hnRNP F antibody | OTI5F5

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Anti hnRNP F Antibody, clone OTI5F5 (PrecisionAb Monoclonal Antibody) thumbnail image 1

Anti hnRNP F Antibody, clone OTI5F5 (PrecisionAb Monoclonal Antibody) thumbnail image 2

Anti hnRNP F Antibody, clone OTI5F5 (PrecisionAb Monoclonal Antibody) thumbnail image 3

Anti hnRNP F Antibody, clone OTI5F5 (PrecisionAb Monoclonal Antibody) gallery image 1 — Western blot analysis of hnRNP F knockout HEK293 and wild type HEK293 (WT) whole cell lysates probed with (A) Mouse anti hnRNP F antibody (**VMA00113**) followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000 STAR207P) and (B) hFAB rhodamine anti-tubulin primary antibody (12004166) and visualized on the ChemiDoc MP with a (A) 12 second and (B) 30 second exposure.

Anti hnRNP F Antibody, clone OTI5F5 (PrecisionAb Monoclonal Antibody) gallery image 2 — Normalized signal intensity of hnRNP F from CRISPR knockout and wild type lysates. Signal intensity was normalized to the tubulin signal.

Anti hnRNP F Antibody, clone OTI5F5 (PrecisionAb Monoclonal Antibody) gallery image 3 — Western blot analysis of whole cell lysates probed with Mouse anti Human hnRNP F antibody, clone OTI5F5 (**VMA00113**) followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000, STAR207P) and visualized on the ChemiDoc MP with 47 second exposure. Arrow points to hnRNP F (molecular weight 46 kDa).

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Mouse anti hnRNP F

Product Type: PrecisionAb Monoclonal
Clone: OTI5F5
Isotype: IgG2a
Format: Purified
Specificity: hnRNP F

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VMA00113 Datasheet Datasheet SDS SDS	WB	100 µl		Log in	Get Quote
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Mouse anti Human hnRNP F antibody, clone OTI5F5 recognizes heterogeneous nuclear ribonucleoprotein F (hnRNP F), also known as nucleolin-like protein mcs94-1.

HnRNP F belongs to the subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). The hnRNPs are RNA binding proteins that complex with heterogeneous nuclear RNA (hnRNA). These proteins are associated with pre-mRNAs in the nucleus and regulate alternative splicing, polyadenylation, and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus, some seem to shuttle between the nucleus and the cytoplasm. The hnRNP proteins have distinct nucleic acid binding properties. Heterogeneous nuclear ribonucleoprotein F has three repeats of quasi-RRM domains that bind to RNAs which have guanosine-rich sequences. This protein is very similar to the family member hnRPH. Multiple alternatively spliced variants, encoding the same protein, have been identified (provided by RefSeq, Jul 2008).

Mouse anti Human hnRNP F antibody detects a band of 46 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Target Species: Human
Western Blotting: Anti hnRNP F detects a band of approximately 46 kDa in Jurkat cell lysates.
Species Cross-Reactivity: Target Species Cross Reactivity

Mouse

Rat; N.B. Antibody reactivity and working conditions may vary between species.
Product Form: Purified IgG - liquid
Preparation: Mouse monoclonal antibody purified by affinity chromatography from ascites.
Buffer Solution: Phosphate buffered saline.
Preservative Stabilisers: 0.09% Sodium Azide (NaN₃)
1% Bovine Serum Albumin
50% Glycerol
Immunogen: Full length recombinant human hnRNP F (NP_004957) produced in HEK293T cells
Approx. Protein Concentrations: IgG concentration 0.52 mg/ml.
Regulatory: For research purposes only.
Guarantee: 12 months from date of despatch.
Acknowledgements: PrecisionAb is a trademark of Bio-Rad Laboratories.

Target Species	Cross Reactivity
Mouse
Rat

Store undiluted at -20°C, avoiding repeated freeze thaw cycles.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Western Blotting		1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Description	Product Code	Applications	Pack Size	Your Price
Goat anti Mouse IgG (H/L):HRP	STAR207P	WB	2 ml	Log in	Get Quote
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Description	Goat anti Mouse IgG (H/L):HRP

UniProt: P52597
Entrez Gene: HNRNPF
GO Terms: GO:0000166 nucleotide binding; GO:0005515 protein binding; GO:0000398 nuclear mRNA splicing, via spliceosome; GO:0003727 single-stranded RNA binding; GO:0005737 cytoplasm; GO:0005654 nucleoplasm; GO:0005681 spliceosomal complex; GO:0030530 heterogeneous nuclear ribonucleoprotein complex; GO:0043484 regulation of RNA splicing; View More GO Terms