PEA15 antibody | CD02/4E2
IP-MS Validated
Mouse anti PEA15
- Product Type
- PrecisionAb Monoclonal
- Clone
- CD02/4E2
- Isotype
- IgG1
- Format
- Purified
- Specificity
- PEA15
Antibody Quality and Performance Guarantee
| Mouse anti PEA15 antibody recognizes astrocytic phosphoprotein PEA-15, also known as PED. PEA15 is a multifunctional, non-enzymatic protein able to regulate a variety of signaling pathways and implicated in a range of biological processes. It can act as a tumor suppressor, regulating cell cycle progression by promoting the DNA damage-induced G2/M checkpoint and inhibiting RAS-mediated transformation. The function of PEA15 as a tumor suppressor can be attributed to its ability to restrict extracellular signal-related kinase (ERK) in the cytoplasm (Hindupur et al. 2014). Consistent with its role as a tumor suppressor, PEA15 is epigenetically silenced in many human tumors (Nagarajan et al. 2014). In ovarian carcinoma, high expression of PEA15 corresponds to increased patient survival. However. PEA15 also appears to have pro-tumorigenic properties. in glioblastoma, for example, PEA15 expression contributes to tumor survival (Gawecka et al. 2012). |
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- Target Species
- Human
- Western Blotting
- Mouse anti PEA15 detects a band of approximately 14 kDa in A172 cell lysates
- Species Cross-Reactivity
-
Target Species Cross Reactivity Mouse Rat - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - Liquid
- Preparation
- Mouse monoclonal antibody affinity purified on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide
- Immunogen
- E. coli-derived recombinant protein of amino acids 1-130 of human PEA15
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunised BALB/c mice were fused with cells of the mouse SP2/0 myeloma cell line
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
- Acknowledgements
- PrecisionAb is a trademark of Bio-Rad Laboratories
Store undiluted at -20°C, avoiding repeated freeze thaw cycles
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Immunoprecipitation |  |
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| Western Blotting | |
1/1000 |
The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Goat anti Mouse IgG (H/L):HRP | STAR207P | WB | 2 ml |
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| List Price | Your Price | ||||||
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| Description | Goat anti Mouse IgG (H/L):HRP | ||||||
- UniProt
- Q15121
- Entrez Gene
- PEA15
- GO Terms
- GO:0005515 protein binding
- GO:0006915 apoptosis
- GO:0005737 cytoplasm
- GO:0005875 microtubule associated complex
- GO:0006916 anti-apoptosis
- GO:0008643 carbohydrate transport
- GO:0046325 negative regulation of glucose import
VMA00976
100005824
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"
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