HIF1 Alpha antibody | RM242
Rabbit anti HIF1 Alpha
- Product Type
- Monoclonal Antibody
- Clone
- RM242
- Isotype
- IgG
- Specificity
- HIF1 Alpha
Rabbit anti Human HIF1 alpha antibody recognizes hypoxia-inducible factor 1-alpha, also known as BHLHE78, MOP1. HIF transcription factors are the main regulators of cellular responses to hypoxia (low oxygen levels), promoting cell survival. HIF is a heterodimer consisting of either HIF1 alpha, HIF2 alpha, or HIF3 alpha bound to HIF 1 beta (Huang et al. 2017). Under normal oxygen levels HIF1 alpha is degraded via the ubiquitin-dependent pathway, but under hypoxia, HIF 1 alpha is stablized, leading to HIF1 binding to the hypoxia responsive element (HRE) in the nucleus and activating genes involved with angiogenesis, migration and survival (Tatrai et al. 2017). HIF1 alpha expression is elevated in several human cancers including glioblastoma multiforme, hemangioblastoma, and colonic adenocarcinoma, and is strongly associated with mortality and increased metastasis. HIF1 alpha upregulation activates many oncogenic processes including angiogenesis, migration and invasion, pH regulation, and glucose metabolism (Balamurugan 2016). |
- Target Species
- Human
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography of Protein A from animal origin-free culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide
1% Bovine Serum Albumin
50% Glycerol - Immunogen
- A peptide corresponding to HIF1 alpha
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use
Application Name | Verified | Min Dilution | Max Dilution |
---|---|---|---|
Immunohistology - Paraffin | 1/500 | 1/1000 | |
Western Blotting | 1/1000 | 1/2000 |
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Goat anti Rabbit IgG (H/L):HRP | STAR124P | C E WB | 1 mg | Log in | |||
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Sheep anti Rabbit IgG:RPE | STAR35A | F | 1 ml | Log in | |||
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Description | Sheep anti Rabbit IgG:RPE |
- UniProt
- Q16665
- Entrez Gene
- HIF1A
- GO Terms
- GO:0001666 response to hypoxia
- GO:0001837 epithelial to mesenchymal transition
- GO:0001938 positive regulation of endothelial cell proliferation
- GO:0002248 connective tissue replacement involved in inflammatory response wound healing
- GO:0003677 DNA binding
- GO:0003705 sequence-specific enhancer binding RNA polymerase II transcription factor activity
- GO:0004871 signal transducer activity
- GO:0005737 cytoplasm
- GO:0005667 transcription factor complex
- View More GO Terms
- GO:0005730 nucleolus
- GO:0008134 transcription factor binding
- GO:0051879 Hsp90 protein binding
- GO:0010575 positive regulation vascular endothelial growth factor production
- GO:0010634 positive regulation of epithelial cell migration
- GO:0046886 positive regulation of hormone biosynthetic process
- GO:0030528 transcription regulator activity
- GO:0030949 positive regulation of vascular endothelial growth factor receptor signaling pathway
- GO:0032364 oxygen homeostasis
- GO:0032722 positive regulation of chemokine production
- GO:0032909 regulation of transforming growth factor-beta2 production
- GO:0032963 collagen metabolic process
- GO:0035035 histone acetyltransferase binding
- GO:0042789 mRNA transcription from RNA polymerase II promoter
- GO:0043193 positive regulation of gene-specific transcription
- GO:0043619 regulation of transcription from RNA polymerase II promoter in response to oxidative stress
- GO:0045648 positive regulation of erythrocyte differentiation
- GO:0045766 positive regulation of angiogenesis
- GO:0045821 positive regulation of glycolysis
- GO:0045944 positive regulation of transcription from RNA polymerase II promoter
- GO:0046982 protein heterodimerization activity
- GO:0051000 positive regulation of nitric-oxide synthase activity
- GO:0051541 elastin metabolic process
MCA6302
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"
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