Histone H2A/H4 (pSer1) antibody | RM216
Rabbit anti Histone H2A/H4 (pSer1)
- Product Type
- Monoclonal Antibody
- Clone
- RM216
- Isotype
- IgG
- Specificity
- Histone H2A/H4 (pSer1)
Antibody Quality and Performance Guarantee
| Rabbit anti Histone H2A/H4 (pSer1) antibody recognizes Histone H2AX, and Histone H4 when phosphorylated at serine 1. In eukaryotic cells, histones are proteins which associate with nuclear DNA and help condense it into chromatin, playing important roles in gene regulation. Histone H2AX is a member of the histone 2A (H2A) family, which constitutes 2 to 25% of mammalian histone H2A (Dickey et al. 2009). H2AX involved with nucleosomal organization of chromatin. H2AX can act as a tumor suppressor that plays key roles in repair of DNA double-strand breaks and genome stability during mitotic and meiotic division (Weyemi et al. 2016). The role of H2AX in chromatin remodeling also extends to stem cell development, and to maintenance of cellular senescence (Turinetto and Giachino 2015). Like histone H2A, histone 4 (H4) is a core histone involved with condensing chromatin (Dai et al. 2008). Histone H4 is phosphorylated at serine 1 during DNA damage, and is localized to the double-strand break (DSB). This phosphorylation is mediated by CK2, and appears to function in DSB repair (Cheung et al. 2005). |
- Target Species
- Human
- Species Cross-Reactivity
-
Target Species Cross Reactivity Mouse Expected from Sequence Rat Expected from Sequence - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Preparation
- Protein A affinity purified from an animal origin-free culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide
1% Bovine Serum Albumin
50% Glycerol - Immunogen
- A phospho-peptide corresponding to Phospho-Histone H2A (pSer1)
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
Store at -20°C only.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |  |
1 μg/ml | 0.2 μg/ml |
| Immunocytochemistry | |
2 μg/ml | 1 μg/ml |
| Western Blotting | |
2 μg/ml | 0.5 μg/ml |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
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- UniProt
- P16104
- P62805
- Entrez Gene
- H2AFX
- HIST2H4B
- GO Terms
- GO:0000077 DNA damage checkpoint
- GO:0000724 double-strand break repair via homologous recombination
- GO:0000786 nucleosome
- GO:0006334 nucleosome assembly
- GO:0042393 histone binding
- GO:0005654 nucleoplasm
- GO:0007126 meiosis
- GO:0010212 response to ionizing radiation
- GO:0019899 enzyme binding
- View More GO Terms
- GO:0045739 positive regulation of DNA repair
- GO:0005515 protein binding
- GO:0000723 telomere maintenance
- GO:0003677 DNA binding
- GO:0048015 phosphatidylinositol-mediated signaling
- GO:0034080 CenH3-containing nucleosome assembly at centromere
- GO:0045653 negative regulation of megakaryocyte differentiation
MCA6292
155765
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