al. 2015). PRKACA may contribute to cancer progression, with extracellular PKA catalytic subunit found in the serum of patients with cancer, and increased PRKACA transcripts observed in breast cancer (Turnham and Scott 2016).		 PrecisionAb Antibodies

Target Species
Human
Western Blotting
Mouse anti PKA C-alpha detects a band of approximately 38 kDa in HeLa cell lysates.
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - Liquid
Preparation
Mouse monoclonal antibody affinity purified on Protein G from tissue culture supernatant.
Buffer Solution
Phosphate buffered saline.
Preservative Stabilisers
0.09% Sodium Azide.
Immunogen
E. coli-derived recombinant protein of amino acids 1-351 of human PKA C-alpha
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml.
Fusion Partners
Spleen cells from immunised BALB/c mice were fused with cells of the mouse SP2/0 myeloma cell line
Regulatory
For research purposes only.
Guarantee
12 months from date of despatch.
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories.

Store undiluted at -20°C, avoiding repeated freeze thaw cycles.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunofluorescence
Immunoprecipitation
Western Blotting 1/1000
The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Description Product Code Applications Pack Size List Price Your Price Quantity
Goat anti Mouse IgG (H/L):HRP STAR207P WB 2 ml loader
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Description Goat anti Mouse IgG (H/L):HRP

UniProt
P17612
Entrez Gene
PRKACA
GO Terms
GO:0000086 G2/M transition of mitotic cell cycle
GO:0007596 blood coagulation
GO:0005829 cytosol
GO:0004691 cAMP-dependent protein kinase activity
GO:0004862 cAMP-dependent protein kinase inhibitor activity
GO:0005524 ATP binding
GO:0005654 nucleoplasm
GO:0005952 cAMP-dependent protein kinase complex
GO:0006094 gluconeogenesis
GO:0006112 energy reserve metabolic process
GO:0006833 water transport
GO:0007202 activation of phospholipase C activity
GO:0007243 intracellular protein kinase cascade
GO:0050796 regulation of insulin secretion
GO:0019433 triglyceride catabolic process
GO:0019901 protein kinase binding
GO:0034199 activation of protein kinase A activity
GO:0048011 nerve growth factor receptor signaling pathway
GO:0055085 transmembrane transport
GO:0071377 cellular response to glucagon stimulus

VMA00679

100004127 100005793 64598215

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