MALT1 antibody | 50

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Mouse anti MALT1

Product Type: PrecisionAb Monoclonal
Clone: 50
Isotype: IgG1
Format: Purified
Specificity: MALT1

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Product Code Applications Pack Size List Price Your Price Qty

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VMA00038 Datasheet SDS SDS	IP P * WB	100 µl		Log in
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siRNA Validated

IP-MS Validated

Anti MALT1 Antibody, clone 50 (PrecisionAb Monoclonal Antibody) thumbnail image 1

Anti MALT1 Antibody, clone 50 (PrecisionAb Monoclonal Antibody) thumbnail image 2

Anti MALT1 Antibody, clone 50 (PrecisionAb Monoclonal Antibody) thumbnail image 3

Anti MALT1 Antibody, clone 50 (PrecisionAb Monoclonal Antibody) thumbnail image 4

Anti MALT1 Antibody, clone 50 (PrecisionAb Monoclonal Antibody) thumbnail image 5

Anti MALT1 Antibody, clone 50 (PrecisionAb Monoclonal Antibody) gallery image 1 — Stainig of paraffin embedded human tonsil stained with Mouse anti Human MALT1 (**VMA00038**).

Anti MALT1 Antibody, clone 50 (PrecisionAb Monoclonal Antibody) gallery image 2 — Immunoprecipitation of MALT1 from HEK293 whole cell lysate. The Input lane is 10% input; the MALT1-IP lane is the immunoprecipitation with the MALT1 antibody (2.5 µg, **VMA00038**); the IgG-IP lane is the immunoprecipitation with Mouse monoclonal IgG (2.5 µg,MCA928). Western blot analysis was performed using MALT1 antibody (1/2,000, **VMA00038**) followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000, STAR207P) and visualized on the ChemiDoc MP. Arrow points to MALT1.

Anti MALT1 Antibody, clone 50 (PrecisionAb Monoclonal Antibody) gallery image 3 — Western blot analysis of whole cell lysates probed with MALT1 antibody (**VMA00038**) followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000, STAR207P) and visualized on the ChemiDoc MP with 200 second exposure. Arrow points to MALT1 (molecular weight 92 kDa).

Anti MALT1 Antibody, clone 50 (PrecisionAb Monoclonal Antibody) gallery image 4 — Western blot analysis of whole cell lysates prepared from HEK293 separately transfected with two unique siRNA duplexes against MALT1 (1 & 2) or a scrambled siRNA (-) probed with Mouse anti MALT1 antibody (**VMA00038**) followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000 1705047) and hFAB rhodamine anti-tubulin primary antibody (12004166) and visualized on the ChemiDoc MP with a 60 second (chemiluminescent channel) and 60 second (rhodamine channel) exposure.

Anti MALT1 Antibody, clone 50 (PrecisionAb Monoclonal Antibody) gallery image 5 — Normalized signal intensity of MALT1 from whole cell lysates prepared from HEK293 separately transfected with two unique siRNA duplexes against MALT1 (1 & 2) or a scrambled siRNA (-). Signal intensity was normalized to the tubulin signal.

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P IP WB

Mouse anti Human MALT1 antibody recognizes human mucosal-associated lymphoid tissue lymphoma translocation 1 (MALT1), also known as MALT lymphoma-associated translocation and paracaspase. MALT1 is an 824 amino acid protein belonging to the peptidase C14B family containing a single death domain and two Ig-like C2-type domains. In normal lymphocytes, MALT1 plays an important role in antigen receptor-mediated lymphocyte activation. In T-cells MALT1 is recruited by activated CARMA1, along with Bcl-10, to form a CARMA1-Bcl10-MALT1 (CBM) complex which is involved in the activation of NF-kappaB (Yang et al. 2013).

MALT1 was initially identified as a recurrent translocation t(11;18)(q21:q21) in mucosal-associated lymphomas (Horsman et al. 1992), which creates a functional fusion oncoprotein consisting of MALT1 and apoptosis inhibitor API2 (Dierlamm et al. 1999). Overexpression of MALT1, or expression of the API2-MALT1 fusion protein, leads to the uncontrolled activation of NF-kappaB which is stimulus-independent (Zhou et al. 2005). Mouse anti Human MALT1 recognizes both MALT1 and the MALT1-API2 fusion protein (Murga Penas et al. 2007). The MALT1-API2 oncoprotein plays a role in non-canonical activation of NF-κb through NIK cleavage (Rosebeck et al.2011).

Mouse anti Human MALT1 antibody recognizes human MALT1 as a single band of ~92 kDa in multiple human cell line lysates by western blotting under reducing conditions.

Target Species: Human
Western Blotting: Anti MALT1 antibody detects a band of approximately 92 kDa in HEK293 cell lysates.
Product Form: Purified IgG - liquid
Preparation: Mouse monoclonal antibody purified by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution: Phosphate buffered saline
Preservative Stabilisers: 0.09% Sodium Azide (NaN₃)
Immunogen: Recombinant protein corresponding to amino acids 701-808 located within the C-terminus of human MALT1
Approx. Protein Concentrations: IgG concentration 0.5 mg/ml
Regulatory: For research purposes only
Guarantee: 12 months from date of despatch
Acknowledgements: PrecisionAb is a trademark of Bio-Rad Laboratories.

This product is shipped at ambient temperature.
Store undiluted at -20^oC, avoiding repeated freeze thaw cycles.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Immunohistology - Paraffin ¹
Immunoprecipitation
Western Blotting			1/1000

¹This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Learn about how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Description	Product Code	Applications	Pack Size	Your Price
Goat anti Mouse IgG (H/L):HRP	STAR207P	WB	2 ml	Log in
List Price	Your Price
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Description	Goat anti Mouse IgG (H/L):HRP

References for MALT1 antibody

Ye, H. et al. (2005) MALT lymphoma with t(14;18)(q32;q21)/IGH-MALT1 is characterized by strong cytoplasmic MALT1 and BCL10 expression.
J Pathol. 205 (3): 293-301.

UniProt: Q9UDY8
Entrez Gene: MALT1
GO Terms: GO:0004197 cysteine-type endopeptidase activity; GO:0002726 positive regulation of T cell cytokine production; GO:0005829 cytosol; GO:0004842 ubiquitin-protein ligase activity; GO:0004871 signal transducer activity; GO:0005634 nucleus; GO:0006508 proteolysis; GO:0006916 anti-apoptosis; GO:0007250 activation of NF-kappaB-inducing kinase activity; View More GO Terms; GO:0032449 CBM complex; GO:0032743 positive regulation of interleukin-2 production; GO:0043123 positive regulation of I-kappaB kinase/NF-kappaB cascade; GO:0043621 protein self-association; GO:0048471 perinuclear region of cytoplasm; GO:0050852 T cell receptor signaling pathway; GO:0050870 positive regulation of T cell activation; GO:0051168 nuclear export; GO:0051259 protein oligomerization