Rabbit anti Histone H2A/H4 (pSer1) antibody recognizes Histone H2AX, and Histone H4 when phosphorylated at serine 1. In eukaryotic cells, histones are proteins which associate with nuclear DNA and help condense it into chromatin, playing important roles in gene regulation. Histone H2AX is a member of the histone 2A (H2A) family, which constitutes 2 to 25% of mammalian histone H2A (Dickey et al. 2009). H2AX involved with nucleosomal organization of chromatin. H2AX can act as a tumor suppressor that plays key roles in repair of DNA double-strand breaks and genome stability during mitotic and meiotic division (Weyemi et al. 2016). The role of H2AX in chromatin remodeling also extends to stem cell development, and to maintenance of cellular senescence (Turinetto and Giachino 2015). Like histone H2A, histone 4 (H4) is a core histone involved with condensing chromatin (Dai et al. 2008). Histone H4 is phosphorylated at serine 1 during DNA damage, and is localized to the double-strand break (DSB). This phosphorylation is mediated by CK2, and appears to function in DSB repair (Cheung et al. 2005).
- Target Species
- Species Cross-Reactivity
|Target Species||Cross Reactivity|
||Expected from Sequence
||Expected from Sequence
- N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Protein A affinity purified from an animal origin-free culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide
1% Bovine Serum Albumin
- A phospho-peptide corresponding to Phospho-Histone H2A (pSer1)
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Store at -20°C only.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.
- 12 months from date of despatch
- Entrez Gene
- GO Terms
DNA damage checkpoint
double-strand break repair via homologous recombination
response to ionizing radiation
positive regulation of DNA repair
CenH3-containing nucleosome assembly at centromere
negative regulation of megakaryocyte differentiation
- For research purposes only
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Applications of Histone H2A/H4 (pSer1) antibody
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
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Secondary Antibodies Available
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