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IgE antibody | 1A2

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Anti Human IgE Antibody, clone 1A2 gallery image 1
Sandwich ELISA analysis of human IgE using Mouse anti Human IgE (MCA2115) as a capture reagent and horseradish peroxidase conjugated Mouse anti Human IgE (MCA2116P) as a detection reagent with purified human IgE as antigen. Microtitre plate is read at O.D. 450 nm on the Bio-Rad iMark Microplate Absorbance Reader (Bio-Rad Catalog 168-1135). A serum sample at 1/10 (blue) and 1/5 (green) and a plasma sample at 1/10 (red) and1/5 (orange) is shown

Mouse anti Human IgE:HRP

Product Type
Monoclonal Antibody
Clone
1A2
Isotype
IgG1
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
MCA2116P E 0.5 mg
Mouse anti Human IgE antibody, clone 1A2 recognizes human IgE and no cross-reactivity to other human immunoglobulin classes or subclasses has been detected.

Product Details

Target Species
Human
Product Form
Purified IgG conjugated to Horseradish Peroxidase (HRP) - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.01%Thiomersal
Immunogen
Chimaeric human IgE.
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.

More Information

UniProt
P01854 Related reagents
Entrez Gene
IGHE Related reagents
GO Terms
GO:0003823 antigen binding
GO:0006955 immune response
Regulatory
For research purposes only

Applications of IgE antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1/500 1/1000
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Copyright © 2018 Bio-Rad Antibodies (formerly AbD Serotec)

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
AbGUARD® HRP Stabilizer Plus BUF052A 100 ml C E P WB
AbGUARD® HRP Stabilizer Plus BUF052B 500 ml C E P WB
AbGUARD® HRP Stabilizer Plus BUF052C 1000 ml C E P WB
TMB Signal+ BUF054A 100 ml E
TMB Core BUF056A 100 ml E
TMB Core+ BUF062A 100 ml E

Product Specific References

References for IgE antibody

  1. Vigh-Conrad, K.A. et al. (2010) A protein allergen microarray detects specific IgE to pollen surface, cytoplasmic, and commercial allergen extracts.
    PLoS One. 5(4): e10174.
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