Hexokinase 2 antibody | OTI4C5
Mouse anti Hexokinase 2
Hexokinase 2 antibody recognizes hexokinase 2, also known as HK2 or hexokinase type II. This antibody has been extensively validated for western blotting using whole cell lysates.
- Product Type
- PrecisionAb™ Monoclonal
- Clone
- OTI4C5
- Isotype
- IgG1
- Format
- Purified
| Product Code | Applications | Datasheet | MSDS | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|---|
| VMA00174 | WB | 100 µl |
Mouse anti Human hexokinase 2 antibody recognizes hexokinase 2 (HK2), also known as HK II, hexokinase type II and muscle form hexokinase.
Hexokinases phosphorylate glucose to produce glucose-6-phosphate, the first step in most glucose metabolism pathways. Hexokinase 2 is the predominant form found in skeletal muscle. It localizes to the outer membrane of mitochondria. Expression of HK2 is insulin-responsive, and studies in rat suggest that it is involved in the increased rate of glycolysis seen in rapidly growing cancer cells (provided by RefSeq, Apr 2009).
Mouse anti Human hexokinase 2 antibody detects a band of 102 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
Hexokinases phosphorylate glucose to produce glucose-6-phosphate, the first step in most glucose metabolism pathways. Hexokinase 2 is the predominant form found in skeletal muscle. It localizes to the outer membrane of mitochondria. Expression of HK2 is insulin-responsive, and studies in rat suggest that it is involved in the increased rate of glycolysis seen in rapidly growing cancer cells (provided by RefSeq, Apr 2009).
Mouse anti Human hexokinase 2 antibody detects a band of 102 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
Product Details
- Target Species
- Human
- Western Blotting
- Anti hexokinase 2 detects a band of approximately 102 kDa in K562 cell lysates.
- Species Cross-Reactivity
-
Target Species Cross Reactivity Rat Mouse - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Preparation
- Mouse monoclonal antibody purified by affinity chromatography from ascites.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
50% Glycerol - Immunogen
- Full length recombinant human hexokinase 2 (NP_000180) produced in HEK293T cell.
- Instructions For Use
- Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
Storage Information
- Storage
- Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
- Guarantee
- As supplied, 12 months from date of despatch.
More Information
- UniProt
- P52789 Related reagents
- Entrez Gene
- HK2 Related reagents
- GO Terms
- GO:0004340 glucokinase activity
- GO:0005829 cytosol
- GO:0005524 ATP binding
- GO:0005741 mitochondrial outer membrane
- GO:0006096 glycolysis
- GO:0008637 apoptotic mitochondrial changes
- GO:0015758 glucose transport
- GO:0055085 transmembrane transport
- Acknowledgements
- PrecisionAb™ is a trademark of Bio-Rad Laboratories.
- Regulatory
- For research purposes only
Applications of Hexokinase 2 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Western Blotting | 1/1000 |
PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
Copyright © 2019 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Goat anti Mouse IgG (H/L):HRP | STAR207P | 2 ml | WB |
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