hnRNP F antibody | OTI5F5
Mouse anti Human hnRNP F antibody, clone OTI5F5 recognizes heterogeneous nuclear ribonucleoprotein F (hnRNP F), also known as nucleolin-like protein mcs94-1.
HnRNP F belongs to the subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). The hnRNPs are RNA binding proteins that complex with heterogeneous nuclear RNA (hnRNA). These proteins are associated with pre-mRNAs in the nucleus and regulate alternative splicing, polyadenylation, and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus, some seem to shuttle between the nucleus and the cytoplasm. The hnRNP proteins have distinct nucleic acid binding properties. Heterogeneous nuclear ribonucleoprotein F has three repeats of quasi-RRM domains that bind to RNAs which have guanosine-rich sequences. This protein is very similar to the family member hnRPH. Multiple alternatively spliced variants, encoding the same protein, have been identified (provided by RefSeq, Jul 2008).
Mouse anti Human hnRNP F antibody detects a band of 46 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
HnRNP F belongs to the subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). The hnRNPs are RNA binding proteins that complex with heterogeneous nuclear RNA (hnRNA). These proteins are associated with pre-mRNAs in the nucleus and regulate alternative splicing, polyadenylation, and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus, some seem to shuttle between the nucleus and the cytoplasm. The hnRNP proteins have distinct nucleic acid binding properties. Heterogeneous nuclear ribonucleoprotein F has three repeats of quasi-RRM domains that bind to RNAs which have guanosine-rich sequences. This protein is very similar to the family member hnRPH. Multiple alternatively spliced variants, encoding the same protein, have been identified (provided by RefSeq, Jul 2008).
Mouse anti Human hnRNP F antibody detects a band of 46 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
Product Details
- Target Species
- Human
- Western Blotting
- Anti hnRNP F detects a band of approximately 46 kDa in Jurkat cell lysates.
- Species Cross-Reactivity
-
Target Species Cross Reactivity Mouse Rat - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Preparation
- Mouse monoclonal antibody purified by affinity chromatography from ascites.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
50% Glycerol - Immunogen
- Full length recombinant human hnRNP F (NP_004957) produced in HEK293T cells
- Approx. Protein Concentrations
- IgG concentration 0.52 mg/ml
Storage Information
- Storage
- Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
- Guarantee
- 12 months from date of despatch
More Information
- UniProt
- P52597
- Entrez Gene
- HNRNPF
- GO Terms
- GO:0000166 nucleotide binding
- GO:0005515 protein binding
- GO:0000398 nuclear mRNA splicing, via spliceosome
- GO:0003727 single-stranded RNA binding
- GO:0005737 cytoplasm
- GO:0005654 nucleoplasm
- GO:0005681 spliceosomal complex
- GO:0030530 heterogeneous nuclear ribonucleoprotein complex
- GO:0043484 regulation of RNA splicing
- Acknowledgements
- PrecisionAb is a trademark of Bio-Rad Laboratories.
- Regulatory
- For research purposes only
Applications of hnRNP F antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Western Blotting | 1/1000 |
The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.
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