IgG1 antibody | AbD27686

Human anti Human IgG1 (Fc):HRP

Product Type
Monoclonal Antibody
Clone
AbD27686
Isotype
HuCAL Fab monovalent
Specificity
IgG1

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HCA285P
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Human Anti-Human IgG1 Fc Antibody clone AbD27686 is a recombinant monovalent Fab antibody, that recognizes human IgG1 Fc, with an affinity of 76 nM. It has been shown to have a greatly reduced affinity (89-fold) for IgG from cynomolgus macaque compared to human IgG1. It also has strongly reduced binding to rhesus macaque IgG. It does not recognize other human immunoglobulin isotypes (IgG2, IgG3, IgG4, IgA, IgE, IgM) or mouse IgG2a.

This antibody has been generated by Bio-Rad using the HuCAL® antibody library and CysDisplay® technology. It is sold for research use, with implicit permission for use for the detection of therapeutic antibodies from samples obtained from experimental animals. When tested by Bio-Rad in an antigen capture pharmacokinetic assay format, this antibody performs similarly to Mouse Anti-Human IgG (CH2 Domain) Antibody clone R10Z8E9 (MCA5748G) in 10% cynomolgus macaque serum. It recognizes a different epitope on human IgG1 Fc than clone R10Z8E9.

View a summary of anti-human IgG Fc specific antibodies for the detection of therapeutic antibodies.

Target Species
Human
Product Form
A monovalent human recombinant Fab (lambda light chain) selected from the HuCAL PLATINUM phage display library. Expressed in E. coli and purified using purified using NiNTA affinity chromatography. The antibody is tagged with a DYKDDDDK tag and a HIS-tag (HHHHHH) at the C-terminus of the antibody heavy chain. Conjugated to horseradish peroxidase (HRP) - liquid.
Buffer Solution
Phosphate buffered saline.
Preservative Stabilisers
0.01% Thiomersal.
Immunogen
Recombinant human IgG1 kappa
Affinity
The intrinsic affinity of the monovalent form of this antibody is KD=76 nM as measured by real time, label free molecular interaction analysis on immobilized hIgG1 kappa.
Approx. Protein Concentrations
Antibody concentration 0.1 mg/ml.
Regulatory
For research purposes only.
Guarantee
12 months from date of despatch.
Acknowledgements
This product and/or its use is covered by claims of U.S. patents, and/or pending U.S. and non-U.S. patent applications owned by or under license to Bio-Rad Laboratories, Inc. See bio-rad.com/en-us/trademarks for details.
His-tag is a registered trademark of EMD Biosciences.

This product is shipped frozen.
When ready to use, thaw and aliquot the sample as needed. Store aliquots at -70°C, if available, otherwise store at -20°C. It is not recommended to keep aliquots at 4°C for more than one week.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1/100 1/1000
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Technical Advice
Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual.

Description Product Code Applications Pack Size List Price Your Price Quantity
Hispec Assay Diluent BUF049A E IY 50 ml
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Description Hispec Assay Diluent

Licensed Use
For in vitro research purposes only, unless otherwise specified in writing by Bio-Rad.
View more products with IGG specificity

Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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