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NCF2 antibody | H05/3G6

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Anti NCF2 Antibody, clone H05/3G6 (PrecisionAb Monoclonal Antibody) thumbnail image 1 Anti NCF2 Antibody, clone H05/3G6 (PrecisionAb Monoclonal Antibody) thumbnail image 2
Anti NCF2 Antibody, clone H05/3G6 (PrecisionAb Monoclonal Antibody) gallery image 1
Western blot analysis of whole cell lysates probed with NCF2 antibody (1/1,000, VMA00936) followed by detection with HRP conjugated Goat anti Mouse detection reagent (1/10,000, STAR207P) and visualized on the ChemiDoc MP. Arrow points to NCF2 (molecular weight 67 kDa)
Anti NCF2 Antibody, clone H05/3G6 (PrecisionAb Monoclonal Antibody) gallery image 2
Immunoprecipitation of NCF2 from RAW264.7 whole cell lysate. The Input lane is 10% input; the NCF2-IP lane is the immunoprecipitation with the NCF2 antibody (2.5 µg, VMA00936); the IgG-IP lane is the immunoprecipitation with Mouse monoclonal IgG (2.5 µg, MCA928). Western blot analysis was performed using NCF2 antibody (1/1000, VMA00936) followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000, 1705047) and visualized on the ChemiDoc MP. Arrow points to NCF2.

Mouse anti NCF2

Product Type
PrecisionAb Monoclonal
Clone
H05/3G6
Isotype
IgG1
Format
Purified
Specificity
NCF2

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Product Code Applications Pack Size List Price Your Price Qty
VMA00936
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
IP WB 100 µl loader
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loader

Mouse anti NCF2 antibody recognizes neutrophil cytosol factor 2, also known as NOXA2 and P67PHOX.

NCF2 is a cytosolic component of NADPH oxidase, necessary for phagocyte reactive oxygen species (ROS) production, phagocytic microbicidal activity and innate immunity (Zhang et al. 2018). NCF2 is upregulated in response to p53 induction, and NCF2 knockdown results in reduced production of ROS and stimulates cell death (Italiano et al. 2012). A variant of the gene encoding NCF2 causes altered protein binding and is associated with very early inflammatory bowel disease (Muise et al. 2012). Mutations in NCF2 can result in granulomatous disease, an immunodeficiency characterized by recurrent infections (Tarazona-Santos et al. 2013). Pattern of NCF2 expression has been suggested as a potential diagnostic tool in cervical cancer (Lomnytska et al. 2011).		 PrecisionAb Antibodies

Target Species
Human
Western Blotting
Mouse anti NCF2 detects a band of approximately 67 kDa in RAW264.7 cell lysates
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - Liquid
Preparation
Mouse monoclonal antibody affinity purified on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide
Immunogen
E. coli-derived recombinant protein of amino acids 1-526 of human NCF2
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunised BALB/c mice were fused with cells of the mouse SP2/0 myeloma cell line
Regulatory
For research purposes only
Guarantee
12 months from date of despatch
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories

Store undiluted at -20°C, avoiding repeated freeze thaw cycles

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunoprecipitation
Western Blotting 1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Description Product Code Applications Pack Size List Price Your Price Quantity
Goat anti Mouse IgG (H/L):HRP STAR207P WB 2 ml loader
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loader
Description Goat anti Mouse IgG (H/L):HRP

UniProt
P19878
Entrez Gene
NCF2
GO Terms
GO:0009055 electron carrier activity
GO:0005737 cytoplasm
GO:0005730 nucleolus
GO:0006968 cellular defense response
GO:0008022 protein C-terminus binding
GO:0042554 superoxide anion generation
GO:0043020 NADPH oxidase complex
GO:0045087 innate immune response
GO:0045730 respiratory burst

VMA00936

100005658

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