Histone H3F3A (pSer10) antibody | RM163
Rabbit anti Human histone H3F3A (pSer10) antibody recognizes histone H3.3, when phosphorylated at serine 10. In eukaryotic cells, histones are proteins which associate with nuclear DNA and help condense it into chromatin, playing important roles in gene regulation. H3F3A is a common non-centromeric variant of histone H3, and appears to play a role in both gene activation and silencing (Shi et al. 2017). Post-translational modifications of histones alter the interactions between histones and DNA, and between histones and other proteins, with dramatic consequences for DNA transcription and other biological events (Sawika and Seiser 2012). This protein can undergo massive phosphorylation at serine 10, among other sites, altering the function of histone H3 in a temporal and spatial way. This antibody detects H3F3A when phosphorylated at serine 10.
Product Details
- Target Species
- Human
- Species Cross-Reactivity
-
Target Species Cross Reactivity Mouse Expected from Sequence Rat Expected from Sequence - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography of Protein A from animal origin-free culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide
1% Bovine Serum Albumin
50% Glycerol - Immunogen
- A phospho-peptide corresponding to H3F3A (pSer10)
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
Storage Information
- Storage
- Store at -20°C only.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use - Guarantee
- 12 months from date of despatch
More Information
- UniProt
- P84243
- Entrez Gene
- H3F3B
- GO Terms
- GO:0000786 nucleosome
- GO:0003677 DNA binding
- GO:0006334 nucleosome assembly
- GO:0007596 blood coagulation
- GO:0005654 nucleoplasm
- Regulatory
- For research purposes only
Applications of Histone H3F3A (pSer10) antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA | 1 μg/ml | 0.2 μg/ml | |
| Immunocytochemistry | 2 μg/ml | 0.5 μg/ml | |
| Western Blotting | 2 μg/ml | 0.5 μg/ml |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
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