HIF2 Alpha antibody

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Anti HIF2 Alpha Antibody (PrecisionAb Monoclonal Antibody) gallery image 1 — Western blot analysis of whole cell lysates probed with Mouse anti Human HIF2 alpha antibody (**VMA00083**) followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000, STAR207P) and visualized on the ChemiDoc MP with 8 second exposure. Arrow points to HIF2 alpha (molecular weight 117 kDa).

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Mouse anti HIF2 Alpha

Product Type: PrecisionAb Monoclonal
Isotype: IgG1
Format: Purified
Specificity: HIF2 Alpha

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Product Code Applications Pack Size List Price Your Price Qty

List Price	Your Price
VMA00083 Datasheet Datasheet SDS SDS	C WB	100 µl		Log in	Get Quote
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Mouse anti Human HIF2 alpha antibody recognizes HIF2 alpha, also known as EPAS-1, basic-helix-loop-helix-PAS protein MOP2, class E basic helix-loop-helix protein 73, bHLHe73, HIF-1-alpha-like factor, HLF, hypoxia-inducible factor 2-alpha, HIF-2-alpha, HIF2-alpha, member of PAS protein 2 and PAS domain-containing protein 2.

HIF 2 alpha is a transcription factor involved in the induction of genes regulated by oxygen, which is induced as oxygen levels fall. Encoded by the EPAS1 gene, HIF2 alpha contains a basic-helix-loop-helix domain protein dimerization domain as well as a domain found in proteins in signal transduction pathways which respond to oxygen levels. Mutations in HIF2 alpha are associated with erythrocytosis familial type 4 (provided by RefSeq, Nov 2009).

Mouse anti Human HIF2 alpha antibody detects a band of 117 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Target Species: Human
Western Blotting: Anti HIF2 alpha detects a band of approximately 117 kDa in HeLa cell lysates.
Species Cross-Reactivity: Target Species Cross Reactivity

Mouse

Rat; N.B. Antibody reactivity and working conditions may vary between species.
Product Form: Purified IgG - liquid
Preparation: Mouse monoclonal antibody purified by affinity chromatography on Protein G from tissue culture supernatant.
Buffer Solution: Phosphate buffered saline.
Preservative Stabilisers: 0.09% Sodium Azide (NaN₃)
1% Bovine Serum Albumin
<50% Glycerol.
Immunogen: Recombinant protein corresponding to an internal region of human HIF2 alpha
Regulatory: For research purposes only.
Guarantee: 12 months from date of despatch
Acknowledgements: PrecisionAb is a trademark of Bio-Rad Laboratories.

Target Species	Cross Reactivity
Mouse
Rat

Store undiluted at -20°C, avoiding repeated freeze thaw cycles.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Immunohistology - Frozen
Western Blotting		1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Description	Product Code	Applications	Pack Size	Your Price
Goat anti Mouse IgG (H/L):HRP	STAR207P	WB	2 ml	Log in	Get Quote
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Description	Goat anti Mouse IgG (H/L):HRP

UniProt: Q99814
Entrez Gene: EPAS1
GO Terms: GO:0001525 angiogenesis; GO:0001666 response to hypoxia; GO:0003705 sequence-specific enhancer binding RNA polymerase II transcription factor activity; GO:0003713 transcription coactivator activity; GO:0004871 signal transducer activity; GO:0005667 transcription factor complex; GO:0008134 transcription factor binding; GO:0035035 histone acetyltransferase binding; GO:0045944 positive regulation of transcription from RNA polymerase II promoter; View More GO Terms; GO:0046982 protein heterodimerization activity; GO:0048625 myoblast cell fate commitment