Histone H3F3A (pThr11) antibody | RM164

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Rabbit anti Histone H3F3A (pThr11)

Product Type
Monoclonal Antibody
Clone
RM164
Isotype
IgG
Product Code Applications Pack Size List Price Quantity
MCA6296
Datasheet
E WB
SDS
0.1 mg loader

Rabbit anti Human histone H3F3A (pThr11) antibody recognizes histone H3.3, when phosphorylated at threonine 11. In eukaryotic cells, histones are proteins which associate with nuclear DNA and help condense it into chromatin, playing important roles in gene regulation. H3F3A is a common non-centromeric variant of histone H3, and appears to play a role in both gene activation and silencing (Shi et al. 2017). Post-translational modifications of histones alter the interactions between histones and DNA, and between histones and other proteins, with dramatic consequences for DNA transcription and other biological events (Sawika and Seiser 2012). This protein can undergo massive phosphorylation at threonine 11, among other sites, altering the function of histone H3 in a temporal and spatial way. This antibody detects H3F3A when phosphorylated at threonine 11.

Product Details

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse Expected from Sequence
Rat Expected from Sequence
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography of Protein A from animal origin-free culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide
1% Bovine Serum Albumin
50% Glycerol
Immunogen
A phospho-peptide corresponding to H3F3A (pThr11)
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml

Storage Information

Storage
Store at -20°C only.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use
Guarantee
12 months from date of despatch

More Information

UniProt
P84243
Entrez Gene
H3F3B
GO Terms
GO:0000786 nucleosome
GO:0003677 DNA binding
GO:0006334 nucleosome assembly
GO:0007596 blood coagulation
GO:0005654 nucleoplasm
Regulatory
For research purposes only

Applications of Histone H3F3A (pThr11) antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1 μg/ml 0.2 μg/ml
Western Blotting 2 μg/ml 0.5 μg/ml
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Rabbit IgG (Fc):Biotin STAR121B E WB 1 mg loader
Goat anti Rabbit IgG (Fc):FITC STAR121F F 1 mg loader
Goat anti Rabbit IgG (Fc):HRP STAR121P E WB 1 mg loader
Sheep anti Rabbit IgG:FITC STAR34B C F 1 mg loader
Sheep anti Rabbit IgG:DyLight®488 STAR36D488GA F IF WB 0.1 mg
Sheep anti Rabbit IgG:DyLight®680 STAR36D680GA F IF WB 0.1 mg
Sheep anti Rabbit IgG:Dylight®800 STAR36D800GA F IF WB 0.1 mg
Goat anti Rabbit IgG (H/L):HRP STAR124P C E WB 1 mg loader
Sheep anti Rabbit IgG:RPE STAR35A F 1 ml loader

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