IgE antibody | 1A2
Horseradish peroxidase conjugated Mouse anti Human IgE antibody, clone 1A2 (MCA2116P) used to detect bound human IgE on immunoblots.
Image caption:
Comparison of surface fractions, cytoplasmic fractions, and commercial allergen extracts.
Nitrocellulose dot blots probed with sera pooled from 500 individuals and HRP-conjugated anti-IgE.
From: Vigh-Conrad KA, Conrad DF, Preuss D.
A protein allergen microarray detects specific IgE to pollen surface, cytoplasmic, and commercial allergen extracts.
PLoS One. 2010 Apr 16;5(4):e10174.
doi: 10.1371/journal.pone.0010174.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Mouse anti Human IgE:HRP
- Product Type
- Monoclonal Antibody
- Clone
- 1A2
- Isotype
- IgG1
- Specificity
- IgE
Antibody Quality and Performance Guarantee
| Mouse anti Human IgE antibody, clone 1A2 recognizes human IgE and no cross-reactivity to other human immunoglobulin classes or subclasses has been detected. |
- Target Species
- Human
- Product Form
- Purified IgG conjugated to horseradish peroxidase (HRP) - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.01% Thiomersal
- Immunogen
- Chimaeric human IgE.
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |  |
1/500 | 1/1000 |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| AbGUARD® HRP Stabilizer Plus | BUF052A | C E P WB | 100 ml | Log in | |||
| List Price | Your Price | ||||||
| Log in | |||||||
| Description | AbGUARD® HRP Stabilizer Plus | ||||||
| AbGUARD® HRP Stabilizer Plus | BUF052B | C E P WB | 500 ml | Log in | |||
| List Price | Your Price | ||||||
| Log in | |||||||
| Description | AbGUARD® HRP Stabilizer Plus | ||||||
| AbGUARD® HRP Stabilizer Plus | BUF052C | C E P WB | 1000 ml | Log in | |||
| List Price | Your Price | ||||||
| Log in | |||||||
| Description | AbGUARD® HRP Stabilizer Plus | ||||||
| TMB Signal+ | BUF054A | E | 100 ml | Log in | |||
| List Price | Your Price | ||||||
| Log in | |||||||
| Description | TMB Signal+ | ||||||
| TMB Core | BUF056A | E | 100 ml | Log in | |||
| List Price | Your Price | ||||||
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| Description | TMB Core | ||||||
| TMB Core+ | BUF062A | E | 100 ml | Log in | |||
| List Price | Your Price | ||||||
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| Description | TMB Core+ | ||||||
References for IgE antibody
-
Vigh-Conrad, K.A. et al. (2010) A protein allergen microarray detects specific IgE to pollen surface, cytoplasmic, and commercial allergen extracts.
PLoS One. 5(4): e10174. -
Sogawa, K. et al. (2018) Search for a Novel Allergen in Hen's Egg Allergy Using an IgE Immunoblotting Assay.
Int Arch Allergy Immunol. 176 (3-4): 189-97.
- RRID
- AB_324465
- UniProt
- P01854
- Entrez Gene
- IGHE
- GO Terms
- GO:0003823 antigen binding
- GO:0006955 immune response
MCA2116P
154268
163641
210114R
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