Mcl-1 antibody

Mouse anti Mcl-1

Product Type
PrecisionAb Monoclonal
Isotype
IgG1
Format
Purified
Specificity
Mcl-1

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VMA00507
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
WB 100 µl loader
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Mouse anti Human Mcl-1 antibody recognizes the induced myeloid leukemia cell differentiation protein Mcl-1, also known as bcl-2-like protein 3, bcl-2-related protein EAT/mcl1, myeloid cell leukemia ES or myeloid cell leukemia sequence 1 (BCL2-related).

Mcl-1 is a member of the Bcl-2 family. Alternative splicing results in multiple transcript variants. The longest gene product (isoform 1) enhances cell survival by inhibiting apoptosis while the alternatively spliced shorter gene products (isoform 2 and isoform 3) promote apoptosis and are death-inducing (provided by RefSeq, Oct 2010).

Mouse anti Human Mcl-1 antibody detects a band of 40 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
PrecisionAb Antibodies

Target Species
Human
Western Blotting
Anti Mcl-1 detects a band of approximately 40 kDa in HeLa cell lysate.
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from ascites.
Buffer Solution
Phosphate buffered saline.
Preservative Stabilisers
0.09% Sodium Azide (NaN3).
Immunogen
E. coli derived recombinant protein corresponding to amino acids 1-230 of human Mcl-1
Regulatory
For research purposes only.
Guarantee
12 months from date of despatch.
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories.

Store undiluted at -20°C, avoiding repeated freeze thaw cycles.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000
The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

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UniProt
Q07820
Entrez Gene
MCL1
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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