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PCNA antibody | PC10

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Anti PCNA Antibody, clone PC10 (PrecisionAb Monoclonal Antibody) thumbnail image 1 Anti PCNA Antibody, clone PC10 (PrecisionAb Monoclonal Antibody) thumbnail image 2 Anti PCNA Antibody, clone PC10 (PrecisionAb Monoclonal Antibody) thumbnail image 3
Anti PCNA Antibody, clone PC10 (PrecisionAb Monoclonal Antibody) gallery image 1
Western blot analysis of COS-7 whole cell lysates probed with PCNA antibody (VMA00018) followed by detection with HRP conjugated Goat anti Mouse IgG (1/50,000, STAR207P) and visualized on the ChemiDoc MP with 300 second exposure. Arrow points to PCNA (molecular weight 29 kDa).
Anti PCNA Antibody, clone PC10 (PrecisionAb Monoclonal Antibody) gallery image 2
Western blot analysis of whole cell lysates probed with PCNA antibody (VMA00018) followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000, STAR207P) and visualized on the ChemiDoc MP with 5 second exposure. Arrow points to PCNA (molecular weight 29 kDa).
Anti PCNA Antibody, clone PC10 (PrecisionAb Monoclonal Antibody) gallery image 3
Immunoprecipitation of PCNA from HeLa whole cell lysate. The Input lane is 10% input; the PCNA-IP lane is the immunoprecipitation with the PCNA antibody (2.5 µg, VMA00018); the IgG-IP lane is the immunoprecipitation with Mouse monoclonal IgG (2.5 µg, MCA929). Western blot analysis was performed using PCNA antibody (1/1000, VMA00018) followed by detection with HRP conjugated Goat anti Mouse IgG (1/10000, 1705047) and visualized on the ChemiDoc MP. Arrow points to PCNA.

Mouse anti PCNA

Product Type
PrecisionAb Monoclonal
Clone
PC10
Isotype
IgG2a
Format
Purified
Specificity
PCNA

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Product Code Applications Pack Size List Price Your Price Qty
VMA00018
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
IP WB 100 µl loader
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loader

Mouse anti PCNA antibody recognizes the proliferating cell nuclear antigen, also known as PCNA or cyclin. PCNA is a 261 amino acid nuclear protein vital for cellular DNA synthesis at the replication fork (Li et al. 1995) through its interaction with FEN1 (Wu et al. 1996). PCNA is the auxilliary protein for DNA polymerase δ (Bravo et al. 1987).

PCNA is highly conserved between mammalian species and other vertebrates and this antibody has been shown to detect PCNA in a number of species including rat, mouse and African green monkey

Mouse anti PCNA antibody recognizes proliferating cell nuclear antigen as a single band of ~29 kDa in multiple human cell line lysates by western blotting under reducing conditions.		 PrecisionAb Antibodies

Target Species
Human
Western Blotting
Anti PCNA antibody detects a band of approximately 29 kDa in HeLa cell lysates.
Species Cross-Reactivity
Target SpeciesCross Reactivity
African green monkey
Rat
Mouse
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Mouse monoclonal antibody purified by affinity chromatography on Protein G from tissue culture supernatant.
Buffer Solution
Phosphate buffered saline.
Preservative Stabilisers
0.09% Sodium Azide (NaN3).
Immunogen
Rat PCNA made in the Protein A expression vector pR1T2T
Approx. Protein Concentrations
IgG concentration 0.5 mg/ml.
Regulatory
For research purposes only.
Guarantee
12 months from date of despatch.
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories.

Store undiluted at -20°C, avoiding repeated freeze thaw cycles.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunoprecipitation
Western Blotting 1/1000
The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Description Product Code Applications Pack Size List Price Your Price Quantity
Goat anti Mouse IgG (H/L):HRP STAR207P WB 2 ml loader
List Price Your Price
loader
Description Goat anti Mouse IgG (H/L):HRP

Synonyms
Proliferating Cell Nuclear Antigen
UniProt
P12004
Entrez Gene
PCNA
GO Terms
GO:0000083 regulation of transcription involved in G1/S phase of mitotic cell cycle
GO:0000084 S phase of mitotic cell cycle
GO:0000701 purine-specific mismatch base pair DNA N-glycosylase activity
GO:0000722 telomere maintenance via recombination
GO:0005737 cytoplasm
GO:0005654 nucleoplasm
GO:0005663 DNA replication factor C complex
GO:0043626 PCNA complex
GO:0006271 DNA strand elongation involved in DNA replication
GO:0006275 regulation of DNA replication
GO:0006283 transcription-coupled nucleotide-excision repair
GO:0006297 nucleotide-excision repair, DNA gap filling
GO:0006298 mismatch repair
GO:0008283 cell proliferation
GO:0015630 microtubule cytoskeleton
GO:0048015 phosphatidylinositol-mediated signaling
GO:0019985 translesion synthesis
GO:0030337 DNA polymerase processivity factor activity
GO:0032077 positive regulation of deoxyribonuclease activity
GO:0032139 dinucleotide insertion or deletion binding
GO:0032201 telomere maintenance via semi-conservative replication
GO:0032405 MutLalpha complex binding
GO:0043596 nuclear replication fork
GO:0070557 PCNA-p21 complex

VMA00018

151785 171009

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