Itch antibody

Mouse anti Itch

Product Type
PrecisionAb Monoclonal
Isotype
IgG2b
Format
Purified
Specificity
Itch

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VMA00405
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SDS Safety Datasheet SDS
WB 100 µl loader
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Mouse anti Human ITCH antibody recognizes ITCH, also known as E3 ligase Itchy homolog, NFE2-associated polypeptide 1, atrophin-1 interacting protein 4, AIP4 or dJ468O1.1 (atrophin 1 interacting protein 4 (AIP4)) and itchy E3 ubiquitin protein ligase homolog.

The ITCH gene encodes a member of the Nedd4 family of HECT domain E3 ubiquitin ligases. HECT domain E3 ubiquitin ligases transfer ubiquitin from E2 ubiquitin-conjugating enzymes to protein substrates, thus targeting specific proteins for lysosomal degradation. The encoded protein plays a role in multiple cellular processes including erythroid and lymphoid cell differentiation and the regulation of immune responses. Mutations in ITCH are a cause of syndromic multisystem autoimmune disease. Alternatively spliced transcript variants encoding multiple isoforms have been observed for ITCH (provided by RefSeq, Mar 2012).

Mouse anti Human ITCH antibody detects a band of 102 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
PrecisionAb Antibodies

Target Species
Human
Western Blotting
Anti ITCH detects a band of approximately 102 kDa in A431 cell lysates.
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Mouse monoclonal antibody purified by affinity chromatography on Protein G from ascites.
Buffer Solution
Phosphate buffered saline.
Preservative Stabilisers
0.09% Sodium Azide (NaN3).
Immunogen
Recombinant protein corresponding to aa 257-903 of human ITCH
Regulatory
For research purposes only.
Guarantee
12 months from date of despatch.
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories.

Store undiluted at -20°C, avoiding repeated freeze thaw cycles.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000
The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

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Goat anti Mouse IgG (H/L):HRP STAR207P WB 2 ml loader
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Description Goat anti Mouse IgG (H/L):HRP

UniProt
Q96J02
Entrez Gene
ITCH
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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