IgE antibody | 1A2

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Anti Human IgE Antibody, clone 1A2 gallery image 1 — Sandwich ELISA analysis of human IgE using Mouse anti Human IgE antibody as a capture reagent and horseradish peroxidase conjugated Mouse anti Human IgE (**MCA2116P**) as a detection reagent with purified human IgE as antigen. Microtitre plate is read at O.D. 450 nm on the Bio-Rad iMark Microplate Absorbance Reader (Bio-Rad Catalog 168-1135). A serum sample at 1/10 (blue) and 1/5 (green) and a plasma sample at 1/10 (red) and1/5 (orange) is shown

Mouse anti Human IgE:HRP

Product Type: Monoclonal Antibody
Clone: 1A2
Isotype: IgG1

Product Code	Applications	Datasheet	MSDS	Pack Size	List Price	Quantity
MCA2116P	E			0.5 mg

Mouse anti Human IgE antibody, clone 1A2 recognizes human IgE and no cross-reactivity to other human immunoglobulin classes or subclasses has been detected.

Product Details

Target Species

Human

Product Form

Purified IgG conjugated to Horseradish Peroxidase (HRP) - liquid

Preparation

Purified IgG prepared by affinity chromatography on Protein A

Buffer Solution

Phosphate buffered saline

Preservative Stabilisers

0.01%

Thiomersal

Immunogen

Chimaeric human IgE.

Approx. Protein Concentrations

IgG concentration 1.0 mg/ml

Storage Information

Storage: Store at +4^oC or at -20^oC if preferred.

This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 18 months from date of despatch.

More Information

UniProt: P01854 Related reagents
Entrez Gene: IGHE Related reagents
GO Terms: GO:0003823 antigen binding; GO:0006955 immune response
Regulatory: For research purposes only

Applications of IgE antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
ELISA		1/500	1/1000

Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.

Useful Reagents Available

Description	Product Code	Pack Size	Applications
AbGUARD® HRP Stabilizer Plus	BUF052A	100 ml	C E P WB
AbGUARD® HRP Stabilizer Plus	BUF052B	500 ml	C E P WB
AbGUARD® HRP Stabilizer Plus	BUF052C	1000 ml	C E P WB
TMB Signal+	BUF054A	100 ml	E
TMB Core	BUF056A	100 ml	E
TMB Core+	BUF062A	100 ml	E

Product Specific References

References for IgE antibody

Vigh-Conrad, K.A. et al. (2010) A protein allergen microarray detects specific IgE to pollen surface, cytoplasmic, and commercial allergen extracts.
PLoS One. 5(4): e10174.

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