Hemoglobin antibody | AbD05988
Human anti Human hemoglobin antibody, clone AbD05988 recognizes human hemoglobin, a vital oxygen transporting protein that is the major protein constituent of red blood cells.
This antibody recognizes human hemoglobin in Western blot, binding to the 17 kDa sub-unit and multimers thereof. Analysis of binding has demonstrated an affinity of 9nM.
This antibody recognizes human hemoglobin in Western blot, binding to the 17 kDa sub-unit and multimers thereof. Analysis of binding has demonstrated an affinity of 9nM.
Product Details
- Target Species
- Human
- Product Form
- Pack Size: 50 µgA lyophilized, monovalent human recombinant Fab selected from the HuCAL® GOLD phage display library. Expressed in E. coli and purified using purified using NiNTA affinity chromatography. The antibody is tagged with a myc-tag (EQKLISEEDL) and a his-tag (HHHHHH) at the C-terminus of the antibody heavy chain.Pack Size: 0.1 mgA monovalent human recombinant Fab selected from the HuCAL® GOLD phage display library. Expressed in E. coli and purified using purified using NiNTA affinity chromatography. The antibody is tagged with a myc-tag (EQKLISEEDL) and a his-tag (HHHHHH) at the C-terminus of the antibody heavy chain.
- Preparation
- Metal chelate affinity chromatography
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- Pack Size: 50 µgNone presentPack Size: 0.1 mg0.01% Thiomersal
- Immunogen
- Purified human hemoglobin
- Approx. Protein Concentrations
- Pack Size: 50 µgAntibody concentration 0.1 mg/ml following reconstitutionPack Size: 0.1 mgAntibody concentration 0.5 mg/ml
Storage Information
- Storage
- Pack Size: 50 µgStore at -20oC only.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted.Pack Size: 0.1 mgStore at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use. - Shelf Life
- 6 months from date of despatch
More Information
- UniProt
- P69905 Related reagents
- P68871 Related reagents
- Entrez Gene
- HBB Related reagents
- HBA2 Related reagents
- GO Terms
- GO:0022627 cytosolic small ribosomal subunit
- GO:0005515 protein binding
- GO:0005344 oxygen transporter activity
- GO:0005833 hemoglobin complex
- GO:0010942 positive regulation of cell death
- GO:0019825 oxygen binding
- GO:0020037 heme binding
- GO:0042744 hydrogen peroxide catabolic process
- GO:0051291 protein heterooligomerization
- Acknowledgements
- Sold under license of U.S. Patents 6753136, 7785859 and 8273688 and corresponding patents. This antibody was developed by Bio-Rad, Zeppelinstr. 4, 82178 Puchheim, Germany.
His-tag is a registered trademark of EMD Biosciences. - Licensed Use
- For in vitro research purposes only, unless otherwise specified in writing by Bio-Rad.
- Regulatory
- For research purposes only
Applications of Hemoglobin antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA | |||
| Western Blotting | 1/100 Pack Size: 50 µg 1/1000 Pack Size: 0.1 mg |
1/500 Pack Size: 50 µg 1/2500 Pack Size: 0.1 mg |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Technical Advice
- Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual
Copyright © 2018 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Goat anti Human IgG F(ab')2:HRP | 0500-0099 | 0.5 ml | E WB | ||
| Mouse anti c-Myc:HRP | MCA2200P | 0.1 mg | C E P WB* | ||
| Mouse anti Penta Histidine Tag:HRP | MCA5995P | 125 µl | E WB |
