Nuclear Cap Binding Protein Subunit 1 antibody | EF03/2E5-3

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Mouse anti Nuclear Cap Binding Protein Subunit 1

Product Type
PrecisionAb Monoclonal
Clone
EF03/2E5-3
Isotype
IgG2b
Format
Purified
Product Code Applications Pack Size List Price Quantity
VMA00885
Datasheet
WB
SDS
100 µl loader

Mouse anti nuclear cap binding protein subunit 1 antibody recognizes nuclear cap-binding protein subunit 1, also known as CBP80.

The adaptor protein NCPB1, along with NCBP2, comprise the cap-binding complex (CBC). The CBC binds the RNA cap and orchestrates multiple stages of RNA processing including pre-mRNA splicing, 3'-end processing, and recruitment of translation factors in the cytoplasm (Gebhardt et al. 2015). Nuclear cap binding protein subunit 1 promotes growth of lung cancer cells, wound healing ability, migration and epithelial-mesenchymal transition, and nuclear cap binding protein subunit 1 is overexpressed in several lung cancer tissues and cell lines. Silencing of nuclear cap binding protein subunit 1 in HeLa cells has been found to reduce cell growth rate (Zhang et al. 2019).

Product Details

Target Species
Human
Western Blotting
Mouse anti nuclear cap binding protein subunit 1 antibody detects a band of approximately 81 kDa in HEK293 cell lysates
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - Liquid
Preparation
Mouse monoclonal antibody affinity purified on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide
Immunogen
E. coli-derived recombinant protein of amino acids 1-528 of human nuclear cap binding protein subunit 1
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunised BALB/c mice were fused with cells of the mouse SP2/0 myeloma cell line

Storage Information

Storage
Store undiluted at -20°C, avoiding repeated freeze thaw cycles
Guarantee
12 months from date of despatch

More Information

UniProt
Q09161
Entrez Gene
NCBP1
GO Terms
GO:0000184 nuclear-transcribed mRNA catabolic process, nonsense-mediated decay
GO:0005515 protein binding
GO:0000339 RNA cap binding
GO:0000387 spliceosomal snRNP assembly
GO:0030529 ribonucleoprotein complex
GO:0005829 cytosol
GO:0005654 nucleoplasm
GO:0005845 mRNA cap binding complex
GO:0006368 transcription elongation from RNA polymerase II promoter
GO:0006369 termination of RNA polymerase II transcription
GO:0006370 mRNA capping
GO:0006379 mRNA cleavage
GO:0006406 mRNA export from nucleus
GO:0006446 regulation of translational initiation
GO:0008334 histone mRNA metabolic process
GO:0016032 viral reproduction
GO:0031047 gene silencing by RNA
GO:0031124 mRNA 3'-end processing
GO:0031442 positive regulation of mRNA 3'-end processing
GO:0034660 ncRNA metabolic process
GO:0050434 positive regulation of viral transcription
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories
Regulatory
For research purposes only

Applications of Nuclear Cap Binding Protein Subunit 1 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Mouse IgG (H/L):HRP STAR207P WB 2 ml

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