Neuron Specific Enolase antibody

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Mouse anti Neuron Specific Enolase

Neuron specific enolase antibody recognizes the gamma-enolase protein, also known as 2-phospho-D-glycerate hydrolyase, epididymis secretory protein Li 279 or neuron specific gamma enolase. This antibody has been extensively validated for western blotting using whole cell lysates.

Neuron Specific Enolase Antibody with Control Lysate

Neuron specific enolase antibody recognizes the gamma-enolase protein, also known as 2-phospho-D-glycerate hydrolyase, epididymis secretory protein Li 279 or neuron specific gamma enolase. This antibody has been extensively validated for western blotting using whole cell lysates.

Product Type
PrecisionAb™ Monoclonal
Isotype
IgG1
Format
Purified

Select your package type:

Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
VMA00152 WB 100 µl
VMA00152KT WB 2 Westerns
With Lysate 400µg
Mouse anti Human neuron specific enolase antibody recognizes neuron specific enolase (NSE), also known as 2-phospho-D-glycerate hydro-lyase, 2-phospho-D-glycerate hydrolyase, epididymis secretory protein Li 279, gamma-enolase and neuron specific gamma enolase.

Encoded by the ENO2 gene, neuron specific enolase is one of the three enolase isoenzymes found in mammals. This isoenzyme, a homodimer, is found in mature neurons and cells of neuronal origin. A switch from alpha enolase to gamma enolase occurs in neural tissue during development in rats and primates (provided by RefSeq, Jul 2008).

Mouse anti Human neuron specific enolase antibody detects a band of 47 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species
Human
Western Blotting
Anti Neuron Specific Enolase detects a band of approximately 47 kDa in Jurkat cell lysates.
Product Form
Purified IgG - liquid
Product Form
Purified IgG - liquid
Preparation
Mouse monoclonal antibody purified by affinity chromatography on Protein G from ascites
Preparation
20μl Mouse monoclonal antibody prepared by affinity chromatography on Protein G from ascites
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
Purified recombinant fragment, amino acids 251-433, of human neuron specific enolase expressed in E. coli
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.

Positive Control Lysate

Lysate Composition
400μg Jurkat lysate lyophilized in RIPA buffer.
Lysate Reconstitution
- If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
- If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Storage
Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
Shelf Life
As supplied, 12 months from date of despatch.
Shelf Life
As supplied, 12 months from date of despatch.

More Information

UniProt
P09104 Related reagents
Entrez Gene
ENO2 Related reagents
GO Terms
GO:0000015 phosphopyruvate hydratase complex
GO:0005886 plasma membrane
GO:0000287 magnesium ion binding
GO:0004634 phosphopyruvate hydratase activity
GO:0006094 gluconeogenesis
GO:0006096 glycolysis
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Regulatory
For research purposes only

Applications of Neuron Specific Enolase antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000
Western Blotting 1/1000

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Goat anti Mouse IgG (H/L):HRP STAR207P 2 ml WB
Goat anti Mouse IgG (H/L):HRP STAR207P 2 ml WB

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