Neuron Specific Enolase antibody

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Anti Neuron Specific Enolase Antibody (PrecisionAb Monoclonal Antibody) gallery image 1 — Western blot analysis of whole cell lysates probed with Mouse anti Human neuron specific enolase antibody (**VMA00152**) followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000, STAR207P) and visualized on the ChemiDoc MP with 17 second exposure. Arrow points to neuron specific enolase (molecular weight 47 kDa).

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Mouse anti Neuron Specific Enolase

Product Type: PrecisionAb Monoclonal
Isotype: IgG1
Format: Purified
Specificity: Neuron Specific Enolase

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Product Code Applications Pack Size List Price Your Price Qty

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VMA00152 Datasheet Datasheet SDS SDS	WB	100 µl		Log in	Get Quote
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Mouse anti Human neuron specific enolase antibody recognizes neuron specific enolase (NSE), also known as 2-phospho-D-glycerate hydro-lyase, 2-phospho-D-glycerate hydrolyase, epididymis secretory protein Li 279, gamma-enolase and neuron specific gamma enolase.

Encoded by the ENO2 gene, neuron specific enolase is one of the three enolase isoenzymes found in mammals. This isoenzyme, a homodimer, is found in mature neurons and cells of neuronal origin. A switch from alpha enolase to gamma enolase occurs in neural tissue during development in rats and primates (provided by RefSeq, Jul 2008).

Mouse anti Human neuron specific enolase antibody detects a band of 47 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Target Species: Human
Western Blotting: Anti Neuron Specific Enolase detects a band of approximately 47 kDa in Jurkat cell lysates.
Product Form: Purified IgG - liquid
Preparation: Mouse monoclonal antibody purified by affinity chromatography on Protein G from ascites.
Buffer Solution: Phosphate buffered saline.
Preservative Stabilisers: 0.09% Sodium Azide (NaN₃).
Immunogen: Purified recombinant fragment, amino acids 251-433, of human neuron specific enolase expressed in E. coli
Regulatory: For research purposes only.
Guarantee: 12 months from date of despatch.
Acknowledgements: PrecisionAb is a trademark of Bio-Rad Laboratories.

Store undiluted at -20°C, avoiding repeated freeze thaw cycles.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Western Blotting		1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Description	Product Code	Applications	Pack Size	Your Price
Goat anti Mouse IgG (H/L):HRP	STAR207P	WB	2 ml	Log in	Get Quote
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Description	Goat anti Mouse IgG (H/L):HRP

UniProt: P09104
Entrez Gene: ENO2
GO Terms: GO:0000015 phosphopyruvate hydratase complex; GO:0005886 plasma membrane; GO:0000287 magnesium ion binding; GO:0004634 phosphopyruvate hydratase activity; GO:0006094 gluconeogenesis; GO:0006096 glycolysis