Lineage Cocktail | UCHT1/TUK4/DJ130c/LT19/2H7/123C3

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Human Lineage Cocktail:Alexa Fluor® 700

Product Type
Monoclonal Antibody Panel
Clone
UCHT1/TUK4/DJ130c/LT19/2H7/123C3
Isotype
Cocktail
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
MCA2692A700T F 25 Tests/0.25ml
MCA2692A700 F 100 Tests/1ml
Human Lineage Cocktail:Alexa Fluor® 700 is a cocktail of antibodies recognising all leucocytes in human peripheral blood, with the exception of dendritic cells and basophils. Dendritic cells in peripheral blood are characterised by the phenotype HLA-DR+ve, Lineage Cocktail -ve. Basophils are HLA-DR -ve. Human Lineage Cocktail:Alexa Fluor® 700 may therefore be used in combination with HLA-DR specific antibodies (e.g. MCA1879PE ) to identify peripheral blood dendritic cells.

The individual antibodies in this cocktail are specific for cell subsets as follows:-
CD3 T lymphocytes
CD14 Monocytes
CD16 NK cells, granulocytes
CD19 B lymphocytes
CD20 B lymphocytes
CD56 NK cells

Product Details

Target Species
Human
Product Form
Purified IgG conjugated to Alexa Fluor® 700 - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
1%Bovine Serum Albumin
Approx. Protein Concentrations
Pack Size: 25 Tests/0.25ml
IgG concentration 0.05mg/ml
Pack Size: 100 Tests/1ml
IgG concentration 0.05 mg/ml
Antibody Isotypes
IgG1/IgG2a/IgG1/IgG1/IgG2b/IgG1

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.

More Information

Acknowledgements
This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
Regulatory
For research purposes only

Applications of Lineage Cocktail

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
Human Seroblock BUF070A 50 Test F
Human Seroblock BUF070B 200 Test F

Product Specific References

References for Lineage Cocktail

  1. Tjomsland, V. et al. (2010) Semi mature blood dendritic cells exist in patients with ductal pancreatic adenocarcinoma owing to inflammatory factors released from the tumor.
    PloS One 5: e13441.