Hexokinase 2 antibody | OTI4C5
KO Validated
IP-MS Validated
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WB IP ResetMouse anti Hexokinase 2
- Product Type
- PrecisionAb Monoclonal
- Clone
- OTI4C5
- Isotype
- IgG1
- Format
- Purified
- Specificity
- Hexokinase 2
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| Mouse anti Human hexokinase 2 antibody, clone OTI4C5 recognizes hexokinase 2 (HK2), also known as HK II, hexokinase type II and muscle form hexokinase. Hexokinases phosphorylate glucose to produce glucose-6-phosphate, the first step in most glucose metabolism pathways. Hexokinase 2 is the predominant form found in skeletal muscle. It localizes to the outer membrane of mitochondria. Expression of HK2 is insulin-responsive and HKII expression in rat suggest that it is involved in the increased rate of glycolysis seen in rapidly growing cancer cells (provided by RefSeq, Apr 2009). Mouse anti Human hexokinase 2 antibody detects a band of 102 kDa. The antibody has been extensively validated for western blotting using whole cell lysates. |
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- Target Species
- Human
- Western Blotting
- Anti hexokinase 2 detects a band of approximately 102 kDa in K562 cell lysates.
- Species Cross-Reactivity
-
Target Species Cross Reactivity Rat Mouse - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Preparation
- Mouse monoclonal antibody purified by affinity chromatography from ascites.
- Buffer Solution
- Phosphate buffered saline.
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
50% Glycerol - Immunogen
- Full length recombinant human hexokinase 2 (NP_000180) produced in HEK293T cell.
- Approx. Protein Concentrations
- IgG concentration 0.5 mg/ml.
- Regulatory
- For research purposes only.
- Guarantee
- 12 months from date of despatch.
- Acknowledgements
- PrecisionAb is a trademark of Bio-Rad Laboratories.
Store undiluted at -20°C, avoiding repeated freeze thaw cycles.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Immunoprecipitation |  |
||
| Western Blotting | |
1/1000 |
The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Goat anti Mouse IgG (H/L):HRP | STAR207P | WB | 2 ml |
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| List Price | Your Price | ||||||
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| Description | Goat anti Mouse IgG (H/L):HRP | ||||||
- UniProt
- P52789
- Entrez Gene
- HK2
- GO Terms
- GO:0004340 glucokinase activity
- GO:0005829 cytosol
- GO:0005524 ATP binding
- GO:0005741 mitochondrial outer membrane
- GO:0006096 glycolysis
- GO:0008637 apoptotic mitochondrial changes
- GO:0015758 glucose transport
- GO:0055085 transmembrane transport
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"
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