INI-1 antibody | E01/5G8

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Mouse anti INI-1

Product Type
PrecisionAb Monoclonal
Clone
E01/5G8
Isotype
IgG2a
Format
Purified
Product Code Applications Pack Size List Price Quantity
VMA00734
Datasheet
WB
MSDS
100 µl loader

Mouse anti Human INI-1 antibody recognizes INI-1, also known as RDT, SMARCB1, hSNF5, integrase interactor 1 protein and SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily B member 1.

INI-1 is a core subunit of the ATP-dependent SWI/SNF chromatin remodeling complex (Kohasi and Oda 2017) which regulates transcription, and is ubiquitously expressed in the nuclei of all normal cells. It is thought that INI-1 acts an an adaptor protein, recruiting the SWI/SNF complex to the site of transcription where it can regulate transcription (La Porte et al. 2016). The protein INI-1 is a potent tumor suppressor. Inactivation of the gene encoding INI-1 has been implicated in pathogenesis of a range of malignant neoplasms that have rhabdoid cytomorphology, defined as large cells with specific nuclear and cytoplasmic features (Bishop et al. 2014). In addition to INI-1, over 20 members of the SWI/SNF complex have been found to be altered in human tumors (Biegel et al. 2014). INI-1 incorporates into HIV-1 virons and plays important roles in HIV-1 transcription and particle production (La Porte et al. 2016).

Product Details

Target Species
Human
Western Blotting
Mouse anti INI-1 detects a band of approximately 44 kDa in Jurkat cell lysates
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - Liquid
Preparation
Mouse monoclonal antibody affinity purified on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide
Immunogen
E. coli--derived recombinant protein of amino acids 81-181 of human INI-1
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunised BALB/c mice were fused with cells of the mouse SP2/0 myeloma cell line

Storage Information

Storage
Store undiluted at -20°C, avoiding repeated freeze thaw cycles
Guarantee
12 months from date of despatch

More Information

UniProt
Q12824
Entrez Gene
SMARCB1
GO Terms
GO:0000228 nuclear chromosome
GO:0002039 p53 binding
GO:0005654 nucleoplasm
GO:0005730 nucleolus
GO:0006338 chromatin remodeling
GO:0006350 transcription
GO:0007049 cell cycle
GO:0007399 nervous system development
GO:0010552 positive regulation of gene-specific transcription from RNA polymerase II promoter
GO:0015074 DNA integration
GO:0016514 SWI/SNF complex
GO:0044419 interspecies interaction between organisms
GO:0045090 retroviral genome replication
GO:0051091 positive regulation of transcription factor activity
GO:0071564 npBAF complex
GO:0071565 nBAF complex
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories
Regulatory
For research purposes only

Applications of INI-1 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Mouse IgG (H/L):HRP STAR207P WB 2 ml