PAG antibody

100% Secure


Mouse anti PAG

PAG antibody recognizes phosphoprotein associated with glycosphingolipid-enriched microdomains 1, also known as Csk-binding protein, transmembrane adapter protein, PAG, or transmembrane phosphoprotein Cbp. The antibody has been extensively validated for western blotting using whole cell lysates.

PAG Antibody with Control Lysate

PAG antibody recognizes phosphoprotein associated with glycosphingolipid-enriched microdomains 1, also known as Csk-binding protein, transmembrane adapter protein, PAG, or transmembrane phosphoprotein Cbp. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Type
PrecisionAb™ Monoclonal
Isotype
IgG2a
Format
Purified

Select your package type:

Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
VMA00028 WB 100 µl
VMA00028KT WB 2 Westerns
With Lysate 400µg
Mouse anti Human PAG antibody recognizes human phosphoprotein associated with glycosphingolipid-enriched microdomains (PAG), also known as Csk-binding protein (Cbp) or transmembrane phosphoprotein Cbp. PAG is a single pass type III transmembrane protein. Mouse anti Human PAG antibody recognizes an epitope located in the cytoplasmic domain which localizes to membrane lipid rafts and is involved in regulation of T-Cell activation.

The engagement of immunoreceptors with natural ligands or antibodies is the initiating factor in a long line of events down a cytosolic signaling cascade. The transmission of signals along the various pathways within this cascade requires the phosphorylation of various substrates, by members of the src and syk families of protein tyrosine kinases (PTKs).

Evidence has shown that by binding to the SH2 domain of the protein tyrosine kinase Csk, a major negative regulator of src-family kinases, PAG acts not only acts as a ‘co-factor’ for relocating Csk to substrate rich microdomains, but also increases the affinity of Csk for src-family kinases, through the formation of PAG-Csk complexes.

Mouse anti Human PAG antibody recognizes human PAG as a single band of ~80 kDa in human cell line lysates by Western blotting under reducing conditions.

Product Details

Target Species
Human
Western Blotting
Anti PAG antibody detects a band of approximately 80 kDa in Raji cell lysates.
Product Form
Purified IgG - liquid
Product Form
Purified IgG - liquid
Preparation
Mouse monoclonal antibody purified by affinity chromatography on Protein A from ascites
Preparation
20μl Mouse monoclonal antibody purified by affinity chromatography on Protein A from ascites
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
Recombinant protein corresponding to amino acids 97 - 432 of human Cbp/PAG
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.

Positive Control Lysate

Lysate Composition
400μg Raji lysate lyophilized in RIPA buffer.
Lysate Reconstitution
- If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
- If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Storage
Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
Shelf Life
As supplied, 12 months from date of despatch.
Shelf Life
As supplied, 12 months from date of despatch.

More Information

UniProt
Q9NWQ8 Related reagents
Entrez Gene
PAG1 Related reagents
GO Terms
GO:0005886 plasma membrane
GO:0016021 integral to membrane
GO:0035556 intracellular signal transduction
GO:0005070 SH3/SH2 adaptor activity
GO:0007173 epidermal growth factor receptor signaling pathway
GO:0042169 SH2 domain binding
GO:0045121 membrane raft
GO:0050852 T cell receptor signaling pathway
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Regulatory
For research purposes only

Applications of PAG antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000
Western Blotting 1/1000

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Goat anti Mouse IgG (H/L):HRP STAR207P 2 ml WB
Goat anti Mouse IgG (H/L):HRP STAR207P 2 ml WB

Western Blotting without Trial and Error: Use PrecisionAb™ Antibodies

Watch how PrecisionAb Validated Western Blotting Antibodies are defining a new standard of quality that you can trust to deliver superior results and reproducibility in western blotting.

Contact Information


Online Payment Methods:

  1. Invoice / Purchase Order
  2. Credit Card (Maestro, VISA,MasterCard & VISA Electron)
credit cards accepted