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HIF2 Alpha antibody

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Western Blot Validated
Anti HIF2 Alpha Antibody (PrecisionAb Monoclonal Antibody) gallery image 1
Western blot analysis of whole cell lysates probed with HIF2 alpha antibody (VMA00083) followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000, STAR207P) and visualized on the ChemiDoc MP with 8 second exposure. Arrow points to HIF2 alpha (molecular weight 117 kDa).

Mouse anti HIF2 Alpha

Product Type
PrecisionAb Monoclonal
Isotype
IgG1
Format
Purified
PrecisionAb Antibodies
Product Code Applications Pack Size List Price Quantity
VMA00083
Datasheet
C WB
SDS
100 µl loader

Mouse anti Human HIF2 alpha antibody recognizes HIF2 alpha, also known as EPAS-1, basic-helix-loop-helix-PAS protein MOP2, class E basic helix-loop-helix protein 73, bHLHe73, HIF-1-alpha-like factor, HLF, hypoxia-inducible factor 2-alpha, HIF-2-alpha, HIF2-alpha, member of PAS protein 2 and PAS domain-containing protein 2.

HIF 2 alpha is a transcription factor involved in the induction of genes regulated by oxygen, which is induced as oxygen levels fall. Encoded by the EPAS1 gene, HIF2 alpha contains a basic-helix-loop-helix domain protein dimerization domain as well as a domain found in proteins in signal transduction pathways which respond to oxygen levels. Mutations in HIF2 alpha are associated with erythrocytosis familial type 4 (provided by RefSeq, Nov 2009).

Mouse anti Human HIF2 alpha antibody detects a band of 117 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species
Human
Western Blotting
Anti HIF2 alpha detects a band of approximately 117 kDa in HeLa cell lysates.
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Mouse monoclonal antibody purified by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
<50% Glycerol
Immunogen
Recombinant protein corresponding to an internal region of human HIF2 alpha

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Guarantee
12 months from date of despatch

More Information

UniProt
Q99814
Entrez Gene
EPAS1
GO Terms
GO:0001525 angiogenesis
GO:0001666 response to hypoxia
GO:0003705 sequence-specific enhancer binding RNA polymerase II transcription factor activity
GO:0003713 transcription coactivator activity
GO:0004871 signal transducer activity
GO:0005667 transcription factor complex
GO:0008134 transcription factor binding
GO:0035035 histone acetyltransferase binding
GO:0045944 positive regulation of transcription from RNA polymerase II promoter
GO:0046982 protein heterodimerization activity
GO:0048625 myoblast cell fate commitment
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories.
Regulatory
For research purposes only

Applications of HIF2 Alpha antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunohistology - Frozen
Western Blotting 1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.
Copyright © 2021 Bio-Rad Antibodies (formerly AbD Serotec)

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Mouse IgG (H/L):HRP STAR207P WB 2 ml

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