Rabbit anti MAP1LC3B antibody
recognizes microtubule-associated proteins 1A/1B light chain 3B, also known as autophagy-related protein LC3B.
MAP1LC3B is a key protein in autophagy and is essential for autophagosome elongation (Liu et al. 2018
). During autophagy, the carboxy terminus of MAP1LC3B is cleaved to produce LC3-I in the cytoplasm. LC3-I is then lipidated, resulting in LC3-II which binds to autophagic vesicles. Presence of LC3-II in autophagosomes is an indicator of autophagy. Therefore, changes in the location and abundance of these proteins can be used to study autophagy (Tang et al. 2019
). MAP1LC3B has been found to be activated in solid tumors and is associated with tumor progression (Kang et al. 2019
- Target Species
- Species Cross-Reactivity
|Target Species||Cross Reactivity|
||Expected from Sequence
||Expected from Sequence
- N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Purified IgG prepared by affinity chromatography on Protein A from animal origin-free culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide
1% Bovine Serum Albumin
- A peptide corresponding to the N-terminus of human MAP1LC3
- Store at -20°C only.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.
- 12 months from date of despatch
- Entrez Gene
- GO Terms
autophagic vacuole membrane
- For research purposes only
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Applications of MAP1LC3B antibody
|Immunohistology - Paraffin
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Copyright © 2021 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
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