INI-1 antibody | E01/5G8
Mouse anti INI-1
- Product Type
- PrecisionAb Monoclonal
- Clone
- E01/5G8
- Isotype
- IgG2a
- Format
- Purified
- Specificity
- INI-1
Antibody Quality and Performance Guarantee
| Mouse anti Human INI-1 antibody recognizes INI-1, also known as RDT, SMARCB1, hSNF5, integrase interactor 1 protein and SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily B member 1. INI-1 is a core subunit of the ATP-dependent SWI/SNF chromatin remodeling complex (Kohasi and Oda 2017) which regulates transcription, and is ubiquitously expressed in the nuclei of all normal cells. It is thought that INI-1 acts an an adaptor protein, recruiting the SWI/SNF complex to the site of transcription where it can regulate transcription (La Porte et al. 2016). The protein INI-1 is a potent tumor suppressor. Inactivation of the gene encoding INI-1 has been implicated in pathogenesis of a range of malignant neoplasms that have rhabdoid cytomorphology, defined as large cells with specific nuclear and cytoplasmic features (Bishop et al. 2014). In addition to INI-1, over 20 members of the SWI/SNF complex have been found to be altered in human tumors (Biegel et al. 2014). INI-1 incorporates into HIV-1 virons and plays important roles in HIV-1 transcription and particle production (La Porte et al. 2016). |
|
- Target Species
- Human
- Western Blotting
- Mouse anti INI-1 detects a band of approximately 44 kDa in Jurkat cell lysates
- Species Cross-Reactivity
-
Target Species Cross Reactivity Mouse Rat - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - Liquid
- Preparation
- Mouse monoclonal antibody affinity purified on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide
- Immunogen
- E. coli--derived recombinant protein of amino acids 81-181 of human INI-1
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunised BALB/c mice were fused with cells of the mouse SP2/0 myeloma cell line
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
- Acknowledgements
- PrecisionAb is a trademark of Bio-Rad Laboratories
Store undiluted at -20°C, avoiding repeated freeze thaw cycles
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Western Blotting |  |
1/1000 |
The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Goat anti Mouse IgG (H/L):HRP | STAR207P | WB | 2 ml |
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| List Price | Your Price | ||||||
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| Description | Goat anti Mouse IgG (H/L):HRP | ||||||
- UniProt
- Q12824
- Entrez Gene
- SMARCB1
- GO Terms
- GO:0000228 nuclear chromosome
- GO:0002039 p53 binding
- GO:0005654 nucleoplasm
- GO:0005730 nucleolus
- GO:0006338 chromatin remodeling
- GO:0006350 transcription
- GO:0007049 cell cycle
- GO:0007399 nervous system development
- GO:0010552 positive regulation of gene-specific transcription from RNA polymerase II promoter
- View More GO Terms
- GO:0015074 DNA integration
- GO:0016514 SWI/SNF complex
- GO:0044419 interspecies interaction between organisms
- GO:0045090 retroviral genome replication
- GO:0051091 positive regulation of transcription factor activity
- GO:0071564 npBAF complex
- GO:0071565 nBAF complex
VMA00734
100004126
100007437
If you cannot find the batch/lot you are looking for please contact our technical support team for assistance.
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