LMAN1 antibody | OTI2B10

Mouse anti LMAN1

Product Type
PrecisionAb Monoclonal
Clone
OTI2B10
Isotype
IgG1
Format
Purified
Specificity
LMAN1

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VMA00127
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Mouse anti Human LMAN1 antibody recognizes LMAN1, also known as ER-Golgi intermediate compartment 53 kDa protein, endoplasmic reticulum-golgi intermediate compartment protein 53, intracellular mannose specific lectin, intracellular mannose-specific lectin MR60 and protein ERGIC-53.

Encoded by the LMAN1 gene, this protein is a type I integral membrane protein localized in the intermediate region between the endoplasmic reticulum and the Golgi, presumably recycling between the two compartments. It is a mannose-specific lectin and is a member of a novel family of plant lectin homologs in the secretory pathway of animal cells. Mutations in the LMAN1 gene are associated with a coagulation defect. Using positional cloning, the gene was identified as the disease gene leading to combined factor V-factor VIII deficiency, a rare, autosomal recessive disorder in which both coagulation factors V and VIII are diminished (provided by RefSeq, Jul 2008).

Mouse anti Human LMAN1 antibody detects a band of 54 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
PrecisionAb Antibodies

Target Species
Human
Western Blotting
Anti LMAN1 detects a band of approximately 54 kDa in HEK293 cell lysates.
Product Form
Purified IgG - liquid
Preparation
Mouse monoclonal antibody purified by affinity chromatography from ascites.
Buffer Solution
Phosphate buffered saline.
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
50% Glycerol
Immunogen
Full length recombinant human LMAN1 (NP_005561) produced in HEK293T cells
Approx. Protein Concentrations
IgG concentration 0.5 mg/ml.
Regulatory
For research purposes only.
Guarantee
12 months from date of despatch.
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories.

Store undiluted at -20°C, avoiding repeated freeze thaw cycles.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000
The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

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Description Goat anti Mouse IgG (H/L):HRP

UniProt
P49257
Entrez Gene
LMAN1
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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