HLA ABC antibody | W6/32
Mouse anti Human HLA ABC:StarBright Violet 670
- Product Type
- Monoclonal Antibody
- Clone
- W6/32
- Isotype
- IgG2a
- Specificity
- HLA ABC
Mouse anti Human HLA ABC antibody, clone W6/32 recognizes an antigenic determinant shared among products of the HLA A, B and C loci. Clone W6/32 recognizes a conformational epitope, reacting with HLA class I alpha3 and alpha2 domains. The major histocompatibility complex (MHC) is a cluster of genes that are important in the immune response to infections. In humans, this complex is referred to as the human leukocyte antigen (HLA) region. There are 3 major MHC class I proteins encoded by the HLA which are HLA A, HLA B and HLA C. These proteins are found on the surface of almost all nucleated somatic cells. Mouse anti Human HLA ABC antibody, clone W6/32 is routinely tested in flow cytometry on human peripheral blood lymphocytes. |
- Target Species
- Human
- Species Cross-Reactivity
-
Target Species Cross Reactivity Macaque Bovine Cynomolgus monkey Baboon Rhesus Monkey Chimpanzee Gorilla Shrew Goat Dog Guinea Pig Rabbit Mouse Chicken Amphibia - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG conjugated to StarBright Violet 670 - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
0.1% Pluronic F68
0.1% PEG 3350
0.05% Tween 20 - Immunogen
- Purified human tonsil lymphocyte membranes.
- Fusion Partners
- Spleen cells from immunized BALB/c mice were fused with cells of the mouse NS1/1-Ag4.1 myeloma cell line.
- Max Ex/Em
-
Fluorophore Excitation Max (nm) Emission Max (nm) StarBright Violet 670 401 667 - Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
- Acknowledgements
- This product is covered by U.S. Patent No. 10,150,841 and related U.S. and foreign counterparts
This product should be stored undiluted.
Application Name | Verified | Min Dilution | Max Dilution |
---|---|---|---|
Flow Cytometry | Neat |
- Flow Cytometry
- Use 5μl of the suggested working dilution to label 106 cells in 100μl. Best practices suggest a 5 minutes centrifugation at 6,000g prior to sample application.
How to Use the Spectraviewer
Watch the Tool Tutorial Video ▸- Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
- Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
- Select the lasers and filters you wish to include
- Select combined or multi-laser view to visualize the spectra
Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
---|---|---|---|---|---|---|---|
Human Seroblock | BUF070A | F | 50 Test | Log in | |||
List Price | Your Price | ||||||
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Description | Human Seroblock | ||||||
Human Seroblock | BUF070B | F | 200 Test | Log in | |||
List Price | Your Price | ||||||
Log in | |||||||
Description | Human Seroblock |
Source Reference
-
Barnstable, C.J. et al. (1978) Production of monoclonal antibodies to group A erythrocytes, HLA and other human cell surface antigens-new tools for genetic analysis.
Cell. 14 (1): 9-20.
References for HLA ABC antibody
-
Brodsky, F.M. & Parham, P. (1982) Evolution of HLA antigenic determinants: species cross-reactions of monoclonal antibodies.
Immunogenetics. 15 (2): 151-66. -
Neefjes, J.J. et al. (1986) A biochemical characterization of feline MHC products: unusually high expression of class II antigens on peripheral blood lymphocytes.
Immunogenetics. 23 (5): 341-7. -
Stern, P.L. et al. (1987) Class I-like MHC molecules expressed by baboon placental syncytiotrophoblast.
J Immunol. 138 (4): 1088-91. -
Jacobsen, C.N. et al. (1993) Reactivities of 20 anti-human monoclonal antibodies with leucocytes from ten different animal species.
Vet Immunol Immunopathol. 39 (4): 461-6. -
Verbeek, M.M. et al. (1995) T lymphocyte adhesion to human brain pericytes is mediated via very late antigen-4/vascular cell adhesion molecule-1 interactions.
J Immunol. 154 (11): 5876-84. -
Raftery, M.J. et al. (2002) Hantavirus infection of dendritic cells.
J Virol. 76: 10724-33. -
Dressel, R. et al. (2003) Differential effect of acute and permanent heat shock protein 70 overexpression in tumor cells on lysability by cytotoxic T lymphocytes.
Cancer Res. 63 (23): 8212-20. -
Ishitani, A. et al. (2003) Protein expression and peptide binding suggest unique and interacting functional roles for HLA-E, F, and G in maternal-placental immune recognition.
