HIF2 Alpha antibody

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Mouse anti HIF2 Alpha

HIF2 alpha antibody recognizes endothelial PAS domain-containing protein 1, also known as EPAS-1, HIF-1-alpha-like factor, PAS domain-containing protein 2, basic-helix-loop-helix-PAS protein MOP2, or member of PAS protein 2.

HIF2 Alpha Antibody with Control Lysate

HIF2 alpha antibody recognizes endothelial PAS domain-containing protein 1, also known as EPAS-1, HIF-1-alpha-like factor, PAS domain-containing protein 2, basic-helix-loop-helix-PAS protein MOP2, or class E basic helix-loop-helix protein 73.

Product Type
PrecisionAb™ Monoclonal
Isotype
IgG1
Format
Purified

Select your package type:

Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
VMA00083 WB 100 µl
VMA00083KT WB 2 Westerns
With Lysate 400µg
Mouse anti Human HIF2 alpha antibody recognizes HIF2 alpha, also known as EPAS-1, basic-helix-loop-helix-PAS protein MOP2, class E basic helix-loop-helix protein 73, bHLHe73, HIF-1-alpha-like factor, HLF, hypoxia-inducible factor 2-alpha, HIF-2-alpha, HIF2-alpha, member of PAS protein 2 and PAS domain-containing protein 2.

HIF 2 alpha is a transcription factor involved in the induction of genes regulated by oxygen, which is induced as oxygen levels fall. Encoded by the EPAS1 gene, HIF2 alpha contains a basic-helix-loop-helix domain protein dimerization domain as well as a domain found in proteins in signal transduction pathways which respond to oxygen levels. Mutations in HIF2 alpha are associated with erythrocytosis familial type 4 (provided by RefSeq, Nov 2009).

Mouse anti Human HIF2 alpha antibody detects a band of 117 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species
Human
Western Blotting
Anti HIF2 alpha detects a band of approximately 117 kDa in HeLa cell lysates.
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Product Form
Purified IgG - liquid
Preparation
Mouse monoclonal antibody purified by affinity chromatography on Protein G from tissue culture supernatant
Preparation
20μl Mouse monoclonal antibody purified by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
<50% Glycerol
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
<50% Glycerol
Immunogen
Recombinant protein corresponding to an internal region of human HIF2 alpha
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.

Positive Control Lysate

Lysate Composition
400μg HeLa lysate lyophilized in RIPA buffer.
Lysate Reconstitution
- If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
- If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Storage
Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
Shelf Life
As supplied, 12 months from date of despatch.
Shelf Life
As supplied, 12 months from date of despatch.

More Information

UniProt
Q99814 Related reagents
Entrez Gene
EPAS1 Related reagents
GO Terms
GO:0001525 angiogenesis
GO:0001666 response to hypoxia
GO:0003705 sequence-specific enhancer binding RNA polymerase II transcription factor activity
GO:0003713 transcription coactivator activity
GO:0004871 signal transducer activity
GO:0005667 transcription factor complex
GO:0008134 transcription factor binding
GO:0035035 histone acetyltransferase binding
GO:0045944 positive regulation of transcription from RNA polymerase II promoter
GO:0046982 protein heterodimerization activity
GO:0048625 myoblast cell fate commitment
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Regulatory
For research purposes only

Applications of HIF2 Alpha antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000
Western Blotting 1/1000

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Goat anti Mouse IgG (H/L):HRP STAR207P 2 ml WB
Goat anti Mouse IgG (H/L):HRP STAR207P 2 ml WB

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