Hexokinase 2 antibody | OTI4C5

100% Secure


Mouse anti Hexokinase 2

Product Type
PrecisionAb™ Monoclonal
Clone
OTI4C5
Isotype
IgG1
Format
Purified
Product Code Applications Pack Size List Price Quantity
VMA00174
Datasheet
WB
MSDS
100 µl loader

Mouse anti Human hexokinase 2 antibody recognizes hexokinase 2 (HK2), also known as HK II, hexokinase type II and muscle form hexokinase.

Hexokinases phosphorylate glucose to produce glucose-6-phosphate, the first step in most glucose metabolism pathways. Hexokinase 2 is the predominant form found in skeletal muscle. It localizes to the outer membrane of mitochondria. Expression of HK2 is insulin-responsive, and studies in rat suggest that it is involved in the increased rate of glycolysis seen in rapidly growing cancer cells (provided by RefSeq, Apr 2009).

Mouse anti Human hexokinase 2 antibody detects a band of 102 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species
Human
Western Blotting
Anti hexokinase 2 detects a band of approximately 102 kDa in K562 cell lysates.
Species Cross-Reactivity
Target SpeciesCross Reactivity
Rat
Mouse
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Mouse monoclonal antibody purified by affinity chromatography from ascites.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
50% Glycerol
Immunogen
Full length recombinant human hexokinase 2 (NP_000180) produced in HEK293T cell.

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Guarantee
As supplied, 12 months from date of despatch.

More Information

UniProt
P52789 Related reagents
Entrez Gene
HK2 Related reagents
GO Terms
GO:0004340 glucokinase activity
GO:0005829 cytosol
GO:0005524 ATP binding
GO:0005741 mitochondrial outer membrane
GO:0006096 glycolysis
GO:0008637 apoptotic mitochondrial changes
GO:0015758 glucose transport
GO:0055085 transmembrane transport
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories.
Regulatory
For research purposes only

Applications of Hexokinase 2 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Mouse IgG (H/L):HRP STAR207P WB 2 ml

Western Blotting without Trial and Error: Use PrecisionAb™ Antibodies

Watch how PrecisionAb Validated Western Blotting Antibodies are defining a new standard of quality that you can trust to deliver superior results and reproducibility in western blotting.