Laminin Beta 3 antibody | OTI3A2

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Mouse anti Laminin Beta 3

Laminin beta 3 antibody recognizes laminin subunit beta-3, also known as epiligrin subunit bata, kalinin B1 chain, kalinin subunit beta, kalinin-140kDa, laminin B1k chain or laminin S B3 chain. This antibody has been extensively validated for western blotting using whole cell lysates.

Laminin Beta 3 Antibody with Control Lysate

Laminin beta 3 antibody recognizes laminin subunit beta-3, also known as epiligrin subunit bata, kalinin B1 chain, kalinin subunit beta, kalinin-140kDa, laminin B1k chain or laminin S B3 chain. This antibody has been extensively validated for western blotting using whole cell lysates.

Product Type
PrecisionAb™ Monoclonal
Clone
OTI3A2
Isotype
IgG2a
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
VMA00266 WB 100 µl
VMA00266KT WB 2 Westerns
With Lysate 400µg
Mouse anti Human laminin beta 3 antibody recognizes the laminin subunit beta-3, also known as epiligrin subunit bata, kalinin B1 chain, kalinin subunit beta, kalinin-140kDa, laminin B1k chain, laminin S B3 chain, laminin subunit beta-3, laminin, beta 3 (nicein (125kD), kalinin (140kD), BM600 (125kD)), laminin-5 subunit beta, nicein subunit beta and nicein-125kDa.

Encoded by LAMB3 gene, laminin beta 3 belongs to a family of basement membrane proteins. This protein, together with an alpha and a gamma subunit, forms laminin-5. Mutations in LAMB3 cause epidermolysis bullosa junctional Herlitz type, and generalized atrophic benign epidermolysis bullosa, diseases that are characterized by blistering of the skin. Multiple alternatively spliced transcript variants that encode the same protein have been found for LAMB3 (provided by RefSeq, Jul 2008).

Mouse anti Human laminin beta 3 antibody detects a band of 150 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species
Human
Product Form
Purified IgG - liquid
Product Form
Purified IgG - liquid
Preparation
Mouse monoclonal antibody purified by affinity chromatography from ascites
Preparation
20μl Mouse monoclonal antibody purified by affinity chromatography from ascites
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
50% Glycerol
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
50% Glycerol
Immunogen
Recombinant protein corresponding to amino acids 424-773 of human laminin beta 3 (NP_000219) produced in E. coli

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Storage
Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
Shelf Life
As supplied, 12 months from date of despatch.
Shelf Life
As supplied, 12 months from date of despatch.

More Information

UniProt
Q13751 Related reagents
Entrez Gene
LAMB3 Related reagents
GO Terms
GO:0007155 cell adhesion
GO:0005198 structural molecule activity
GO:0008544 epidermis development
GO:0031581 hemidesmosome assembly
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Regulatory
For research purposes only

Applications of Laminin Beta 3 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000
Western Blotting 1/1000

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
Western Blotting
Anti laminin beta 3 detects a band of approximately 150 kDa in A431 cell lysates
Western Blotting
Anti laminin beta 3 detects a band of approximately 150 kDa in A431 cell lysates
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Goat anti Mouse IgG (H/L):HRP STAR207P 2 ml WB
Goat anti Mouse IgG (H/L):HRP STAR207P 2 ml WB