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IFN Gamma antibody | 18F11G6

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Sandwich ELISA analysis of human IFN gamma using Mouse anti Human IFN gamma (MCA6238GA) as a capture reagent and biotinylated Mouse anti Human IFN gamma (MCA6239B) as a detection reagent with purified recombinant IFN gamma as the antigen. Detection was performed using HRP conjugated streptavidin and substrate.

Mouse anti Human IFN Gamma:Biotin

Product Type
Monoclonal Antibody
Clone
18F11G6
Isotype
IgG2a
Specificity
IFN Gamma

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Product Code Applications Pack Size List Price Your Price Qty
MCA6239B
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Mouse anti Human IFN gamma antibody, clone 18F11G6, recognizes interferon gamma (IFN gamma) also known as immune interferon, a type II interferon. It is a cytokine which is mainly expressed by CD4+ Th1 T lymphocytes and natural killer cells. IFN gamma plays many roles within immunity, including the activation of macrophages, T helper lymphocytes, stimulating antigen presentation, inflammation and leukocyte trafficking (Pereiro et al. 2019).

After infection antigen presenting cells secrete IL-12, IL-18 and IL-1 beta which can promote IFN gamma synthesis (Schoenborn & Wilson 2007). IFN gamma can then elicit responses through interferon gamma receptor 1 and interferon gamma receptor 2. Activation of these receptors can then trigger immune responses through the JAK/STAT signaling pathway (Pereiro et al. 2019).

Under physiological conditions, IFN gamma is constitutively expressed through a tightly controlled mechanism. However, it has been shown to have some protumorigenic roles, by upregulating checkpoint inhibitor programmed cell-death ligand 1. Conversely though, there have been some positive associations between IFN gamma and patient survival in several cancer types. As such, the exact roles that IFN gamma plays in cancer may largely be dependent on tumor specific context and microenvironmental cues (Castro et al. 2018).

The biotinylated Mouse anti Human IFN gamma antibody, clone 18F11G6 (MCA6239B) can be used as a detection antibody in a sandwich ELISA with the purified Mouse anti Human IFN gamma antibody, clone 8C4H9 (MCA6238GA) as the capture antibody.

Target Species
Human
Product Form
Purified IgG conjugated to Biotin - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
Immunogen
E. coli derived recombinant human full length IFN gamma (Gln24-Gln166)
Approx. Protein Concentrations
IgG concentration 0.1 mg/ml
Fusion Partners
Cell fusion between immunized BALB/c mouse spleen cells and mouse myeloma SP2/0
ELISA Matched Pair(s)
ELISA Matched Pair-Capture Antibody: MCA6238GA
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1.0 ug/ml
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

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UniProt
P01579
Entrez Gene
IFNG
GO Terms
GO:0000060 protein import into nucleus, translocation
GO:0002053 positive regulation of mesenchymal cell proliferation
GO:0003340 negative regulation of mesenchymal to epithelial transition involved in metanephros morphogenesis
GO:0009615 response to virus
GO:0005125 cytokine activity
GO:0005133 interferon-gamma receptor binding
GO:0005615 extracellular space
GO:0006955 immune response
GO:0007050 cell cycle arrest
GO:0050796 regulation of insulin secretion
GO:0010553 negative regulation of gene-specific transcription from RNA polymerase II promoter
GO:0032735 positive regulation of interleukin-12 production
GO:0032747 positive regulation of interleukin-23 production
GO:0033141 positive regulation of peptidyl-serine phosphorylation of STAT protein
GO:0034393 positive regulation of smooth muscle cell apoptosis
GO:0042508 tyrosine phosphorylation of Stat1 protein
GO:0042511 positive regulation of tyrosine phosphorylation of Stat1 protein
GO:0045429 positive regulation of nitric oxide biosynthetic process
GO:0045672 positive regulation of osteoclast differentiation
GO:0048662 negative regulation of smooth muscle cell proliferation
GO:0051044 positive regulation of membrane protein ectodomain proteolysis
GO:0051712 positive regulation of killing of cells of other organism
GO:0060333 interferon-gamma-mediated signaling pathway
GO:0060334 regulation of interferon-gamma-mediated signaling pathway
GO:0060550 positive regulation of fructose 1,6-bisphosphate 1-phosphatase activity
GO:0060552 positive regulation of fructose 1,6-bisphosphate metabolic process
GO:0060557 positive regulation of vitamin D biosynthetic process
GO:0060559 positive regulation of calcidiol 1-monooxygenase activity
GO:0072308 negative regulation of metanephric nephron tubule epithelial cell differentiation

MCA6239B

100005420

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