PAK1 antibody | CD04/3E5

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Mouse anti PAK1

PAK1 antibody recognizes the serine/threonine-protein kinase PAK 1, also known as alpha-PAK, p21/Cdc42/Rac1-activated kinase 1 , or p65-PAK.

PAK1 Antibody with Control Lysate

PAK1 antibody recognizes the serine/threonine-protein kinase PAK 1, also known as alpha-PAK, p21/Cdc42/Rac1-activated kinase 1 , or p65-PAK.

Product Type
PrecisionAb™ Monoclonal
Clone
CD04/3E5
Isotype
IgG1
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
VMA00700 WB 100 µl
VMA00700KT WB 2 Westerns
With Lysate 400µg
Mouse anti Human PAK1 antibody recognizes the serine/threonine-protein kinase PAK1, also known as alpha-PAK, p21/Cdc42/Rac1-activated kinase 1 and p65-PAK.

PAK1 encodes a family member of serine/threonine p21-activating kinases, known as PAK proteins. These proteins are critical effectors that link RhoGTPases to cytoskeleton reorganization and nuclear signaling, and they serve as targets for the small GTP binding proteins Cdc42 and Rac. This specific family member regulates cell motility and morphology. Alternatively spliced transcript variants encoding different isoforms have been found for PAK1 (provided by RefSeq, Apr 2010).

Mouse anti Human PAK1 antibody detects a band of 60 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - Liquid
Product Form
Purified IgG - Liquid
Preparation
Mouse monoclonal antibody affinity purified on Protein G from tissue culture supernatant
Preparation
20μl Mouse monoclonal antibody affinity purified on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide
Preservative Stabilisers
0.09% Sodium Azide
Immunogen
E. coli-derived recombinant protein of amino acids 245-545 of human PAK1
Fusion Partners
Spleen cells from immunised BALB/c mice were fused with cells of the mouse SP2/0 myeloma cell line

Storage Information

Storage
Store undiluted at -20°C, avoiding repeated freeze thaw cycles
Storage
Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
Shelf Life
As supplied, 12 months from date of despatch
Shelf Life
As supplied, 12 months from date of despatch

More Information

UniProt
Q13153 Related reagents
Entrez Gene
PAK1 Related reagents
GO Terms
GO:0005515 protein binding
GO:0007010 cytoskeleton organization
GO:0006915 apoptosis
GO:0005829 cytosol
GO:0004674 protein serine/threonine kinase activity
GO:0005518 collagen binding
GO:0005524 ATP binding
GO:0005794 Golgi apparatus
GO:0005925 focal adhesion
GO:0006984 ER-nucleus signaling pathway
GO:0007411 axon guidance
GO:0031295 T cell costimulation
GO:0043507 positive regulation of JUN kinase activity
GO:0046777 protein autophosphorylation
GO:0050852 T cell receptor signaling pathway
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories
Regulatory
For research purposes only

Applications of PAK1 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000
Western Blotting 1/1000

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
Western Blotting
Mouse anti PAK1 detects a band of approximately 60 kDa in Jurkat cell lysates
Western Blotting
Mouse anti PAK1 detects a band of approximately 60 kDa in Jurkat cell lysates
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Goat anti Mouse IgG (H/L):HRP STAR207P 2 ml WB
Goat anti Mouse IgG (H/L):HRP STAR207P 2 ml WB