Hemoglobin antibody | AbD05988

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WB ResetHuman anti Human Hemoglobin
- Product Type
- Monoclonal Antibody
- Clone
- AbD05988
- Isotype
- HuCAL Fab monovalent
- Specificity
- Hemoglobin
Human anti Human hemoglobin antibody, clone AbD05988 recognizes human hemoglobin, a vital oxygen transporting protein that is the major protein constituent of red blood cells. This antibody recognizes human hemoglobin in Western blot, binding to the 17 kDa sub-unit and multimers thereof. Analysis of binding has demonstrated an affinity of 9nM. |
- Target Species
- Human
- Product Form
- A monovalent human recombinant Fab selected from the HuCAL GOLD phage display library. Expressed in E. coli and purified using purified using NiNTA affinity chromatography. The antibody is tagged with a myc-tag (EQKLISEEDL) and a his-tag (HHHHHH) at the C-terminus of the antibody heavy chain.
- Preparation
- Metal chelate affinity chromatography
- Source
- E.coli
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.01% Thiomersal
- Immunogen
- Purified human hemoglobin
- Approx. Protein Concentrations
- Antibody concentration 0.5 mg/ml
- Regulatory
- For research purposes only
- Guarantee
- 6 months from date of despatch
- Acknowledgements
- This product and/or its use is covered by claims of U.S. patents, and/or pending U.S. and non-U.S. patent applications owned by or under license to Bio-Rad Laboratories, Inc. See bio-rad.com/en-us/trademarks for details.
His-tag is a registered trademark of EMD Biosciences.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Application Name | Verified | Min Dilution | Max Dilution |
---|---|---|---|
ELISA | ![]() |
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Western Blotting | ![]() |
1/1000 | 1/2500 |
- Technical Advice
- Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual.
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Mouse anti Penta Histidine Tag:HRP | MCA5995P | E WB | 125 µl |
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- RRID
- AB_906036
- UniProt
- P69905
- P68871
- Entrez Gene
- HBB
- HBA2
- GO Terms
- GO:0022627 cytosolic small ribosomal subunit
- GO:0005515 protein binding
- GO:0005344 oxygen transporter activity
- GO:0005833 hemoglobin complex
- GO:0010942 positive regulation of cell death
- GO:0019825 oxygen binding
- GO:0020037 heme binding
- GO:0042744 hydrogen peroxide catabolic process
- GO:0051291 protein heterooligomerization
- View More GO Terms
- Licensed Use
- For in vitro. research purposes only, unless otherwise specified in writing by Bio-Rad.
HCA042


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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"
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