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MAPK8 / JNK1 antibody | GH05/4G10

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Anti MAPK8 / JNK1 Antibody, clone GH05/4G10 (PrecisionAb Monoclonal Antibody) thumbnail image 1 Anti MAPK8 / JNK1 Antibody, clone GH05/4G10 (PrecisionAb Monoclonal Antibody) thumbnail image 2 Anti MAPK8 / JNK1 Antibody, clone GH05/4G10 (PrecisionAb Monoclonal Antibody) thumbnail image 3
Anti MAPK8 / JNK1 Antibody, clone GH05/4G10 (PrecisionAb Monoclonal Antibody) gallery image 1
Western blot analysis of whole cell lysates probed with Mouse anti Human MAPK8 / JNK1 antibody (VMA00407) followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000, STAR207P) and visualized on the ChemicDoc MP with 77 second exposure. Arrow points to MAPK8 / JNK1 (molecular weight 44/52 kDa).
Anti MAPK8 / JNK1 Antibody, clone GH05/4G10 (PrecisionAb Monoclonal Antibody) gallery image 2
Western blot analysis of whole cell lysates prepared from HEK293 separately transfected with two unique siRNA duplexes against MAPK8 / JKN1 (1 & 2) or a scrambled siRNA (-) probed with Mouse anti MAPK8/JKN1 antibody (VMA00407) followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000 STAR207P) and hFAB rhodamine anti-tubulin primary antibody (12004165) and visualized on the ChemiDoc MP with an 300 second (chemiluminescent channel) and 60 second (rhodamine channel) exposure.
Anti MAPK8 / JNK1 Antibody, clone GH05/4G10 (PrecisionAb Monoclonal Antibody) gallery image 3
Normalized signal intensity of MAPK8/ JNK1 from whole cell lysates prepared from HEK293 separately transfected with two unique siRNA duplexes against MAPK8/ JNK1 (1 & 2) or a scrambled siRNA (-). Signal intensity was normalized to the tubulin signal.

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WB

Mouse anti MAPK8 / JNK1

Product Type
PrecisionAb Monoclonal
Clone
GH05/4G10
Isotype
IgG1
Format
Purified
Specificity
MAPK8 / JNK1

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Product Code Applications Pack Size List Price Your Price Qty
VMA00407
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
WB 100 µl loader
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loader

Mouse anti Human MAPK8 / JNK1 antibody recognizes mitogen-activated protein kinase 8, also known as JUN N-terminal kinase, c-Jun N-terminal kinase 1, mitogen-activated protein kinase 8 isoform JNK1 alpha1, mitogen-activated protein kinase 8 isoform JNK1 beta2 and stress-activated protein kinase 1c.

Encoded by the MAPK8 / JNK1 gene, mitogen-activated protein kinase 8 is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. This kinase is activated by various cell stimuli, and targets specific transcription factors, and thus mediates immediate-early gene expression in response to cell stimuli. The activation of this kinase by tumor-necrosis factor alpha (TNF-alpha) is found to be required for TNF-alpha induced apoptosis. This kinase is also involved in UV radiation induced apoptosis, which is thought to be related to cytochrome c-mediated cell death pathway. The mouse counterpart of MAPK8 suggested that this kinase plays a key role in T cell proliferation, apoptosis and differentiation. Five alternatively spliced transcript variants encoding distinct isoforms have been reported (provided by RefSeq, Jun 2013).

Mouse anti Human MAPK8 / JNK1 antibody detects a band of 44/52 kDa. The antibody has been extensively validated for western blotting using whole cell lysates. 		PrecisionAb Antibodies

Target Species
Human
Western Blotting
Anti MAPK8 / JNK1 detects a band of approximately 44/52 kDa in K562 cell lysates.
Species Cross-Reactivity
Target SpeciesCross Reactivity
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Mouse monoclonal antibody purified by affinity chromatography on Protein G from tissue culture supernatant.
Buffer Solution
Phosphate buffered saline.
Preservative Stabilisers
0.09% Sodium Azide (NaN3).
Immunogen
E.coli-derived recombinant protein corresponding to aa 1-427 of human MAPK8 / JNK1
Approx. Protein Concentrations
IgG concentration 0.5 mg/ml
Regulatory
For research purposes only.
Guarantee
12 months from date of despatch.
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories.

Store undiluted at -20°C, avoiding repeated freeze thaw cycles.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000
The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Description Product Code Applications Pack Size List Price Your Price Quantity
Goat anti Mouse IgG (H/L):HRP STAR207P WB 2 ml loader
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loader
Description Goat anti Mouse IgG (H/L):HRP

UniProt
P45983
Entrez Gene
MAPK8
GO Terms
GO:0005515 protein binding
GO:0002755 MyD88-dependent toll-like receptor signaling pathway
GO:0002756 MyD88-independent toll-like receptor signaling pathway
GO:0005829 cytosol
GO:0004705 JUN kinase activity
GO:0005524 ATP binding
GO:0005654 nucleoplasm
GO:0008063 Toll signaling pathway
GO:0008629 induction of apoptosis by intracellular signals
GO:0008633 activation of pro-apoptotic gene products
GO:0009411 response to UV
GO:0034130 toll-like receptor 1 signaling pathway
GO:0034134 toll-like receptor 2 signaling pathway
GO:0034138 toll-like receptor 3 signaling pathway
GO:0034142 toll-like receptor 4 signaling pathway
GO:0043066 negative regulation of apoptosis
GO:0045087 innate immune response
GO:0048011 nerve growth factor receptor signaling pathway
GO:0051090 regulation of transcription factor activity
GO:0051403 stress-activated MAPK cascade

VMA00407

160303

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