P23 antibody | OTI2D2

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Mouse anti P23

Mouse anti human P23 antibody recognizes prostaglandin E synthase 3, also known as PTGES3, Hsp90 co-chaperone, cPGES, cytosolic prostaglandin E2 synthase, or telomerase-binding protein p23. This antibody has been extensively validated for western blotting using whole cell lysates.

P23 Antibody with Control Lysate

Mouse anti human P23 antibody recognizes prostaglandin E synthase 3, also known as PTGES3, Hsp90 co-chaperone, cPGES, cytosolic prostaglandin E2 synthase, progesterone receptor complex p23, prostaglandin E synthase 3 or telomerase-binding protein p23.

Product Type
PrecisionAb™ Monoclonal
Clone
OTI2D2
Isotype
IgG1
Format
Purified

Select your package type:

Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
VMA00432 WB 100 µl
VMA00432KT WB 2 Westerns
With Lysate 400µg
Mouse anti Human P23 antibody recognizes the P23, also known as PTGES3, Hsp90 co-chaperone, cytosolic prostaglandin E synthase, progesterone receptor complex p23, telomerase-binding protein p23 and unactive progesterone receptor, 23 kD.

Mouse anti Human P23 antibody detects a band of 23 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species
Human
Western Blotting
Anti P23 detects a band of approximately 23 kDa in Jurkat cell lysates
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Product Form
Purified IgG - liquid
Preparation
Mouse monoclonal antibody purified by affinity chromatography from ascites
Preparation
20μl Mouse monoclonal antibody purified by affinity chromatography from ascites
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
50% Glycerol
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
50% Glycerol
Immunogen
Full length recombinant human P23 (NP_006592) produced in E.coli
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.

Positive Control Lysate

Lysate Composition
400μg Jurkat lysate lyophilized in RIPA buffer
Lysate Reconstitution
- If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
- If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Storage
Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
Shelf Life
As supplied, 12 months from date of despatch.
Shelf Life
As supplied, 12 months from date of despatch

More Information

UniProt
Q15185 Related reagents
Entrez Gene
PTGES3 Related reagents
GO Terms
GO:0051082 unfolded protein binding
GO:0000723 telomere maintenance
GO:0000781 chromosome, telomeric region
GO:0001516 prostaglandin biosynthetic process
GO:0003720 telomerase activity
GO:0005829 cytosol
GO:0007165 signal transduction
GO:0005697 telomerase holoenzyme complex
GO:0042446 hormone biosynthetic process
GO:0050220 prostaglandin-E synthase activity
GO:0070389 chaperone cofactor-dependent protein refolding
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories
Regulatory
For research purposes only

Applications of P23 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000
Western Blotting 1/1000

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Goat anti Mouse IgG (H/L):HRP STAR207P 2 ml WB
Goat anti Mouse IgG (H/L):HRP STAR207P 2 ml WB

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