HIF1 Alpha antibody | RM242

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Rabbit anti HIF1 Alpha

Product Type
Monoclonal Antibody
Clone
RM242
Isotype
IgG
Product Code Applications Pack Size List Price Quantity
MCA6302
Datasheet
P WB
SDS
0.1 ml loader

Rabbit anti Human HIF1 alpha antibody recognizes hypoxia-inducible factor 1-alpha, also known as BHLHE78, MOP1. HIF transcription factors are the main regulators of cellular responses to hypoxia (low oxygen levels), promoting cell survival (Huang et al. 2017). HIF is a heterodimer consisting of either HIF1 alpha, HIF2 alpha, or HIF3 alpha bound to HIF 1 beta (Huang et al. 2018). Under normal oxygen levels HIF1 alpha is degraded via the ubiquitin-dependent pathway, but under hypoxia, HIF 1 alpha is stablized, leading to HIF1 binding to the hypoxia responsive element (HRE) in the nucleus and activating genes involved with angiogenesis, migration and survival (Tatrai et al. 2017). HIF1 alpha expression is elevated in several human cancers including glioblastoma multiforme, hemangioblastoma, and colonic adenocarcinoma, and is strongly associated with mortality and increased metastasis. HIF1 alpha upregulation activates many oncogenic processes including angiogenesis, migration and invasion, pH regulation, and glucose metabolism (Balamurugan 2016).

Product Details

Target Species
Human
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography of Protein A from animal origin-free culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide
1% Bovine Serum Albumin
50% Glycerol
Immunogen
A peptide corresponding to HIF1 alpha

Storage Information

Storage
Store at -20°C only.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use
Guarantee
12 months from date of despatch

More Information

UniProt
Q16665
Entrez Gene
HIF1A
GO Terms
GO:0001666 response to hypoxia
GO:0001837 epithelial to mesenchymal transition
GO:0001938 positive regulation of endothelial cell proliferation
GO:0002248 connective tissue replacement involved in inflammatory response wound healing
GO:0003677 DNA binding
GO:0003705 sequence-specific enhancer binding RNA polymerase II transcription factor activity
GO:0004871 signal transducer activity
GO:0005737 cytoplasm
GO:0005667 transcription factor complex
GO:0005730 nucleolus
GO:0008134 transcription factor binding
GO:0051879 Hsp90 protein binding
GO:0010575 positive regulation vascular endothelial growth factor production
GO:0010634 positive regulation of epithelial cell migration
GO:0046886 positive regulation of hormone biosynthetic process
GO:0030528 transcription regulator activity
GO:0030949 positive regulation of vascular endothelial growth factor receptor signaling pathway
GO:0032364 oxygen homeostasis
GO:0032722 positive regulation of chemokine production
GO:0032909 regulation of transforming growth factor-beta2 production
GO:0032963 collagen metabolic process
GO:0035035 histone acetyltransferase binding
GO:0042789 mRNA transcription from RNA polymerase II promoter
GO:0043193 positive regulation of gene-specific transcription
GO:0043619 regulation of transcription from RNA polymerase II promoter in response to oxidative stress
GO:0045648 positive regulation of erythrocyte differentiation
GO:0045766 positive regulation of angiogenesis
GO:0045821 positive regulation of glycolysis
GO:0045944 positive regulation of transcription from RNA polymerase II promoter
GO:0046982 protein heterodimerization activity
GO:0051000 positive regulation of nitric-oxide synthase activity
GO:0051541 elastin metabolic process
Regulatory
For research purposes only

Applications of HIF1 Alpha antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunohistology - Paraffin 1/500 1/1000
Western Blotting 1/1000 1/2000
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Rabbit IgG (Fc):Biotin STAR121B E WB 1 mg loader
Goat anti Rabbit IgG (Fc):FITC STAR121F F 1 mg loader
Goat anti Rabbit IgG (Fc):HRP STAR121P E WB 1 mg loader
Sheep anti Rabbit IgG:FITC STAR34B C F 1 mg loader
Sheep anti Rabbit IgG:DyLight®488 STAR36D488GA F IF WB 0.1 mg
Sheep anti Rabbit IgG:DyLight®680 STAR36D680GA F IF WB 0.1 mg
Sheep anti Rabbit IgG:Dylight®800 STAR36D800GA F IF WB 0.1 mg
Goat anti Rabbit IgG (H/L):HRP STAR124P C E WB 1 mg loader
Sheep anti Rabbit IgG:RPE STAR35A F 1 ml loader

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