HLA ABC antibody | W6/32

Mouse anti Human HLA ABC:StarBright Violet 670

Product Type
Monoclonal Antibody
Clone
W6/32
Isotype
IgG2a
Specificity
HLA ABC

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MCA81SBV670
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Mouse anti Human HLA ABC antibody, clone W6/32 recognizes an antigenic determinant shared among products of the HLA A, B and C loci. Clone W6/32 recognizes a conformational epitope, reacting with HLA class I alpha3 and alpha2 domains. The major histocompatibility complex (MHC) is a cluster of genes that are important in the immune response to infections. In humans, this complex is referred to as the human leukocyte antigen (HLA) region. There are 3 major MHC class I proteins encoded by the HLA which are HLA A, HLA B and HLA C. These proteins are found on the surface of almost all nucleated somatic cells.

Mouse anti Human HLA ABC antibody, clone W6/32 is routinely tested in flow cytometry on human peripheral blood lymphocytes.

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Macaque
Bovine
Cynomolgus monkey
Baboon
Rhesus Monkey
Chimpanzee
Gorilla
Shrew
Goat
Dog
Guinea Pig
Rabbit
Mouse
Chicken
Amphibia
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG conjugated to StarBright Violet 670 - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
0.1% Pluronic F68
0.1% PEG 3350
0.05% Tween 20
Immunogen
Purified human tonsil lymphocyte membranes.
Fusion Partners
Spleen cells from immunized BALB/c mice were fused with cells of the mouse NS1/1-Ag4.1 myeloma cell line.
Max Ex/Em
Fluorophore Excitation Max (nm) Emission Max (nm)
StarBright Violet 670 400 667
Regulatory
For research purposes only
Guarantee
12 months from date of despatch
Acknowledgements
This product is covered by U.S. Patent No. 10,150,841 and related U.S. and foreign counterparts

Store at +4°C. DO NOT FREEZE.
This product should be stored undiluted.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 5ul of the suggested working dilution to label 106 cells in 100ul. Best practices suggest a 5 minutes centrifugation at 6,000g prior to sample application.

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References for HLA ABC antibody

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  9. View The Latest Product References
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    J Infect Dis. 201: 720-9.
  24. Fujita, Y. et al. (2010) Bone marrow transplantation restores epidermal basement membrane protein expression and rescues epidermolysis bullosa model mice.
    Proc Natl Acad Sci U S A. 107: 14345-50.
  25. Grotzke, J.E. et al. (2009) The Mycobacterium tuberculosis phagosome is a HLA-I processing competent organelle.
    PLoS Pathog. 5: e1000374.
  26. Narita, M. et al. (2010) WT1 peptide vaccination in combination with imatinib therapy for a patient with CML in the chronic phase.
    Int J Med Sci. 7: 72-81.
  27. Vitadello, M. et al. (2010) Myofiber stress-response in myositis: parallel investigations on patients and experimental animal models of muscle regeneration and systemic inflammation.
    Arthritis Res Ther. 12: R52.
  28. Zuo, J. et al. (2011) The Epstein-Barr virus-encoded BILF1 protein modulates immune recognition of endogenously processed antigen by targeting major histocompatibility complex class I molecules trafficking on both the exocytic and endocytic pathways.
    J Virol. 85: 1604-14.
  29. Enose-Akahata, Y. et al. (2012) Minocycline modulates antigen-specific CTL activity through inactivation of mononuclear phagocytes in patients with HTLV-I associated neurologic disease.
    Retrovirology. 9: 16.
  30. Badrinath, S. et al. (2012) Position 156 influences the peptide repertoire and tapasin dependency of human leukocyte antigen B*44 allotypes.
    Haematologica. 97: 98-106.
  31. Tischer, S. et al. (2016) Discovery of immunodominant T-cell epitopes reveals penton protein as a second immunodominant target in human adenovirus infection.
    J Transl Med. 14 (1): 286.
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    Methods Mol Biol. 1988: 187-98.
  34. Tannetta, D.S. et al. (2013) Characterisation of syncytiotrophoblast vesicles in normal pregnancy and pre-eclampsia: expression of Flt-1 and endoglin.
    PLoS One. 8 (2): e56754.
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    Biomolecules. 10 (12) Dec 10 [Epub ahead of print].
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    Mol Syst Biol. 17 (11): e10560.
  38. Cacciola, R. et al. (2022) Impact of Anti-Endothelial Cell Antibodies (AECAs) in Patients with Polycythemia Vera and Thrombosis
    Diagnostics. 12 (5): 1077.
  39. Xia, P. et al. (2022) NLRP3 inflammasome up-regulates major histocompatibility complex class I expression and promotes inflammatory infiltration in polymyositis.
    BMC Immunol. 23 (1): 39.

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