PAK4 antibody | OTI1C7

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Mouse anti PAK4

Product Type
PrecisionAb Monoclonal
Clone
OTI1C7
Isotype
IgG1
Format
Purified
Product Code Applications Pack Size List Price Quantity
VMA00206
Datasheet
WB
MSDS
100 µl loader

Mouse anti Human PAK4 antibody recognizes PAK4, also known as p21(CDKN1A)-activated kinase 4, p21-activated kinase 4, protein kinase related to S. cerevisiae STE20, effector for Cdc42Hs and serine/threonine-protein kinase PAK4.

PAK proteins, a family of serine/threonine p21-activating kinases, include PAK1, PAK2, PAK3 and PAK4. These proteins are critical effectors that link Rho GTPases to cytoskeleton reorganization and nuclear signaling. They serve as targets for the small GTP binding proteins Cdc42 and Rac and have been implicated in a wide range of biological activities. PAK4 interacts specifically with the GTP-bound form of Cdc42Hs and weakly activates the JNK family of MAP kinases. PAK4 is a mediator of filopodia formation and may play a role in the reorganization of the actin cytoskeleton. Multiple alternatively spliced transcript variants encoding distinct isoforms have been found for PAK4 (provided by RefSeq, Jul 2008).

Mouse anti Human PAK4 antibody detects a band of 72 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species
Human
Western Blotting
Anti PAK4 detects a band of approximately 72 kDa in K562 cell lysates.
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Mouse monoclonal antibody purified by affinity chromatography from ascites.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
50% Glycerol
Immunogen
Recombinant protein corresponding to amino acids 1-308 of human PAK4 (NP_005875) produced in E. coli

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Guarantee
As supplied, 12 months from date of despatch.

More Information

UniProt
O96013 Related reagents
Entrez Gene
PAK4 Related reagents
GO Terms
GO:0005515 protein binding
GO:0004674 protein serine/threonine kinase activity
GO:0007165 signal transduction
GO:0005524 ATP binding
GO:0005794 Golgi apparatus
GO:0006468 protein phosphorylation
GO:0006928 cellular component movement
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories.
Regulatory
For research purposes only

Applications of PAK4 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Mouse IgG (H/L):HRP STAR207P WB 2 ml

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