Rabbit anti Histone H2A/H4 (pSer1) antibody
recognizes Histone H2AX, and Histone H4 when phosphorylated at serine 1. In eukaryotic cells, histones are proteins which associate with nuclear DNA and help condense it into chromatin, playing important roles in gene regulation. Histone H2AX is a member of the histone 2A (H2A) family, which constitutes 2 to 25% of mammalian histone H2A (Dickey et al. 2009
). H2AX involved with nucleosomal organization of chromatin. H2AX can act as a tumor suppressor that plays key roles in repair of DNA double-strand breaks and genome stability during mitotic and meiotic division (Weyemi et al. 2016
). The role of H2AX in chromatin remodeling also extends to stem cell development, and to maintenance of cellular senescence (Turinetto and Giachino 2015
). Like histone H2A, histone 4 (H4) is a core histone involved with condensing chromatin (Dai et al. 2008
). Histone H4 is phosphorylated at serine 1 during DNA damage, and is localized to the double-strand break (DSB). This phosphorylation is mediated by CK2, and appears to function in DSB repair (Cheung et al. 2005
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Applications of Histone H2A/H4 (pSer1) antibody
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.