Hemoglobin antibody | AbD05988
This antibody recognizes human hemoglobin in Western blot, binding to the 17 kDa sub-unit and multimers thereof. Analysis of binding has demonstrated an affinity of 9nM.
Product Details
- Target Species
- Human
- Product Form
- A monovalent human recombinant Fab selected from the HuCAL® GOLD phage display library. Expressed in E. coli and purified using purified using NiNTA affinity chromatography. The antibody is tagged with a myc-tag (EQKLISEEDL) and a his-tag (HHHHHH) at the C-terminus of the antibody heavy chain.
- Preparation
- Metal chelate affinity chromatography
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.01% Thiomersal
- Immunogen
- Purified human hemoglobin
- Approx. Protein Concentrations
- Antibody concentration 0.5 mg/ml
Storage Information
- Storage
- Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use. - Guarantee
- 6 months from date of despatch
More Information
- UniProt
- P69905
- P68871
- Entrez Gene
- HBB
- HBA2
- GO Terms
- GO:0022627 cytosolic small ribosomal subunit
- GO:0005515 protein binding
- GO:0005344 oxygen transporter activity
- GO:0005833 hemoglobin complex
- GO:0010942 positive regulation of cell death
- GO:0019825 oxygen binding
- GO:0020037 heme binding
- GO:0042744 hydrogen peroxide catabolic process
- GO:0051291 protein heterooligomerization
- Acknowledgements
- Sold under license of U.S. Patents 6753136, 7785859 and 8273688 and corresponding patents. This antibody was developed by Bio-Rad, Zeppelinstr. 4, 82178 Puchheim, Germany.
His-tag is a registered trademark of EMD Biosciences. - Licensed Use
- For in vitro research purposes only, unless otherwise specified in writing by Bio-Rad.
- Regulatory
- For research purposes only
Applications of Hemoglobin antibody
Application Name | Verified | Min Dilution | Max Dilution |
---|---|---|---|
ELISA | |||
Western Blotting | 1/1000 | 1/2500 |
- Technical Advice
- Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual
Secondary Antibodies Available
Description | Product Code | Applications | Pack Size | List Price | Quantity |
---|---|---|---|---|---|
Goat anti Human IgG F(ab')2:HRP | 0500-0099 | E WB | 0.5 ml |
![]() |
|
Mouse anti c-Myc:HRP | MCA2200P | C E P WB* | 0.1 mg |
![]() |
|
Mouse anti Penta Histidine Tag:HRP | MCA5995P | E WB | 125 µl |
![]() |
Fluorescent Spectraviewer
Watch the Tool Tutorial Video ▸
How to Use the Spectraviewer?
Watch the Tool Tutorial Video ▸
- Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
- Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
- Select the lasers and filters you wish to include
- Select combined or multi-laser view to visualize the spectra