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MAP4K1 antibody | C01/4C3

Mouse anti Human MAP4K1

Product Type
PrecisionAb Monoclonal

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SDS Safety Datasheet SDS
IP WB 100 µl loader
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Mouse anti Human MAP4K1 antibody recognizes mitogen-activated protein kinase kinase kinase kinase 1, also known as hematopoietic progenitor kinase 1 (HPK1).

MAP4K1 has been shown to play a role in the activation of c-Jun N-terminal kinases (JNKs) (Chuang et al. 2016) and IKK-alpha/beta. MAP4K1 supports apoptosis of T cells through inhibition of the anti-apoptotic action of NF-κB and induction of activity of JNK and OHPK1 (Schulze-Luehrmann et al. 2016).
PrecisionAb Antibodies

Target Species
Western Blotting
Mouse anti Human MAP4K1 antibody detects a band of 96 kDa in Jurkat cell lysates
Product Form
Purified IgG - liquid
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
E. coli-derived recombinant protein, aa 1-274 of human MAP4K1
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
For research purposes only
12 months from date of despatch
PrecisionAb is a trademark of Bio-Rad Laboratories

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunoprecipitation 2.5 µg
Western Blotting 1/2000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Entrez Gene
GO Terms
GO:0000185 activation of MAPKKK activity
GO:0005515 protein binding
GO:0005083 small GTPase regulator activity
GO:0005524 ATP binding
GO:0007257 activation of JUN kinase activity
GO:0008349 MAP kinase kinase kinase kinase activity
GO:0018105 peptidyl-serine phosphorylation



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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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