p38 MAPK antibody | RM245

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Rabbit anti p38 MAPK

Product Type
Monoclonal Antibody
Clone
RM245
Isotype
IgG
Product Code Applications Pack Size List Price Quantity
MCA6304
Datasheet
P WB
SDS
0.1 ml loader

Rabbit anti Human MAPK14 antibody recognizes mitogen-activated protein kinase 14, also known as p38alpha, CSBP, MXI2, SAPK2A. MAPK14 is a member of th p38 MAP kinase (MAPK) family, and is a stress-activated serine/threonine protein kinase involved with inflammation. MAPK14 is activated in response to extracellular stress and helps regulate transcription and translation of specific genes, allowing a cell to fine-tune its response. Within the immune system, MAPK14 promotes production of pro-inflammatory cytokines from macrophages, and helps determine the strength and duration of the inflammatory response (Alam and Scheper 2016). MAPK14 is able to regulate a diverse range of biological processes, including cell proliferation, differentiation, apoptosis, senescence, and inflammation (Wu et al. 2019). In colon cancer, MAPK14 has been shown to be required for cell proliferation and survival, and its inhibition leads to cell cycle arrest and autophagy-mediated cell death (Paillas et al. 2012). MAPK14 inhibition has been suggested as a treatment for several inflammatory diseases, including Chron's disease and rheumatoid arthritis (She et al. 2018).

Product Details

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Rat Expected from Sequence
Mouse Expected from Sequence
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography of Protein A from animal origin-free culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide
1% Bovine Serum Albumin
50% Glycerol
Immunogen
A peptide corresponding to Human p38 MAPK

Storage Information

Storage
Store at -20°C only.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use
Guarantee
12 months from date of despatch

More Information

UniProt
Q16539
P53778
Entrez Gene
MAPK12
MAPK14
GO Terms
GO:0005515 protein binding
GO:0000287 magnesium ion binding
GO:0005739 mitochondrion
GO:0004707 MAP kinase activity
GO:0005524 ATP binding
GO:0005654 nucleoplasm
GO:0006975 DNA damage induced protein phosphorylation
GO:0007050 cell cycle arrest
GO:0007265 Ras protein signal transduction
GO:0007517 muscle organ development
GO:0045445 myoblast differentiation
GO:0048011 nerve growth factor receptor signaling pathway
GO:0051149 positive regulation of muscle cell differentiation
GO:0000187 activation of MAPK activity
GO:0002755 MyD88-dependent toll-like receptor signaling pathway
GO:0002756 MyD88-independent toll-like receptor signaling pathway
GO:0005829 cytosol
GO:0004708 MAP kinase kinase activity
GO:0006928 cellular component movement
GO:0006935 chemotaxis
GO:0008063 Toll signaling pathway
GO:0010670 positive regulation of oxygen and reactive oxygen species metabolic process
GO:0016071 mRNA metabolic process
GO:0030168 platelet activation
GO:0034130 toll-like receptor 1 signaling pathway
GO:0034134 toll-like receptor 2 signaling pathway
GO:0034138 toll-like receptor 3 signaling pathway
GO:0034142 toll-like receptor 4 signaling pathway
GO:0042692 muscle cell differentiation
GO:0042770 signal transduction in response to DNA damage
GO:0045087 innate immune response
GO:0051090 regulation of transcription factor activity
GO:0051403 stress-activated MAPK cascade
GO:0071479 cellular response to ionizing radiation
GO:0090400 stress-induced premature senescence
Regulatory
For research purposes only

Applications of p38 MAPK antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunohistology - Paraffin 1/1000 1/5000
Western Blotting 1/1000 1/2000
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Rabbit IgG (Fc):Biotin STAR121B E WB 1 mg loader
Goat anti Rabbit IgG (Fc):FITC STAR121F F 1 mg loader
Goat anti Rabbit IgG (Fc):HRP STAR121P E WB 1 mg loader
Goat anti Rabbit IgG (H/L):HRP STAR124P C E WB 1 mg loader
Sheep anti Rabbit IgG:FITC STAR34B C F 1 mg loader
Sheep anti Rabbit IgG:RPE STAR35A F 1 ml loader
Sheep anti Rabbit IgG:DyLight®488 STAR36D488GA F IF WB 0.1 mg
Sheep anti Rabbit IgG:DyLight®680 STAR36D680GA F IF WB 0.1 mg
Sheep anti Rabbit IgG:Dylight®800 STAR36D800GA F IF WB 0.1 mg

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