PDIA6 antibody | CD02/1D11

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Mouse anti PDIA6

Product Type
PrecisionAb Monoclonal
Clone
CD02/1D11
Isotype
IgG2b
Format
Purified
Product Code Applications Pack Size List Price Quantity
VMA00952
Datasheet
WB
SDS
100 µl loader

Mouse anti PDIA6 antibody recognizes protein disulfide-isomerase A6, also known as ER protein 5, TXNDC7. PDIA6 belongs to the protein disulfide isomerase (PDI) family. It functions as an oxidoreductase to catalyze the formation of disulfide bonds, and as a chaperone to assist with protein folding (Bai et al. 2019). PDIA6 is able to promote the proliferation and growth of many types of human cancer cell and plays important roles in tumor immunoevasion and proliferation (Gao et al. 2016). Overexpression of PDIA6 has been identified in patients with lung adenocarcinoma and correlates with clinical status (Tufo et al. 2014). PDIA6 also appears to have metabolic roles, having found to be essential for glucose-stimulated insulin secretion. When PDIA6 is ablated, insulin release is substantially inhibited (Eletto et al. 2016).

Product Details

Target Species
Human
Western Blotting
Mouse anti PDIA6 detects a band of approximately 50 kDa in HepG2 cell lysates
Product Form
Purified IgG - Liquid
Preparation
Mouse monoclonal antibody affinity purified on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide
Immunogen
E. coli-derived recombinant protein of amino acids 1-440 of human PDIA6
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunised BALB/c mice were fused with cells of the mouse SP2/0 myeloma cell line

Storage Information

Storage
Store undiluted at -20°C, avoiding repeated freeze thaw cycles
Guarantee
12 months from date of despatch

More Information

UniProt
Q15084
Entrez Gene
PDIA6
GO Terms
GO:0005886 plasma membrane
GO:0005515 protein binding
GO:0006457 protein folding
GO:0003756 protein disulfide isomerase activity
GO:0005788 endoplasmic reticulum lumen
GO:0005793 ER-Golgi intermediate compartment
GO:0045454 cell redox homeostasis
GO:0042470 melanosome
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories
Regulatory
For research purposes only

Applications of PDIA6 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/500 1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Mouse IgG (H/L):HRP STAR207P WB 2 ml

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