IGF2BP-2 antibody | OTI3C8

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Mouse anti IGF2BP-2

Mouse anti human IGF2BP-2 antibody recognizes insulin-like growth factor 2 mRNA-binding protein 2, also known as IGF2 binding protein 2 or VICKZ family member 2. This antibody has been extensively validated for western blotting using whole cell lysates.

IGF2BP-2 Antibody with Control Lysate

Mouse anti human IGF2BP-2 antibody recognizes insulin-like growth factor 2 mRNA-binding protein 2, also known as IGF2 binding protein 2 or VICKZ family member 2. This antibody has been extensively validated for western blotting using whole cell lysates.

Product Type
PrecisionAb™ Monoclonal
Clone
OTI3C8
Isotype
IgG1
Format
Purified

Select your package type:

Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
VMA00401 WB 100 µl
VMA00401KT WB 2 Westerns
With Lysate 400µg
Mouse anti Human IGF2BP-2 antibody recognizes insulin-like growth factor 2 mRNA-binding protein 2 (IGFBP-2), also known as IGF2 binding protein 2 and VICKZ family member 2.

IGF2BP-2 is a member of the IGF-II mRNA-binding protein (IMP) family. It functions by binding to the 5' UTR of the insulin-like growth factor 2 (IGF2) mRNA and regulating IGF2 translation. Alternate transcriptional splice variants, encoding different isoforms, have been characterized (provided by RefSeq, Jul 2008).

Mouse anti IGF2BP-2 antibody detects bands of 62 and 66 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species
Human
Western Blotting
Anti IGF2BP-2 detects a bands of 62 and 66 kDa in HepG2 cell lysates
Product Form
Purified IgG - liquid
Product Form
Purified IgG - liquid
Preparation
Mouse monoclonal antibody purified by affinity chromatography from ascites
Preparation
20μl Mouse monoclonal antibody purified by affinity chromatography from ascites
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
50% Glycerol
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
50% Glycerol
Immunogen
Full length recombinant human IGF2BP-2 (NP_006539) produced in HEK293T cells
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.

Positive Control Lysate

Lysate Composition
400μg HepG2 lysate lyophilized in RIPA buffer
Lysate Reconstitution
- If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
- If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Storage
Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
Shelf Life
As supplied, 12 months from date of despatch.
Shelf Life
As supplied, 12 months from date of despatch

More Information

UniProt
Q9Y6M1 Related reagents
Entrez Gene
IGF2BP2 Related reagents
GO Terms
GO:0000166 nucleotide binding
GO:0005515 protein binding
GO:0003730 mRNA 3'-UTR binding
GO:0005829 cytosol
GO:0005634 nucleus
GO:0009653 anatomical structure morphogenesis
GO:0017148 negative regulation of translation
GO:0042035 regulation of cytokine biosynthetic process
GO:0044430 cytoskeletal part
GO:0045182 translation regulator activity
GO:0048027 mRNA 5'-UTR binding
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories
Regulatory
For research purposes only

Applications of IGF2BP-2 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000
Western Blotting 1/1000

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Goat anti Mouse IgG (H/L):HRP STAR207P 2 ml WB
Goat anti Mouse IgG (H/L):HRP STAR207P 2 ml WB

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