ILF2 antibody | OTI6F1

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Mouse anti ILF2

ILF2 antibody recognizes interleukin enhancer-binding factor 2, also known as nuclear factor of activated T-cells. This antibody has been extensively validated for western blotting using whole cell lysates.

Product Type
PrecisionAb™ Monoclonal
Clone
OTI6F1
Isotype
IgG1
Format
Purified
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
VMA00182 WB 100 µl
Mouse anti Human ILF2 antibody recognizes ILF2, also known as interleukin enhancer binding factor 2 and nuclear factor of activated T-cells, 45-kDa.

The protein encoded by the ILF2 gene is the 45 kDa component of nuclear factor of activated T-cells (NFAT), a heterodimer of 45 kDa and 90 kDa proteins. NFAT is a transcription factor required for T-cell expression of the interleukin 2 gene. It also binds RNA and is an essential component for encapsidation and protein priming of hepatitis B viral polymerase. The complex has been shown to repair DNA breaks by nonhomologous end joining and can also negatively regulate the microRNA processing pathway. Alternative splicing results in multiple transcript variants. Related pseudogenes have been found on chromosomes 3 and 14 (provided by RefSeq, Jun 2012).

Mouse anti Human ILF2 antibody detects a band of 45 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species
Human
Western Blotting
Anti ILF2 detects a band of approximately 45 kDa in MOLT-4 cell lysates.
Product Form
Purified IgG - liquid
Preparation
Mouse monoclonal antibody purified by affinity chromatography from ascites.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
50% Glycerol
Immunogen
Full length protein expressed in 293T cell transfected with human ILF2 expression vector
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Shelf Life
As supplied, 12 months from date of despatch.

More Information

UniProt
Q12905 Related reagents
Entrez Gene
ILF2 Related reagents
GO Terms
GO:0005515 protein binding
GO:0003677 DNA binding
GO:0003702 RNA polymerase II transcription factor activity
GO:0003725 double-stranded RNA binding
GO:0030529 ribonucleoprotein complex
GO:0005524 ATP binding
GO:0005737 cytoplasm
GO:0005730 nucleolus
GO:0006350 transcription
GO:0006955 immune response
GO:0016740 transferase activity
GO:0045893 positive regulation of transcription, DNA-dependent
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Regulatory
For research purposes only

Applications of ILF2 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Goat anti Mouse IgG (H/L):HRP STAR207P 2 ml WB

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