PAG antibody

Mouse anti PAG

Product Type
PrecisionAb Monoclonal
Isotype
IgG2a
Format
Purified
Specificity
PAG

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VMA00028
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Mouse anti Human PAG antibody recognizes human phosphoprotein associated with glycosphingolipid-enriched microdomains (PAG), also known as Csk-binding protein (Cbp) or transmembrane phosphoprotein Cbp. PAG is a single pass type III transmembrane protein. Mouse anti Human PAG antibody recognizes an epitope located in the cytoplasmic domain which localizes to membrane lipid rafts and is involved in regulation of T-Cell activation.

The engagement of immunoreceptors with natural ligands or antibodies is the initiating factor in a long line of events down a cytosolic signaling cascade. The transmission of signals along the various pathways within this cascade requires the phosphorylation of various substrates, by members of the src and syk families of protein tyrosine kinases (PTKs).

Evidence has shown that by binding to the SH2 domain of the protein tyrosine kinase Csk, a major negative regulator of src-family kinases, PAG acts not only acts as a ‘co-factor’ for relocating Csk to substrate rich microdomains, but also increases the affinity of Csk for src-family kinases, through the formation of PAG-Csk complexes.

Mouse anti Human PAG antibody recognizes human PAG as a single band of ~80 kDa in human cell line lysates by Western blotting under reducing conditions.
PrecisionAb Antibodies

Target Species
Human
Western Blotting
Anti PAG antibody detects a band of approximately 80 kDa in Raji cell lysates.
Product Form
Purified IgG - liquid
Preparation
Mouse monoclonal antibody purified by affinity chromatography on Protein A from ascites.
Buffer Solution
Phosphate buffered saline.
Preservative Stabilisers
0.09% Sodium Azide (NaN3).
Immunogen
Recombinant protein corresponding to amino acids 97 - 432 of human Cbp/PAG
Regulatory
For research purposes only.
Guarantee
12 months from date of despatch.
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories.

Store undiluted at -20°C, avoiding repeated freeze thaw cycles.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000
The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

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Goat anti Mouse IgG (H/L):HRP STAR207P WB 2 ml loader
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Description Goat anti Mouse IgG (H/L):HRP

Synonyms
CSK Binding Protein
UniProt
Q9NWQ8
Entrez Gene
PAG1
GO Terms
GO:0005886 plasma membrane
GO:0016021 integral to membrane
GO:0035556 intracellular signal transduction
GO:0005070 SH3/SH2 adaptor activity
GO:0007173 epidermal growth factor receptor signaling pathway
GO:0042169 SH2 domain binding
GO:0045121 membrane raft
GO:0050852 T cell receptor signaling pathway
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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