MAP1LC3B antibody | RM293

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Rabbit anti MAP1LC3B

Product Type
Monoclonal Antibody
Clone
RM293
Isotype
IgG
Product Code Applications Pack Size List Price Quantity
MCA6341
Datasheet
IC P WB
SDS
0.1 ml loader

Rabbit anti MAP1LC3B antibody recognizes microtubule-associated proteins 1A/1B light chain 3B, also known as autophagy-related protein LC3B.

MAP1LC3B is a key protein in autophagy and is essential for autophagosome elongation (Liu et al. 2018). During autophagy, the carboxy terminus of MAP1LC3B is cleaved to produce LC3-I in the cytoplasm. LC3-I is then lipidated, resulting in LC3-II which binds to autophagic vesicles. Presence of LC3-II in autophagosomes is an indicator of autophagy. Therefore, changes in the location and abundance of these proteins can be used to study autophagy (Tang et al. 2019). MAP1LC3B has been found to be activated in solid tumors and is associated with tumor progression (Kang et al. 2019).

Product Details

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse Expected from Sequence
Rat Expected from Sequence
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A from animal origin-free culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide
1% Bovine Serum Albumin
50% Glycerol
Immunogen
A peptide corresponding to the N-terminus of human MAP1LC3

Storage Information

Storage
Store at -20°C only.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
12 months from date of despatch

More Information

UniProt
Q9GZQ8
Entrez Gene
MAP1LC3B
GO Terms
GO:0005515 protein binding
GO:0000421 autophagic vacuole membrane
GO:0005874 microtubule
GO:0006914 autophagy
GO:0012505 endomembrane system
GO:0031410 cytoplasmic vesicle
Regulatory
For research purposes only

Applications of MAP1LC3B antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunocytochemistry 1/100 1/200
Immunohistology - Paraffin 1/100 1/200
Western Blotting 1/100 1/200
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Rabbit IgG (Fc):Biotin STAR121B E WB 1 mg loader
Goat anti Rabbit IgG (Fc):FITC STAR121F F 1 mg loader
Goat anti Rabbit IgG (Fc):HRP STAR121P E WB 1 mg loader
Goat anti Rabbit IgG (H/L):HRP STAR124P C E WB 1 mg loader
Sheep anti Rabbit IgG:FITC STAR34B C F 1 mg loader
Sheep anti Rabbit IgG:RPE STAR35A F 1 ml loader
Sheep anti Rabbit IgG:DyLight®488 STAR36D488GA F IF WB 0.1 mg
Sheep anti Rabbit IgG:DyLight®680 STAR36D680GA F IF WB 0.1 mg
Sheep anti Rabbit IgG:Dylight®800 STAR36D800GA F IF WB 0.1 mg

Fluorescent Spectraviewer

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