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IL-6 antibody | J06-1E12

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Anti Human Interleukin-6 Antibody, clone J06-1E12 thumbnail image 1
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Sandwich ELISA analysis of human interleukin-6 using Mouse anti Human interleukin-6 (MCA6333GA) as a capture reagent and biotinylated Mouse anti Human interleukin-6 (MCA6334B) as a detection reagent with purified recombinant interleukin-6 as the antigen. Detection was performed using HRP conjugated streptavidin and substrate.

Mouse anti Human Interleukin-6:Biotin

Product Type
Monoclonal Antibody
Clone
J06-1E12
Isotype
IgG1
Specificity
IL-6

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Product Code Applications Pack Size List Price Your Price Qty
MCA6334B
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Mouse anti Human interleukin-6 antibody, clone J06-1E12 recognizes human interleukin 6, also known as B-cell stimulatory factor 2, CTL differentiation factor, Hybridoma growth factor or Interferon beta-2. Interleukin-6 is a 183 amino acid ~24 kDa cytokine with a wide range of biological activities. Interleukin-6 is a potent inducer of the acute phase response, acts as a lymphoid cell growth factor acting on B cells, T cells and hybridoma cells.

Variations in the interleukin-6 gene modulates susceptibility to onset of juvenile systemic rheumatoid arthritis (RASJ) an inflammatory disorder with early onset and featuring lymphadenopathy, hepatosplenomegaly, serositis, myalgia and arthritis (Fishman et al. 1998).

The biotinylated Mouse anti Human interleukin-6 antibody, clone J06-1E12 (MCA6334B) can be used as a detection antibody in a sandwich ELISA with the purified Mouse anti Human interleukin-6 antibody, clone C01-1E8 (MCA6333GA) as the capture antibody.

Target Species
Human
Product Form
Purified IgG conjugated to Biotin - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
Immunogen
E. coli derived recombinant human full length interleukin-6 (Val30-Met212)
Approx. Protein Concentrations
IgG concentration 0.1 mg/ml
Fusion Partners
Cell fusion between immunized BALB/c mouse spleen cells and mouse myeloma SP2/0
ELISA Matched Pair(s)
ELISA Matched Pair-Capture Antibody: MCA6333GA
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1.0 ug/ml
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

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UniProt
P05231
Entrez Gene
IL6
GO Terms
GO:0001781 neutrophil apoptosis
GO:0002384 hepatic immune response
GO:0002446 neutrophil mediated immunity
GO:0002548 monocyte chemotaxis
GO:0002675 positive regulation of acute inflammatory response
GO:0002690 positive regulation of leukocyte chemotaxis
GO:0006953 acute-phase response
GO:0005125 cytokine activity
GO:0005138 interleukin-6 receptor binding
GO:0005615 extracellular space
GO:0005896 interleukin-6 receptor complex
GO:0006959 humoral immune response
GO:0008083 growth factor activity
GO:0050830 defense response to Gram-positive bacterium
GO:0050829 defense response to Gram-negative bacterium
GO:0008285 negative regulation of cell proliferation
GO:0010574 regulation of vascular endothelial growth factor production
GO:0010888 negative regulation of lipid storage
GO:0045727 positive regulation of translation
GO:0030168 platelet activation
GO:0031018 endocrine pancreas development
GO:0031175 neuron projection development
GO:0032494 response to peptidoglycan
GO:0032722 positive regulation of chemokine production
GO:0032755 positive regulation of interleukin-6 production
GO:0032966 negative regulation of collagen biosynthetic process
GO:0033138 positive regulation of peptidyl-serine phosphorylation
GO:0042102 positive regulation of T cell proliferation
GO:0042517 positive regulation of tyrosine phosphorylation of Stat3 protein
GO:0043066 negative regulation of apoptosis
GO:0043193 positive regulation of gene-specific transcription
GO:0043410 positive regulation of MAPKKK cascade
GO:0045079 negative regulation of chemokine biosynthetic process
GO:0045599 negative regulation of fat cell differentiation
GO:0045669 positive regulation of osteoblast differentiation
GO:0045765 regulation of angiogenesis
GO:0045768 positive regulation of anti-apoptosis
GO:0045944 positive regulation of transcription from RNA polymerase II promoter
GO:0048661 positive regulation of smooth muscle cell proliferation
GO:0050871 positive regulation of B cell activation
GO:0051024 positive regulation of immunoglobulin secretion
GO:0051091 positive regulation of transcription factor activity
GO:0051384 response to glucocorticoid stimulus
GO:0051607 defense response to virus
GO:0070091 glucagon secretion
GO:0070102 interleukin-6-mediated signaling pathway

MCA6334B

100005527

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