Histone H3F3A (pSer10) antibody | RM163
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WB IC ResetRabbit anti Histone H3F3A (pSer10)
- Product Type
- Monoclonal Antibody
- Clone
- RM163
- Isotype
- IgG
- Specificity
- Histone H3F3A (pSer10)
Antibody Quality and Performance Guarantee
| Rabbit anti Human histone H3F3A (pSer10) antibody recognizes histone H3.3, when phosphorylated at serine 10. In eukaryotic cells, histones are proteins which associate with nuclear DNA and help condense it into chromatin, playing important roles in gene regulation. H3F3A is a common non-centromeric variant of histone H3, and appears to play a role in both gene activation and silencing (Shi et al. 2017). Post-translational modifications of histones alter the interactions between histones and DNA, and between histones and other proteins, with dramatic consequences for DNA transcription and other biological events (Sawika and Seiser 2012). This protein can undergo massive phosphorylation at serine 10, among other sites, altering the function of histone H3 in a temporal and spatial way. This antibody detects H3F3A when phosphorylated at serine 10. |
- Target Species
- Human
- Species Cross-Reactivity
-
Target Species Cross Reactivity Mouse Expected from Sequence Rat Expected from Sequence - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography of Protein A from animal origin-free culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide
1% Bovine Serum Albumin
50% Glycerol - Immunogen
- A phospho-peptide corresponding to H3F3A (pSer10)
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
Store at -20°C only.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |  |
1 μg/ml | 0.2 μg/ml |
| Immunocytochemistry | |
2 μg/ml | 0.5 μg/ml |
| Western Blotting | |
2 μg/ml | 0.5 μg/ml |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
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- UniProt
- P84243
- Entrez Gene
- H3F3B
- GO Terms
- GO:0000786 nucleosome
- GO:0003677 DNA binding
- GO:0006334 nucleosome assembly
- GO:0007596 blood coagulation
- GO:0005654 nucleoplasm
MCA6293
155766
If you cannot find the batch/lot you are looking for please contact our technical support team for assistance.
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"
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