Neuron Specific Enolase antibody
Mouse anti Human neuron specific enolase antibody recognizes neuron specific enolase (NSE), also known as 2-phospho-D-glycerate hydro-lyase, 2-phospho-D-glycerate hydrolyase, epididymis secretory protein Li 279, gamma-enolase and neuron specific gamma enolase.
Encoded by the ENO2 gene, neuron specific enolase is one of the three enolase isoenzymes found in mammals. This isoenzyme, a homodimer, is found in mature neurons and cells of neuronal origin. A switch from alpha enolase to gamma enolase occurs in neural tissue during development in rats and primates (provided by RefSeq, Jul 2008).
Mouse anti Human neuron specific enolase antibody detects a band of 47 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
Encoded by the ENO2 gene, neuron specific enolase is one of the three enolase isoenzymes found in mammals. This isoenzyme, a homodimer, is found in mature neurons and cells of neuronal origin. A switch from alpha enolase to gamma enolase occurs in neural tissue during development in rats and primates (provided by RefSeq, Jul 2008).
Mouse anti Human neuron specific enolase antibody detects a band of 47 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
Product Details
- Target Species
- Human
- Western Blotting
- Anti Neuron Specific Enolase detects a band of approximately 47 kDa in Jurkat cell lysates.
- Product Form
- Purified IgG - liquid
- Preparation
- Mouse monoclonal antibody purified by affinity chromatography on Protein G from ascites
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Immunogen
- Purified recombinant fragment, amino acids 251-433, of human neuron specific enolase expressed in E. coli
Storage Information
- Storage
- Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
- Guarantee
- 12 months from date of despatch
More Information
- UniProt
- P09104
- Entrez Gene
- ENO2
- GO Terms
- GO:0000015 phosphopyruvate hydratase complex
- GO:0005886 plasma membrane
- GO:0000287 magnesium ion binding
- GO:0004634 phosphopyruvate hydratase activity
- GO:0006094 gluconeogenesis
- GO:0006096 glycolysis
- Acknowledgements
- PrecisionAb is a trademark of Bio-Rad Laboratories.
- Regulatory
- For research purposes only
Applications of Neuron Specific Enolase antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Western Blotting | 1/1000 |
The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.
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