P90RSK1 (pThr359/pSer363) antibody | RM233

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Rabbit anti P90RSK1 (pThr359/pSer363)

Product Type
Monoclonal Antibody
Clone
RM233
Isotype
IgG
Product Code Applications Pack Size List Price Quantity
MCA6299
Datasheet
P WB
SDS
0.1 ml loader

Rabbit anti Human P90RSK1 (pThr359/pSer363) antibody recognizes ribosomal protein S6 kinase alpha-1 (S6K-alpha-1), also known as RPS6KA1, RSK1, MAPKAPK1A when phosphorylated at threonine 359 and serine 363. P90RSK1 belongs to the RSK (ribosomal S6 kinase) family of serine/threonine kinases (Fisher and Blenis 1996). P90RSK1 is a downstream effector of the Ras/ERK signalling pathway (Casalvieri et al. 2017). It directly phosphorylates IkappaBbeta and activates NF-kappaB (Xu et al. 2006). P90RSK1 has been reported to phosphorylate CREB on Ser133 (Cho et al. 2011). RSK also promotes mTOR signaling and translation (Shahbazian et al. 2006). Expression and activity of RSK1 promotes tumor growth and survival (Casalvieri et al. 2017). Phosphorylation of P90RSK1, including at Thr359 and Ser363, activates P90RSK1 in the cytoplasm and at the plasma membrane, and fully active RSK1 localizes to the nucleus (Gao et al. 2012).

Product Details

Target Species
Human
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography of Protein A from animal origin-free culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide
1% Bovine Serum Albumin
50% Glycerol
Immunogen
A phospho-peptide corresponding to Human P90RSK1 (pThr359/Ser363)

Storage Information

Storage
Store at -20°C only.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use
Guarantee
12 months from date of despatch

More Information

UniProt
Q15418
Entrez Gene
RPS6KA1
GO Terms
GO:0005515 protein binding
GO:0000287 magnesium ion binding
GO:0002755 MyD88-dependent toll-like receptor signaling pathway
GO:0002756 MyD88-independent toll-like receptor signaling pathway
GO:0005829 cytosol
GO:0004674 protein serine/threonine kinase activity
GO:0005524 ATP binding
GO:0005654 nucleoplasm
GO:0006468 protein phosphorylation
GO:0007268 synaptic transmission
GO:0007411 axon guidance
GO:0008063 Toll signaling pathway
GO:0034130 toll-like receptor 1 signaling pathway
GO:0034134 toll-like receptor 2 signaling pathway
GO:0034138 toll-like receptor 3 signaling pathway
GO:0034142 toll-like receptor 4 signaling pathway
GO:0045087 innate immune response
GO:0048011 nerve growth factor receptor signaling pathway
GO:0051403 stress-activated MAPK cascade
Regulatory
For research purposes only

Applications of P90RSK1 (pThr359/pSer363) antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunohistology - Paraffin 1/200 1/500
Western Blotting 1/1000 1/2000
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Rabbit IgG (Fc):Biotin STAR121B E WB 1 mg loader
Goat anti Rabbit IgG (Fc):FITC STAR121F F 1 mg loader
Goat anti Rabbit IgG (Fc):HRP STAR121P E WB 1 mg loader
Goat anti Rabbit IgG (H/L):HRP STAR124P C E WB 1 mg loader
Sheep anti Rabbit IgG:FITC STAR34B C F 1 mg loader
Sheep anti Rabbit IgG:RPE STAR35A F 1 ml loader
Sheep anti Rabbit IgG:DyLight®488 STAR36D488GA F IF WB 0.1 mg
Sheep anti Rabbit IgG:DyLight®680 STAR36D680GA F IF WB 0.1 mg
Sheep anti Rabbit IgG:Dylight®800 STAR36D800GA F IF WB 0.1 mg

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