J Immunol. 171 (3): 1376-84.
View The Latest Product References
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Smith, A.C. et al. (2005) Interaction of the Salmonella-containing vacuole with the endocytic recycling system.
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Zuo, J. et al. (2008) The DNase of gammaherpesviruses impairs recognition by virus-specific CD8+ T cells through an additional host shutoff function.
J Virol. 82: 2385-93. -
Shi, J. et al. (2008) Bortezomib down-regulates the cell-surface expression of HLA class I and enhances natural killer cell-mediated lysis of myeloma.
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Watling, D. et al. (2008) Multiple kinases in the interferon-gamma response.
Proc Natl Acad Sci U S A. 105: 6051-6. -
Grotzke, J.E. et al. (2009) The Mycobacterium tuberculosis phagosome is a HLA-I processing competent organelle.
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Koch, N. et al. (2009) IL-10 protects monocytes and macrophages from complement-mediated lysis.
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Arthritis Res Ther. 12: R52. -
Hinrichs, J. et al. (2010) The nature of peptides presented by an HLA class I low expression allele.
Haematologica. 95: 1373-80. -
Fujita, Y. et al. (2010) Bone marrow transplantation restores epidermal basement membrane protein expression and rescues epidermolysis bullosa model mice.
Proc Natl Acad Sci U S A. 107: 14345-50. -
Narita, M. et al. (2010) WT1 peptide vaccination in combination with imatinib therapy for a patient with CML in the chronic phase.
Int J Med Sci. 7: 72-81. -
Zuo, J. et al. (2011) The Epstein-Barr virus-encoded BILF1 protein modulates immune recognition of endogenously processed antigen by targeting major histocompatibility complex class I molecules trafficking on both the exocytic and endocytic pathways.
J Virol. 85: 1604-14. -
Lask, A. et al. (2011) TCR-Independent Killing of B Cell Malignancies by Anti-Third-Party CTLs: The Critical Role of MHC-CD8 Engagement.
J Immunol. 187: 2006-14. -
Jones, D.C. et al. (2011) HLA Class I Allelic Sequence and Conformation Regulate Leukocyte Ig-Like Receptor Binding.
J Immunol. 186: 2990-7. -
Enose-Akahata, Y. et al. (2012) Minocycline modulates antigen-specific CTL activity through inactivation of mononuclear phagocytes in patients with HTLV-I associated neurologic disease.
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Badrinath, S. et al. (2012) Position 156 influences the peptide repertoire and tapasin dependency of human leukocyte antigen B*44 allotypes.
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Tannetta, D.S. et al. (2013) Characterisation of syncytiotrophoblast vesicles in normal pregnancy and pre-eclampsia: expression of Flt-1 and endoglin.
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Dragovic, R.A. et al. (2015) Isolation of syncytiotrophoblast microvesicles and exosomes and their characterisation by multicolour flow cytometry and fluorescence Nanoparticle Tracking Analysis.
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Tischer, S. et al. (2016) Discovery of immunodominant T-cell epitopes reveals penton protein as a second immunodominant target in human adenovirus infection.
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Praest, P. et al. (2019) A Flow Cytometry-Based Approach to Unravel Viral Interference with the MHC Class I Antigen Processing and Presentation Pathway.
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Juan, C.H. et al. (2020) In Vitro Differentiation of Human Placenta-Derived Multipotent Cells into Schwann-Like Cells.
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Nguyen, J. et al. (2021) Quantitative contributions of TNF receptor superfamily members to CD8+ T-cell responses.
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Korbonits, L. et al. (2022) Mycobacterium avium subsp. paratuberculosis Infected Cows Reveal Divergent Immune Response in Bovine Peripheral Blood Derived Lymphocyte Proteome.
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Lyssy, F. et al. (2023) Platelet-derived factors dysregulate placental sphingosine-1-phosphate receptor 2 in human trophoblasts.
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Forstner, D. et al. (2023) CD39 abrogates platelet-derived factors induced IL-1β expression in the human placenta.
Front Cell Dev Biol. 11: 1183793. -
Zheng, S. et al. (2022) The SARS-CoV-2 accessory factor ORF7a downregulates MHC class I surface expression.
bioRχiv 30 May [Epub ahead of print. Preprint] -
Fielding, C.A. et al. (2022) SARS-CoV-2 host-shutoff impacts innate NK cell functions, but antibody-dependent NK activity is strongly activated through non-spike antibodies.
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MCA81SBV670
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