METAP2 antibody | OTI1F6
Mouse anti METAP2
METAP2 antibody recognizes methionine aminopeptidase 2, also known as eIF2-associated p67 homolog or initiation factor 2-associated 67 kDa glycoprotein. This antibody has been extensively validated for western blotting using whole cell lysates.
- Product Type
- PrecisionAb™ Monoclonal
- Clone
- OTI1F6
- Isotype
- IgG1
- Format
- Purified
| Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|
| VMA00197 | WB | 100 µl |
|
Mouse anti Human METAP2 antibody recognizes METAP2, also known as MAP 2, eIF-2-associated p67 homolog, initiation factor 2-associated 67 kDa glycoprotein, metAP 2, methionine aminopeptidase 2, p67, p67eIF2 and peptidase M 2.
METAP2 is a member of the methionyl aminopeptidase family and is a protein that binds 2 cobalt or manganese ions. This protein functions both by protecting the alpha subunit of eukaryotic initiation factor 2 from inhibitory phosphorylation and by removing the amino-terminal methionine residue from nascent protein. Increased expression of METAP2 is associated with various forms of cancer and the anti-cancer drugs fumagillin and ovalicin inhibit the protein by irreversibly binding to its active site. A pseudogene of METAP2 is located on chromosome 2 (provided by RefSeq, Jul 2008).
Mouse anti Human METAP2 antibody detects a band of 63 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
METAP2 is a member of the methionyl aminopeptidase family and is a protein that binds 2 cobalt or manganese ions. This protein functions both by protecting the alpha subunit of eukaryotic initiation factor 2 from inhibitory phosphorylation and by removing the amino-terminal methionine residue from nascent protein. Increased expression of METAP2 is associated with various forms of cancer and the anti-cancer drugs fumagillin and ovalicin inhibit the protein by irreversibly binding to its active site. A pseudogene of METAP2 is located on chromosome 2 (provided by RefSeq, Jul 2008).
Mouse anti Human METAP2 antibody detects a band of 63 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
Product Details
- Target Species
- Human
- Western Blotting
- Anti METAP2 detects a band of approximately 63 kDa in K562 cell lysates.
- Product Form
- Purified IgG - liquid
- Preparation
- Mouse monoclonal antibody purified by affinity chromatography from ascites.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
50% Glycerol - Immunogen
- Recombinant protein corresponding to amino acids 2-212 of human METAP2 (NP_006829) produced in E. coli
- Instructions For Use
- Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
Storage Information
- Storage
- Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
- Guarantee
- As supplied, 12 months from date of despatch.
More Information
- UniProt
- P50579 Related reagents
- Entrez Gene
- METAP2 Related reagents
- GO Terms
- GO:0004177 aminopeptidase activity
- GO:0008235 metalloexopeptidase activity
- GO:0046872 metal ion binding
- GO:0005737 cytoplasm
- GO:0006508 proteolysis
- GO:0016485 protein processing
- GO:0018206 peptidyl-methionine modification
- GO:0031365 N-terminal protein amino acid modification
- Acknowledgements
- PrecisionAb™ is a trademark of Bio-Rad Laboratories.
- Regulatory
- For research purposes only
Applications of METAP2 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Western Blotting | 1/1000 |
PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
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Secondary Antibodies Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Goat anti Mouse IgG (H/L):HRP | STAR207P | WB | 2 ml |
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