PEA15 antibody | CD02/4E2

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Mouse anti PEA15

Product Type
PrecisionAb Monoclonal
Clone
CD02/4E2
Isotype
IgG1
Format
Purified
Product Code Applications Pack Size List Price Quantity
VMA00976
Datasheet
WB
SDS
100 µl loader

Mouse anti PEA15 antibody recognizes astrocytic phosphoprotein PEA-15, also known as PED. PEA15 is a multifunctional, non-enzymatic protein able to regulate a variety of signaling pathways and implicated in a range of biological processes. It can act as a tumor suppressor, regulating cell cycle progression by promoting the DNA damage-induced G2/M checkpoint and inhibiting RAS-mediated transformation. The function of PEA15 as a tumor suppressor can be attributed to its ability to restrict extracellular signal-related kinase (ERK) in the cytoplasm (Hindupur et al. 2014). Consistent with its role as a tumor suppressor, PEA15 is epigenetically silenced in many human tumors (Nagarajan et al. 2014). In ovarian carcinoma, high expression of PEA15 corresponds to increased patient survival. However. PEA15 also appears to have pro-tumorigenic properties. in glioblastoma, for example, PEA15 expression contributes to tumor survival (Gawecka et al. 2012).

Product Details

Target Species
Human
Western Blotting
Mouse anti PEA15 detects a band of approximately 14 kDa in A172 cell lysates
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - Liquid
Preparation
Mouse monoclonal antibody affinity purified on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide
Immunogen
E. coli-derived recombinant protein of amino acids 1-130 of human PEA15
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunised BALB/c mice were fused with cells of the mouse SP2/0 myeloma cell line

Storage Information

Storage
Store undiluted at -20°C, avoiding repeated freeze thaw cycles
Guarantee
12 months from date of despatch

More Information

UniProt
Q15121
Entrez Gene
PEA15
GO Terms
GO:0005515 protein binding
GO:0006915 apoptosis
GO:0005737 cytoplasm
GO:0005875 microtubule associated complex
GO:0006916 anti-apoptosis
GO:0008643 carbohydrate transport
GO:0046325 negative regulation of glucose import
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories
Regulatory
For research purposes only

Applications of PEA15 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Mouse IgG (H/L):HRP STAR207P WB 2 ml

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