PAK1 antibody | CD04/3E5

Mouse anti PAK1

Product Type
PrecisionAb Monoclonal
Clone
CD04/3E5
Isotype
IgG1
Format
Purified
Specificity
PAK1

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VMA00700
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Mouse anti Human PAK1 antibody recognizes the serine/threonine-protein kinase PAK1, also known as alpha-PAK, p21/Cdc42/Rac1-activated kinase 1 and p65-PAK.

PAK1 encodes a family member of serine/threonine p21-activating kinases, known as PAK proteins. These proteins are critical effectors that link RhoGTPases to cytoskeleton reorganization and nuclear signaling, and they serve as targets for the small GTP binding proteins Cdc42 and Rac. This specific family member regulates cell motility and morphology. Alternatively spliced transcript variants encoding different isoforms have been found for PAK1 (provided by RefSeq, Apr 2010).

Mouse anti Human PAK1 antibody detects a band of 60 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
PrecisionAb Antibodies

Target Species
Human
Western Blotting
Mouse anti PAK1 detects a band of approximately 60 kDa in Jurkat cell lysates
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - Liquid
Preparation
Mouse monoclonal antibody affinity purified on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide
Immunogen
E. coli-derived recombinant protein of amino acids 245-545 of human PAK1
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunised BALB/c mice were fused with cells of the mouse SP2/0 myeloma cell line
Regulatory
For research purposes only
Guarantee
12 months from date of despatch
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories.

Store undiluted at -20°C, avoiding repeated freeze thaw cycles

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry
Western Blotting 1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

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Goat anti Mouse IgG (H/L):HRP STAR207P WB 2 ml loader
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Description Goat anti Mouse IgG (H/L):HRP

UniProt
Q13153
Entrez Gene
PAK1
GO Terms
GO:0005515 protein binding
GO:0007010 cytoskeleton organization
GO:0006915 apoptosis
GO:0005829 cytosol
GO:0004674 protein serine/threonine kinase activity
GO:0005518 collagen binding
GO:0005524 ATP binding
GO:0005794 Golgi apparatus
GO:0005925 focal adhesion
GO:0006984 ER-nucleus signaling pathway
GO:0007411 axon guidance
GO:0031295 T cell costimulation
GO:0043507 positive regulation of JUN kinase activity
GO:0046777 protein autophosphorylation
GO:0050852 T cell receptor signaling pathway
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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