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Lrpprc antibody | AB02/2F11

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siRNA Validated
IP-MS Validated
Anti Lrpprc Antibody, clone AB02/2F11 (PrecisionAb Monoclonal Antibody) thumbnail image 1 Anti Lrpprc Antibody, clone AB02/2F11 (PrecisionAb Monoclonal Antibody) thumbnail image 2 Anti Lrpprc Antibody, clone AB02/2F11 (PrecisionAb Monoclonal Antibody) thumbnail image 3 Anti Lrpprc Antibody, clone AB02/2F11 (PrecisionAb Monoclonal Antibody) thumbnail image 4
Anti Lrpprc Antibody, clone AB02/2F11 (PrecisionAb Monoclonal Antibody) gallery image 1
Western blot analysis of whole cell lysates probed with LRPPRC antibody (1/10000, VMA00967) followed by detection with HRP conjugated Goat anti Mouse IgG (1/50000, STAR207P) and visualized on the ChemiDoc MP. Arrow points to LRPPRC (molecular weight 146 kDa)
Anti Lrpprc Antibody, clone AB02/2F11 (PrecisionAb Monoclonal Antibody) gallery image 2
Immunoprecipitation of LRPPRC from K562 whole cell lysate. The Input lane is 10% input; the LRPPRC-IP lane is the immunoprecipitation with the LRPPRC antibody (2.5 µg, VMA00967); the IgG-IP lane is the immunoprecipitation with Mouse monoclonal IgG (2.5 µg, MCA928). Western blot analysis was performed using LRPPRC antibody (1/1000, VMA00967) followed by detection with HRP conjugated Goat anti Mouse IgG (1/50,000, 1705047) and visualized on the ChemiDoc MP. Arrow points to LRPPRC.
Anti Lrpprc Antibody, clone AB02/2F11 (PrecisionAb Monoclonal Antibody) gallery image 3
Western blot analysis of whole cell lysates prepared from HepG2 separately transfected with two unique siRNA duplexes against LRPPRC (1 & 2) or a scrambled siRNA (-) probed with Mouse anti LRPPRC antibody (VMA00967) followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000 STAR207P) and hFAB rhodamine anti-tubulin primary antibody 12004165) and visualized on the ChemiDoc MP with an 31 second (chemiluminescent channel) and 30 second (rhodamine channel) exposure.
Anti Lrpprc Antibody, clone AB02/2F11 (PrecisionAb Monoclonal Antibody) gallery image 4
Normalized signal intensity of LRPPRC from whole cell lysates prepared from HepG2 separately transfected with two unique siRNA duplexes against LRPPRC (1 & 2) or a scrambled siRNA (-). Signal intensity was normalized to the tubulin signal.

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WB IP

Mouse anti Lrpprc

Product Type
PrecisionAb Monoclonal
Clone
AB02/2F11
Isotype
IgG1
Format
Purified
Specificity
Lrpprc

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Product Code Applications Pack Size List Price Your Price Qty
VMA00967
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SDS Safety Datasheet SDS
IP WB 100 µl loader
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Mouse anti LRPPRC antibody recognizes leucine-rich PPR motif-containing protein, mitochondrial, also known as LRP130. LRPPRC is a member of the pentatricopeptide repeat (PPR) family, a group of proteins which play major roles in RNA stability, regulation, processing, splicing, translation, and editing. LRPPRC is a multifunctional protein involved with mitochondrial gene expression and function, cell cycle progression, and tumorigenesis (Li et al. 2014). It can be found localized to the nuclear membrane, nucleoplasm, endoplasmic reticulum, cytoskeleton, and mitochondria, and carries out its roles by direct or indirect interaction with other proteins (Cui et al. 2019). LRPPRC has been found to be overexpressed in a variety of human cancers including lung adenocarcinoma, oesophageal squamous cell carcinoma, stomach, colon, mammary and endometrial adenocarcinoma, and lymphoma. These findings are consistent with observations that LRPPRC can suppress autophagy and mitophagy (Jiang et al. 2014). PrecisionAb Antibodies

Target Species
Human
Western Blotting
Mouse anti LRPPRC detects a band of approximately 146 kDa in K562 cell lysates
Product Form
Purified IgG - Liquid
Preparation
Mouse monoclonal antibody affinity purified on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide
Immunogen
E. coli-derived recombinant protein of amino acids 611-907 of human LRPPRC
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunised BALB/c mice were fused with cells of the mouse SP2/0 myeloma cell line
Regulatory
For research purposes only
Guarantee
12 months from date of despatch
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories

Store undiluted at -20°C, avoiding repeated freeze thaw cycles

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunoprecipitation
Western Blotting 1/10000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Description Product Code Applications Pack Size List Price Your Price Quantity
Goat anti Mouse IgG (H/L):HRP STAR207P WB 2 ml
List Price Your Price
Description Goat anti Mouse IgG (H/L):HRP

UniProt
P42704
Entrez Gene
LRPPRC
GO Terms
GO:0000794 condensed nuclear chromosome
GO:0003723 RNA binding
GO:0005637 nuclear inner membrane
GO:0005640 nuclear outer membrane
GO:0005654 nucleoplasm
GO:0005856 cytoskeleton
GO:0006350 transcription
GO:0008017 microtubule binding
GO:0042645 mitochondrial nucleoid
GO:0045449 regulation of transcription
GO:0047497 mitochondrion transport along microtubule
GO:0048471 perinuclear region of cytoplasm
GO:0048487 beta-tubulin binding
GO:0051028 mRNA transport

VMA00967

100005803

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