HIF2 Alpha antibody
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WB ResetMouse anti HIF2 Alpha
- Product Type
- PrecisionAb Monoclonal
- Isotype
- IgG1
- Format
- Purified
- Specificity
- HIF2 Alpha
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| Mouse anti Human HIF2 alpha antibody recognizes HIF2 alpha, also known as EPAS-1, basic-helix-loop-helix-PAS protein MOP2, class E basic helix-loop-helix protein 73, bHLHe73, HIF-1-alpha-like factor, HLF, hypoxia-inducible factor 2-alpha, HIF-2-alpha, HIF2-alpha, member of PAS protein 2 and PAS domain-containing protein 2. HIF 2 alpha is a transcription factor involved in the induction of genes regulated by oxygen, which is induced as oxygen levels fall. Encoded by the EPAS1 gene, HIF2 alpha contains a basic-helix-loop-helix domain protein dimerization domain as well as a domain found in proteins in signal transduction pathways which respond to oxygen levels. Mutations in HIF2 alpha are associated with erythrocytosis familial type 4 (provided by RefSeq, Nov 2009). Mouse anti Human HIF2 alpha antibody detects a band of 117 kDa. The antibody has been extensively validated for western blotting using whole cell lysates. |
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- Target Species
- Human
- Western Blotting
- Anti HIF2 alpha detects a band of approximately 117 kDa in HeLa cell lysates.
- Species Cross-Reactivity
-
Target Species Cross Reactivity Mouse Rat - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Preparation
- Mouse monoclonal antibody purified by affinity chromatography on Protein G from tissue culture supernatant.
- Buffer Solution
- Phosphate buffered saline.
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
<50% Glycerol. - Immunogen
- Recombinant protein corresponding to an internal region of human HIF2 alpha
- Regulatory
- For research purposes only.
- Guarantee
- 12 months from date of despatch
- Acknowledgements
- PrecisionAb is a trademark of Bio-Rad Laboratories.
Store undiluted at -20°C, avoiding repeated freeze thaw cycles.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Immunohistology - Frozen |  |
||
| Western Blotting | |
1/1000 |
The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Goat anti Mouse IgG (H/L):HRP | STAR207P | WB | 2 ml |
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| List Price | Your Price | ||||||
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| Description | Goat anti Mouse IgG (H/L):HRP | ||||||
- UniProt
- Q99814
- Entrez Gene
- EPAS1
- GO Terms
- GO:0001525 angiogenesis
- GO:0001666 response to hypoxia
- GO:0003705 sequence-specific enhancer binding RNA polymerase II transcription factor activity
- GO:0003713 transcription coactivator activity
- GO:0004871 signal transducer activity
- GO:0005667 transcription factor complex
- GO:0008134 transcription factor binding
- GO:0035035 histone acetyltransferase binding
- GO:0045944 positive regulation of transcription from RNA polymerase II promoter
- View More GO Terms
- GO:0046982 protein heterodimerization activity
- GO:0048625 myoblast cell fate commitment
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