Rabbit anti Human histone H3F3A (pThr11) antibody
recognizes histone H3.3, when phosphorylated at threonine 11. In eukaryotic cells, histones are proteins which associate with nuclear DNA and help condense it into chromatin, playing important roles in gene regulation. H3F3A is a common non-centromeric variant of histone H3, and appears to play a role in both gene activation and silencing (Shi et al. 2017
). Post-translational modifications of histones alter the interactions between histones and DNA, and between histones and other proteins, with dramatic consequences for DNA transcription and other biological events (Sawika and Seiser 2012
). This protein can undergo massive phosphorylation at threonine 11, among other sites, altering the function of histone H3 in a temporal and spatial way. This antibody detects H3F3A when phosphorylated at threonine 11.
- Target Species
- Species Cross-Reactivity
|Target Species||Cross Reactivity|
||Expected from Sequence
||Expected from Sequence
- N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Purified IgG prepared by affinity chromatography of Protein A from animal origin-free culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide
1% Bovine Serum Albumin
- A phospho-peptide corresponding to H3F3A (pThr11)
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Store at -20°C only.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use
- 12 months from date of despatch
- Entrez Gene
- GO Terms
- For research purposes only
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Applications of Histone H3F3A (pThr11) antibody
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
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Secondary Antibodies Available
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