Paxillin antibody | RM256

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Rabbit anti Paxillin

Product Type
Monoclonal Antibody
Clone
RM256
Isotype
IgG
Product Code Applications Pack Size List Price Quantity
MCA6310
Datasheet
IC WB
SDS
0.1 ml loader

Rabbit anti Human paxillin antibody recognizes paxillin (PXN). Paxillin is a scaffolding protein, and is a major component of focal adhesions (FAs) required for cell attachment, spreading, and migration. Paxillin is involved with recruiting specific kinases, phosphatases, cofactors, oncoproteins, and structural proteins with downstream roles in intracellular signaling pathways, leading to reorganization of the actin cytoskeleton (Lopez-Colome et al. 2017). The protein shows dynamic changes in expression via protein-protein interaction and phosphorylation. Abnormal paxillin expression correlates with tumor occurrence, invasion, and metastasis of some cancer types (Yang et al. 2017). Overexpression of paxillin has been identified in gastric cancer, prostate cancer, hepatocellular carcinoma, and colorectal carcinoma (Zhao et al. 2015).

Product Details

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse Expected from Sequence
Rat Expected from Sequence
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography of Protein A from animal origin-free culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide
1% Bovine Serum Albumin
50% Glycerol
Immunogen
A peptide corresponding to the N-terminus of Human paxillin

Storage Information

Storage
Store at -20°C only.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use
Guarantee
12 months from date of despatch

More Information

UniProt
P49023
Entrez Gene
PXN
GO Terms
GO:0006936 muscle contraction
GO:0005737 cytoplasm
GO:0005875 microtubule associated complex
GO:0005925 focal adhesion
GO:0007160 cell-matrix adhesion
GO:0007172 signal complex assembly
GO:0007173 epidermal growth factor receptor signaling pathway
GO:0008013 beta-catenin binding
GO:0008270 zinc ion binding
GO:0017166 vinculin binding
GO:0030027 lamellipodium
GO:0034329 cell junction assembly
GO:0034614 cellular response to reactive oxygen species
Regulatory
For research purposes only

Applications of Paxillin antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunocytochemistry 1/1000 1/2000
Western Blotting 1/1000 1/2000
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Rabbit IgG (Fc):Biotin STAR121B E WB 1 mg loader
Goat anti Rabbit IgG (Fc):FITC STAR121F F 1 mg loader
Goat anti Rabbit IgG (Fc):HRP STAR121P E WB 1 mg loader
Sheep anti Rabbit IgG:FITC STAR34B C F 1 mg loader
Sheep anti Rabbit IgG:DyLight®488 STAR36D488GA F IF WB 0.1 mg
Sheep anti Rabbit IgG:DyLight®680 STAR36D680GA F IF WB 0.1 mg
Sheep anti Rabbit IgG:Dylight®800 STAR36D800GA F IF WB 0.1 mg
Goat anti Rabbit IgG (H/L):HRP STAR124P C E WB 1 mg loader
Sheep anti Rabbit IgG:RPE STAR35A F 1 ml loader

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