Rabbit anti Human NFKB p105/p50 antibody recognizes nuclear factor NF-kappa-B p105 subunit, also known as NFKB1, DNA-binding factor KBF1, EBP-1. NFKB is a transcription factor divided into a family of subunits. NFKB1 is synthesized as a longer precursor protein (p105) that can be proteolytically cleaved into a shorter, DNA-binding form (p50) which can bind other NFKB subunits and elicit a variety of transcriptional responses. p65:p50 heterodimers activate the NFKB response and drive inflammation, whereas p50:p50 homodimers are critical suppressors of the NFKB response (Cartwright et al. 2016). Dysregulated NFKB signaling is strongly associated with carcinogenesis, and NFKB1 can either act as a tumor promoter or suppressor depending on its interactions. p65:p50 hoterodimers are generally pro-inflammatory and pro-tumorigenic, whereas p50:p50 homodimers can have a tumor suppressive effect (Concetti and Wilson 2018).
- Target Species
- Species Cross-Reactivity
|Target Species||Cross Reactivity|
||Expected from Sequence
||Expected from Sequence
- N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Purified IgG prepared by affinity chromatography of Protein A from animal origin-free culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide
1% Bovine Serum Albumin
- A peptide corresponding to residues in Human NF?B p105/p50
- Store at -20°C only.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use
- 12 months from date of despatch
- Entrez Gene
- GO Terms
MyD88-dependent toll-like receptor signaling pathway
MyD88-independent toll-like receptor signaling pathway
sequence-specific DNA binding transcription factor activity
transcription from RNA polymerase II promoter
Toll signaling pathway
negative regulation of gene-specific transcription from RNA polymerase II promoter
positive regulation of macrophage derived foam cell differentiation
positive regulation of lipid storage
negative regulation of calcidiol 1-monooxygenase activity
negative regulation of vitamin D biosynthetic process
transcription activator activity
membrane protein intracellular domain proteolysis
negative regulation of cellular protein metabolic process
negative regulation of cholesterol transport
toll-like receptor 1 signaling pathway
toll-like receptor 2 signaling pathway
toll-like receptor 3 signaling pathway
toll-like receptor 4 signaling pathway
innate immune response
nerve growth factor receptor signaling pathway
T cell receptor signaling pathway
positive regulation of NF-kappaB transcription factor activity
positive regulation of canonical Wnt receptor signaling pathway
- For research purposes only
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Applications of NFkB p105/p50 antibody
|Immunohistology - Paraffin
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
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Secondary Antibodies Available
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