IGF2BP-2 antibody | OTI3C8
Mouse anti IGF2BP-2
Mouse anti human IGF2BP-2 antibody recognizes insulin-like growth factor 2 mRNA-binding protein 2, also known as IGF2 binding protein 2 or VICKZ family member 2. This antibody has been extensively validated for western blotting using whole cell lysates.
IGF2BP-2 Antibody with Control Lysate
Mouse anti human IGF2BP-2 antibody recognizes insulin-like growth factor 2 mRNA-binding protein 2, also known as IGF2 binding protein 2 or VICKZ family member 2. This antibody has been extensively validated for western blotting using whole cell lysates.
- Product Type
- PrecisionAb™ Monoclonal
- Clone
- OTI3C8
- Isotype
- IgG1
- Format
- Purified
| Product Code | Applications | Datasheet | MSDS | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|---|
| VMA00401 | WB | 100 µl | ||||
| VMA00401KT | WB | 2 Westerns | ||||
| With Lysate | 400µg |
Mouse anti Human IGF2BP-2 antibody recognizes insulin-like growth factor 2 mRNA-binding protein 2 (IGFBP-2), also known as IGF2 binding protein 2 and VICKZ family member 2.
IGF2BP-2 is a member of the IGF-II mRNA-binding protein (IMP) family. It functions by binding to the 5' UTR of the insulin-like growth factor 2 (IGF2) mRNA and regulating IGF2 translation. Alternate transcriptional splice variants, encoding different isoforms, have been characterized (provided by RefSeq, Jul 2008).
Mouse anti IGF2BP-2 antibody detects bands of 62 and 66 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
IGF2BP-2 is a member of the IGF-II mRNA-binding protein (IMP) family. It functions by binding to the 5' UTR of the insulin-like growth factor 2 (IGF2) mRNA and regulating IGF2 translation. Alternate transcriptional splice variants, encoding different isoforms, have been characterized (provided by RefSeq, Jul 2008).
Mouse anti IGF2BP-2 antibody detects bands of 62 and 66 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
Product Details
- Target Species
- Human
- Western Blotting
- Anti IGF2BP-2 detects a bands of 62 and 66 kDa in HepG2 cell lysates
- Product Form
- Purified IgG - liquid
- Product Form
- Purified IgG - liquid
- Preparation
- Mouse monoclonal antibody purified by affinity chromatography from ascites
- Preparation
- 20μl Mouse monoclonal antibody purified by affinity chromatography from ascites
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
50% Glycerol - Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
50% Glycerol - Immunogen
- Full length recombinant human IGF2BP-2 (NP_006539) produced in HEK293T cells
- Instructions For Use
- Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
Positive Control Lysate
- Lysate Composition
- 400μg HepG2 lysate lyophilized in RIPA buffer
- Lysate Reconstitution
- - If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
- If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.
Storage Information
- Storage
- Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
- Storage
- Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage. - Shelf Life
- As supplied, 12 months from date of despatch.
- Shelf Life
- As supplied, 12 months from date of despatch
More Information
- UniProt
- Q9Y6M1 Related reagents
- Entrez Gene
- IGF2BP2 Related reagents
- GO Terms
- GO:0000166 nucleotide binding
- GO:0005515 protein binding
- GO:0003730 mRNA 3'-UTR binding
- GO:0005829 cytosol
- GO:0005634 nucleus
- GO:0009653 anatomical structure morphogenesis
- GO:0017148 negative regulation of translation
- GO:0042035 regulation of cytokine biosynthetic process
- GO:0044430 cytoskeletal part
- GO:0045182 translation regulator activity
- GO:0048027 mRNA 5'-UTR binding
- Acknowledgements
- PrecisionAb™ is a trademark of Bio-Rad Laboratories.
- Acknowledgements
- PrecisionAb™ is a trademark of Bio-Rad Laboratories
- Regulatory
- For research purposes only
Applications of IGF2BP-2 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Western Blotting | 1/1000 | ||
| Western Blotting | 1/1000 |
PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
Copyright © 2019 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Goat anti Mouse IgG (H/L):HRP | STAR207P | 2 ml | WB | ||
| Goat anti Mouse IgG (H/L):HRP | STAR207P | 2 ml | WB |
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