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PDPK1 antibody | EF01/2H4

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Anti PDPK1 Antibody, clone EF01/2H4 (PrecisionAb Monoclonal Antibody) thumbnail image 1 Anti PDPK1 Antibody, clone EF01/2H4 (PrecisionAb Monoclonal Antibody) thumbnail image 2
Anti PDPK1 Antibody, clone EF01/2H4 (PrecisionAb Monoclonal Antibody) gallery image 1
Western blot analysis of whole cell lysates probed with PDPK1 antibody (1/1,000, VMA00817) followed by detection with HRP conjugated Goat anti Mouse IgG (1/20,000, STAR207P) and visualized on the ChemiDoc MP. Arrow points to PDPK1 (molecular weight 56–69 kDa)
Anti PDPK1 Antibody, clone EF01/2H4 (PrecisionAb Monoclonal Antibody) gallery image 2
Immunoprecipitation of PDPK1 from Jurkat whole cell lysate. The Input lane is 10% input; the PDPK1-IP lane is the immunoprecipitation with the PDPK1 antibody (2.5 µg, VMA00817); the IgG-IP lane is the immunoprecipitation with Mouse monoclonal IgG (2.5 µg, MCA928 ). Western blot analysis was performed using PDPK1 antibody (1/1000, VMA00817) followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000, 1705047) and visualized on the ChemiDoc MP. Arrow points to PDPK1.

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WB IP

Mouse anti PDPK1

Product Type
PrecisionAb Monoclonal
Clone
EF01/2H4
Isotype
IgG1
Format
Purified
Specificity
PDPK1

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Product Code Applications Pack Size List Price Your Price Qty
VMA00817
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SDS Safety Datasheet SDS
IP WB 100 µl loader
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Mouse anti PDPK1 antibody recognizes 3-phosphoinositide-dependent protein kinase 1, otherwise known as PDK1.

PDPK1 is a serine/threonine kinase which belongs to the AGC family of protein kinases. The protein plays important roles in cell proliferation and survival, and is crucial for regulation of every step during cell migration (Di Blasio et al. 2017). PDPK1 affects a multitude of downstream processes including PI3K/Akt, Ras/MAPK and Myc signalling, which are often implicated in cancer. PDPK1 controls cancer cell invasion and dissemination, and consistent with this effect, its expression is dysregulated in many cancer types including lung and prostate cancer (Emmanouilidi and Falasca 2017). Targeting PDPK1 has shown to be effective in experimental models with alterations in downstream signalling pathways, and therefore, it has been suggested as a target for cancer therapy (Gagliardi et al. 2018), including the suggestion that PDPK1 inhibition could re-sensitize cancer cells to chemotherapeutic agents (Emmanouilidi and Falasca 2017).		 PrecisionAb Antibodies

Target Species
Human
Western Blotting
Mouse anti PDPK1 antibody detects a band of approximately 56–69 kDa in HEK293 cell lysates
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - Liquid
Preparation
Mouse monoclonal antibody affinity purified on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide
Immunogen
E. coli-derived recombinant protein of amino acids 411-556 of human PDPK1
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunised BALB/c mice were fused with cells of the mouse SP2/0 myeloma cell line
Regulatory
For research purposes only
Guarantee
12 months from date of despatch
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories

Store undiluted at -20°C, avoiding repeated freeze thaw cycles

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunoprecipitation
Western Blotting 1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Description Product Code Applications Pack Size List Price Your Price Quantity
Goat anti Mouse IgG (H/L):HRP STAR207P WB 2 ml
List Price Your Price
Description Goat anti Mouse IgG (H/L):HRP

UniProt
O15530
Entrez Gene
PDPK1
GO Terms
GO:0005886 plasma membrane
GO:0005515 protein binding
GO:0030036 actin cytoskeleton organization
GO:0005829 cytosol
GO:0004676 3-phosphoinositide-dependent protein kinase activity
GO:0005524 ATP binding
GO:0005654 nucleoplasm
GO:0006469 negative regulation of protein kinase activity
GO:0007268 synaptic transmission
GO:0008286 insulin receptor signaling pathway
GO:0018107 peptidyl-threonine phosphorylation
GO:0048015 phosphatidylinositol-mediated signaling
GO:0030168 platelet activation
GO:0031295 T cell costimulation
GO:0032148 activation of protein kinase B activity
GO:0048011 nerve growth factor receptor signaling pathway
GO:0050852 T cell receptor signaling pathway
GO:0090004 positive regulation of establishment of protein localization in plasma membrane

VMA00817

100004835

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