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Histone H3F3A (pSer10) antibody | RM163

Rabbit anti Histone H3F3A (pSer10)

Product Type
Monoclonal Antibody
Clone
RM163
Isotype
IgG
Specificity
Histone H3F3A (pSer10)

Product Code Applications Pack Size List Price Your Price Qty
MCA6293
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
E IC WB 0.1 mg loader
List Price Your Price
loader

Rabbit anti Human histone H3F3A (pSer10) antibody recognizes histone H3.3, when phosphorylated at serine 10. In eukaryotic cells, histones are proteins which associate with nuclear DNA and help condense it into chromatin, playing important roles in gene regulation. H3F3A is a common non-centromeric variant of histone H3, and appears to play a role in both gene activation and silencing (Shi et al. 2017). Post-translational modifications of histones alter the interactions between histones and DNA, and between histones and other proteins, with dramatic consequences for DNA transcription and other biological events (Sawika and Seiser 2012). This protein can undergo massive phosphorylation at serine 10, among other sites, altering the function of histone H3 in a temporal and spatial way. This antibody detects H3F3A when phosphorylated at serine 10.

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse Expected from Sequence
Rat Expected from Sequence
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography of Protein A from animal origin-free culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide
1% Bovine Serum Albumin
50% Glycerol
Immunogen
A phospho-peptide corresponding to H3F3A (pSer10)
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

Store at -20°C only.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1 μg/ml 0.2 μg/ml
Immunocytochemistry 2 μg/ml 0.5 μg/ml
Western Blotting 2 μg/ml 0.5 μg/ml
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

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UniProt
P84243
Entrez Gene
H3F3B
GO Terms
GO:0000786 nucleosome
GO:0003677 DNA binding
GO:0006334 nucleosome assembly
GO:0007596 blood coagulation
GO:0005654 nucleoplasm

MCA6293

155766

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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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