Hexokinase 2 antibody | OTI4C5

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Anti Hexokinase 2 Antibody, clone OTI4C5 (PrecisionAb Monoclonal Antibody) thumbnail image 1

Anti Hexokinase 2 Antibody, clone OTI4C5 (PrecisionAb Monoclonal Antibody) thumbnail image 2

Anti Hexokinase 2 Antibody, clone OTI4C5 (PrecisionAb Monoclonal Antibody) thumbnail image 3

Anti Hexokinase 2 Antibody, clone OTI4C5 (PrecisionAb Monoclonal Antibody) thumbnail image 4

Anti Hexokinase 2 Antibody, clone OTI4C5 (PrecisionAb Monoclonal Antibody) gallery image 1 — Western blot analysis of whole cell lysates probed with Mouse anti Human hexokinase 2 antibody, clone OTI4C5 (**VMA00174**) followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000, STAR207P) and visualized on the ChemiDoc MP with 32 second exposure. Arrow points to hexokinase 2 (molecular weight 102 kDa).

Anti Hexokinase 2 Antibody, clone OTI4C5 (PrecisionAb Monoclonal Antibody) gallery image 2 — Western blot analysis of Hexokinase 2 knockout 293T and wild type 293T (WT) whole cell lysates probed with Mouse anti Hexokinase 2 antibody (**VMA00174**) followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000 STAR207P) and visualized on the ChemiDoc MP with a 3 second exposure. Tubulin was detected with hFAB rhodamine anti-tubulin primary antibody (12004166) and visualized on the ChemiDoc MP with a 30 second exposure.

Anti Hexokinase 2 Antibody, clone OTI4C5 (PrecisionAb Monoclonal Antibody) gallery image 3 — Normalized signal intensity of Hexokinase 2 from CRISPR knockout and wild type lysates. Signal intensity was normalized to the tubulin signal.

Anti Hexokinase 2 Antibody, clone OTI4C5 (PrecisionAb Monoclonal Antibody) gallery image 4 — Immunoprecipitation of Hexokinase 2 from K562 whole cell lysate. The Input lane is 10% input; the Hexokinase 2-IP lane is the immunoprecipitation with the Hexokinase 2 antibody (2.5 µg, **VMA00174**); the IgG-IP lane is the immunoprecipitation with Mouse monoclonal IgG(2.5 µg, MCA928). Western blot analysis was performed using Hexokinase 2 antibody (1/2,000, **VMA00174**) followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000, STAR207P) and visualized on the ChemiDoc MP. Arrow points to Hexokinase 2.

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WB IP

Mouse anti Hexokinase 2

Product Type: PrecisionAb Monoclonal
Clone: OTI4C5
Isotype: IgG1
Format: Purified
Specificity: Hexokinase 2

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VMA00174 Datasheet Datasheet SDS SDS	IP WB	100 µl		Log in	Get Quote
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Mouse anti Human hexokinase 2 antibody, clone OTI4C5 recognizes hexokinase 2 (HK2), also known as HK II, hexokinase type II and muscle form hexokinase.

Hexokinases phosphorylate glucose to produce glucose-6-phosphate, the first step in most glucose metabolism pathways. Hexokinase 2 is the predominant form found in skeletal muscle. It localizes to the outer membrane of mitochondria. Expression of HK2 is insulin-responsive and HKII expression in rat suggest that it is involved in the increased rate of glycolysis seen in rapidly growing cancer cells (provided by RefSeq, Apr 2009).

Mouse anti Human hexokinase 2 antibody detects a band of 102 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Target Species: Human
Western Blotting: Anti hexokinase 2 detects a band of approximately 102 kDa in K562 cell lysates.
Species Cross-Reactivity: Target Species Cross Reactivity

Rat

Mouse; N.B. Antibody reactivity and working conditions may vary between species.
Product Form: Purified IgG - liquid
Preparation: Mouse monoclonal antibody purified by affinity chromatography from ascites.
Buffer Solution: Phosphate buffered saline.
Preservative Stabilisers: 0.09% Sodium Azide (NaN₃)
1% Bovine Serum Albumin
50% Glycerol
Immunogen: Full length recombinant human hexokinase 2 (NP_000180) produced in HEK293T cell.
Approx. Protein Concentrations: IgG concentration 0.5 mg/ml.
Regulatory: For research purposes only.
Guarantee: 12 months from date of despatch.
Acknowledgements: PrecisionAb is a trademark of Bio-Rad Laboratories.

Target Species	Cross Reactivity
Rat
Mouse

Store undiluted at -20°C, avoiding repeated freeze thaw cycles.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Immunoprecipitation
Western Blotting		1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Description	Product Code	Applications	Pack Size	Your Price
Goat anti Mouse IgG (H/L):HRP	STAR207P	WB	2 ml	Log in	Get Quote
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Description	Goat anti Mouse IgG (H/L):HRP

UniProt: P52789
Entrez Gene: HK2
GO Terms: GO:0004340 glucokinase activity; GO:0005829 cytosol; GO:0005524 ATP binding; GO:0005741 mitochondrial outer membrane; GO:0006096 glycolysis; GO:0008637 apoptotic mitochondrial changes; GO:0015758 glucose transport; GO:0055085 transmembrane transport