Lineage Cocktail | UCHT1 / TUK4 / DJ130c / LT19 / 2H7 / 123C3
Human Lineage Cocktail:Alexa Fluor® 700
- Product Type
- Monoclonal Antibody Panel
- Clone
- UCHT1 / TUK4 / DJ130c / LT19 / 2H7 / 123C3
- Isotype
- Cocktail
- Specificity
- Lineage Cocktail
Human Lineage Cocktail:Alexa Fluor® 700 is a cocktail of antibodies recognising all leucocytes in human peripheral blood, with the exception of dendritic cells and basophils. Dendritic cells in peripheral blood are characterised by the phenotype HLA-DR+ve, Lineage Cocktail -ve. Basophils are HLA-DR -ve. Human Lineage Cocktail:Alexa Fluor® 700 may therefore be used in combination with HLA-DR specific antibodies (e.g. MCA1879PE ) to identify peripheral blood dendritic cells. The individual antibodies in this cocktail are specific for cell subsets as follows:- CD3 T lymphocytes CD14 Monocytes CD16 NK cells, granulocytes CD19 B lymphocytes CD20 B lymphocytes CD56 NK cells |
- Target Species
- Human
- Product Form
- Purified IgG conjugated to Alexa Fluor® 700 - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide 1% Bovine Serum Albumin - Approx. Protein Concentrations
- MCA2692A700T: IgG concentration 0.05mg/ml
- MCA2692A700: IgG concentration 0.05 mg/ml
- Antibody Isotypes
- IgG1/IgG2a/IgG1/IgG1/IgG2b/IgG1
- Max Ex/Em
-
Fluorophore Excitation Max (nm) Emission Max (nm) Alexa Fluor®700 702 723 - Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
- Acknowledgements
- This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
Application Name | Verified | Min Dilution | Max Dilution |
---|---|---|---|
Flow Cytometry | Neat |
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
How to Use the Spectraviewer
Watch the Tool Tutorial Video ▸- Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
- Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
- Select the lasers and filters you wish to include
- Select combined or multi-laser view to visualize the spectra
Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
---|---|---|---|---|---|---|---|
Human Seroblock | BUF070A | F | 50 Test | Log in | |||
List Price | Your Price | ||||||
Log in | |||||||
Description | Human Seroblock | ||||||
Human Seroblock | BUF070B | F | 200 Test | Log in | |||
List Price | Your Price | ||||||
Log in | |||||||
Description | Human Seroblock |
References for Lineage Cocktail
-
Tjomsland, V. et al. (2010) Semi mature blood dendritic cells exist in patients with ductal pancreatic adenocarcinoma owing to inflammatory factors released from the tumor.
PloS One 5: e13441. -
Ramwadhdoebe, T.H. et al. (2019) Myeloid Dendritic Cells Are Enriched in Lymph Node Tissue of Early Rheumatoid Arthritis Patients but not in At Risk Individuals.
Cells. 8 (7): 756.
- RRID
- AB_915435
Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"
View all Anti-Human ProductsAlways be the first to know.
When we launch new products and resources to help you achieve more in the lab.
Yes, sign me up