METAP2 antibody | OTI1F6

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Anti METAP2 Antibody, clone OTI1F6 (PrecisionAb™ Monoclonal Antibody) gallery image 1 — Western blot analysis of whole cell lysates probed with METAP2 antibody (**VMA00197**) followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000, STAR207P) and visualized on the ChemiDoc™ MP with 32 second exposure. Arrow points to METAP2 (molecular weight 63 kDa).

Mouse anti METAP2

METAP2 antibody recognizes methionine aminopeptidase 2, also known as eIF2-associated p67 homolog or initiation factor 2-associated 67 kDa glycoprotein. This antibody has been extensively validated for western blotting using whole cell lysates.

METAP2 Antibody with Control Lysate

Product Type: PrecisionAb™ Monoclonal
Clone: OTI1F6
Isotype: IgG1
Format: Purified

Product Code	Applications	Pack Size
VMA00197	WB	100 µl
VMA00197KT	WB	2 Westerns
With Lysate		400µg

Summary
Secondary Antibodies

Mouse anti Human METAP2 antibody recognizes METAP2, also known as MAP 2, eIF-2-associated p67 homolog, initiation factor 2-associated 67 kDa glycoprotein, metAP 2, methionine aminopeptidase 2, p67, p67eIF2 and peptidase M 2.

METAP2 is a member of the methionyl aminopeptidase family and is a protein that binds 2 cobalt or manganese ions. This protein functions both by protecting the alpha subunit of eukaryotic initiation factor 2 from inhibitory phosphorylation and by removing the amino-terminal methionine residue from nascent protein. Increased expression of METAP2 is associated with various forms of cancer and the anti-cancer drugs fumagillin and ovalicin inhibit the protein by irreversibly binding to its active site. A pseudogene of METAP2 is located on chromosome 2 (provided by RefSeq, Jul 2008).

Mouse anti Human METAP2 antibody detects a band of 63 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species: Human
Western Blotting: Anti METAP2 detects a band of approximately 63 kDa in K562 cell lysates.
Product Form: Purified IgG - liquid
Product Form: Purified IgG - liquid
Preparation: Mouse monoclonal antibody purified by affinity chromatography from ascites.
Preparation: 20μl Mouse monoclonal antibody purified by affinity chromatography from ascites.
Buffer Solution: Phosphate buffered saline
Buffer Solution: Phosphate buffered saline
Preservative Stabilisers: 0.09% Sodium Azide (NaN₃)
1% Bovine Serum Albumin
50% Glycerol
Preservative Stabilisers: 0.09% Sodium Azide (NaN₃)
1% Bovine Serum Albumin
50% Glycerol
Immunogen: Recombinant protein corresponding to amino acids 2-212 of human METAP2 (NP_006829) produced in E. coli
Instructions For Use: Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.

Positive Control Lysate

Lysate Composition: 400μg K562 lysate lyophilized in RIPA buffer.
Lysate Reconstitution: - If using DDT reconstitute the lyophilized lysate with 190μl DI H₂O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
- If using BME reconstitute the lyophilized lysate with 180μl DI H₂O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.

Storage Information

Storage: Store undiluted at -20^oC, avoiding repeated freeze thaw cycles.
Storage: Antibody: Store undiluted at -20^oC, avoiding repeated freeze thaw cycles.

Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
Shelf Life: As supplied, 12 months from date of despatch.
Shelf Life: As supplied, 12 months from date of despatch.

More Information

UniProt: P50579 Related reagents
Entrez Gene: METAP2 Related reagents
GO Terms: GO:0004177 aminopeptidase activity; GO:0008235 metalloexopeptidase activity; GO:0046872 metal ion binding; GO:0005737 cytoplasm; GO:0006508 proteolysis; GO:0016485 protein processing; GO:0018206 peptidyl-methionine modification; GO:0031365 N-terminal protein amino acid modification
Acknowledgements: PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Acknowledgements: PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Regulatory: For research purposes only

Applications of METAP2 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Western Blotting		1/1000
Western Blotting		1/1000

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

Secondary Antibodies Available

Description	Product Code	Pack Size	Applications	List Price	Quantity
Goat anti Mouse IgG (H/L):HRP	STAR207P	2 ml	WB
Goat anti Mouse IgG (H/L):HRP	STAR207P	2 ml	WB

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