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hnRNP F antibody | OTI5F5

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Anti hnRNP F Antibody, clone OTI5F5 (PrecisionAb Monoclonal Antibody) thumbnail image 1 Anti hnRNP F Antibody, clone OTI5F5 (PrecisionAb Monoclonal Antibody) thumbnail image 2 Anti hnRNP F Antibody, clone OTI5F5 (PrecisionAb Monoclonal Antibody) thumbnail image 3
Anti hnRNP F Antibody, clone OTI5F5 (PrecisionAb Monoclonal Antibody) gallery image 1
Western blot analysis of hnRNP F knockout HEK293 and wild type HEK293 (WT) whole cell lysates probed with (A) Mouse anti hnRNP F antibody (VMA00113) followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000 STAR207P) and (B) hFAB rhodamine anti-tubulin primary antibody (12004166) and visualized on the ChemiDoc MP with a (A) 12 second and (B) 30 second exposure.
Anti hnRNP F Antibody, clone OTI5F5 (PrecisionAb Monoclonal Antibody) gallery image 2
Normalized signal intensity of hnRNP F from CRISPR knockout and wild type lysates. Signal intensity was normalized to the tubulin signal.
Anti hnRNP F Antibody, clone OTI5F5 (PrecisionAb Monoclonal Antibody) gallery image 3
Western blot analysis of whole cell lysates probed with Mouse anti Human hnRNP F antibody, clone OTI5F5 (VMA00113) followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000, STAR207P) and visualized on the ChemiDoc™ MP with 47 second exposure. Arrow points to hnRNP F (molecular weight 46 kDa).

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Mouse anti hnRNP F

Product Type
PrecisionAb Monoclonal
Clone
OTI5F5
Isotype
IgG2a
Format
Purified
Specificity
hnRNP F

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VMA00113
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Mouse anti Human hnRNP F antibody, clone OTI5F5 recognizes heterogeneous nuclear ribonucleoprotein F (hnRNP F), also known as nucleolin-like protein mcs94-1.

HnRNP F belongs to the subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). The hnRNPs are RNA binding proteins that complex with heterogeneous nuclear RNA (hnRNA). These proteins are associated with pre-mRNAs in the nucleus and regulate alternative splicing, polyadenylation, and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus, some seem to shuttle between the nucleus and the cytoplasm. The hnRNP proteins have distinct nucleic acid binding properties. Heterogeneous nuclear ribonucleoprotein F has three repeats of quasi-RRM domains that bind to RNAs which have guanosine-rich sequences. This protein is very similar to the family member hnRPH. Multiple alternatively spliced variants, encoding the same protein, have been identified (provided by RefSeq, Jul 2008).

Mouse anti Human hnRNP F antibody detects a band of 46 kDa. The antibody has been extensively validated for western blotting using whole cell lysates. 		PrecisionAb Antibodies

Target Species
Human
Western Blotting
Anti hnRNP F detects a band of approximately 46 kDa in Jurkat cell lysates.
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Mouse monoclonal antibody purified by affinity chromatography from ascites.
Buffer Solution
Phosphate buffered saline.
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
50% Glycerol
Immunogen
Full length recombinant human hnRNP F (NP_004957) produced in HEK293T cells
Approx. Protein Concentrations
IgG concentration 0.52 mg/ml.
Regulatory
For research purposes only.
Guarantee
12 months from date of despatch.
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories.

Store undiluted at -20°C, avoiding repeated freeze thaw cycles.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000
The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Description Product Code Applications Pack Size List Price Your Price Quantity
Goat anti Mouse IgG (H/L):HRP STAR207P WB 2 ml loader
List Price Your Price
loader
Description Goat anti Mouse IgG (H/L):HRP

UniProt
P52597
Entrez Gene
HNRNPF
GO Terms
GO:0000166 nucleotide binding
GO:0005515 protein binding
GO:0000398 nuclear mRNA splicing, via spliceosome
GO:0003727 single-stranded RNA binding
GO:0005737 cytoplasm
GO:0005654 nucleoplasm
GO:0005681 spliceosomal complex
GO:0030530 heterogeneous nuclear ribonucleoprotein complex
GO:0043484 regulation of RNA splicing
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