Histone H2A/H4 (pSer1) antibody | RM216

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Rabbit anti Histone H2A/H4 (pSer1)

Product Type
Monoclonal Antibody
Clone
RM216
Isotype
IgG
Product Code Applications Pack Size List Price Quantity
MCA6292
Datasheet
E IC WB
SDS
0.1 mg loader

Rabbit anti Histone H2A/H4 (pSer1) antibody recognizes Histone H2AX, and Histone H4 when phosphorylated at serine 1. In eukaryotic cells, histones are proteins which associate with nuclear DNA and help condense it into chromatin, playing important roles in gene regulation. Histone H2AX is a member of the histone 2A (H2A) family, which constitutes 2 to 25% of mammalian histone H2A (Dickey et al. 2009). H2AX involved with nucleosomal organization of chromatin. H2AX can act as a tumor suppressor that plays key roles in repair of DNA double-strand breaks and genome stability during mitotic and meiotic division (Weyemi et al. 2016). The role of H2AX in chromatin remodeling also extends to stem cell development, and to maintenance of cellular senescence (Turinetto and Giachino 2015). Like histone H2A, histone 4 (H4) is a core histone involved with condensing chromatin (Dai et al. 2008). Histone H4 is phosphorylated at serine 1 during DNA damage, and is localized to the double-strand break (DSB). This phosphorylation is mediated by CK2, and appears to function in DSB repair (Cheung et al. 2005).

Product Details

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse Expected from Sequence
Rat Expected from Sequence
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Protein A affinity purified from an animal origin-free culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide
1% Bovine Serum Albumin
50% Glycerol
Immunogen
A phospho-peptide corresponding to Phospho-Histone H2A (pSer1)
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml

Storage Information

Storage
Store at -20°C only.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
12 months from date of despatch

More Information

UniProt
P16104
P62805
Entrez Gene
H2AFX
HIST2H4B
GO Terms
GO:0000077 DNA damage checkpoint
GO:0000724 double-strand break repair via homologous recombination
GO:0000786 nucleosome
GO:0006334 nucleosome assembly
GO:0042393 histone binding
GO:0005654 nucleoplasm
GO:0007126 meiosis
GO:0010212 response to ionizing radiation
GO:0019899 enzyme binding
GO:0045739 positive regulation of DNA repair
GO:0005515 protein binding
GO:0000723 telomere maintenance
GO:0003677 DNA binding
GO:0048015 phosphatidylinositol-mediated signaling
GO:0034080 CenH3-containing nucleosome assembly at centromere
GO:0045653 negative regulation of megakaryocyte differentiation
Regulatory
For research purposes only

Applications of Histone H2A/H4 (pSer1) antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1 μg/ml 0.2 μg/ml
Immunocytochemistry 2 μg/ml 1 μg/ml
Western Blotting 2 μg/ml 0.5 μg/ml
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Rabbit IgG (Fc):Biotin STAR121B E WB 1 mg loader
Goat anti Rabbit IgG (Fc):FITC STAR121F F 1 mg loader
Goat anti Rabbit IgG (Fc):HRP STAR121P E WB 1 mg loader
Sheep anti Rabbit IgG:FITC STAR34B C F 1 mg loader
Sheep anti Rabbit IgG:DyLight®488 STAR36D488GA F IF WB 0.1 mg
Sheep anti Rabbit IgG:DyLight®680 STAR36D680GA F IF WB 0.1 mg
Sheep anti Rabbit IgG:Dylight®800 STAR36D800GA F IF WB 0.1 mg
Goat anti Rabbit IgG (H/L):HRP STAR124P C E WB 1 mg loader
Sheep anti Rabbit IgG:RPE STAR35A F 1 ml loader

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