Hemoglobin antibody | AbD05988

Human anti Human Hemoglobin

Product Type
Monoclonal Antibody
Clone
AbD05988
Isotype
HuCAL Fab monovalent
Specificity
Hemoglobin

Product Code Applications Pack Size List Price Your Price Qty
HCA042
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
E WB 0.1 mg loader
List Price Your Price
loader
Search for Batch Specific Datasheets

Human anti Human hemoglobin antibody, clone AbD05988 recognizes human hemoglobin, a vital oxygen transporting protein that is the major protein constituent of red blood cells.

This antibody recognizes human hemoglobin in Western blot, binding to the 17 kDa sub-unit and multimers thereof. Analysis of binding has demonstrated an affinity of 9nM.

Target Species
Human
Product Form
A monovalent human recombinant Fab selected from the HuCAL GOLD phage display library. Expressed in E. coli and purified using purified using NiNTA affinity chromatography. The antibody is tagged with a myc-tag (EQKLISEEDL) and a his-tag (HHHHHH) at the C-terminus of the antibody heavy chain.
Preparation
Metal chelate affinity chromatography.
Buffer Solution
Phosphate buffered saline.
Preservative Stabilisers
0.01% Thiomersal.
Immunogen
Purified human hemoglobin
Approx. Protein Concentrations
Antibody concentration 0.5 mg/ml.
Regulatory
For research purposes only.
Guarantee
6 months from date of despatch.
Acknowledgements
This product and/or its use is covered by claims of U.S. patents, and/or pending U.S. and non-U.S. patent applications owned by or under license to Bio-Rad Laboratories, Inc. See bio-rad.com/en-us/trademarks for details.
His-tag is a registered trademark of EMD Biosciences.

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Western Blotting 1/1000 1/2500
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Technical Advice
Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual.

Description Product Code Applications Pack Size List Price Your Price Quantity
Mouse anti Penta Histidine Tag:HRP MCA5995P E WB 125 µl loader
List Price Your Price
loader
Description Mouse anti Penta Histidine Tag:HRP
Goat anti Human IgG F(ab')2:HRP 0500-0099 E WB 0.5 ml loader
List Price Your Price
loader
Description Goat anti Human IgG F(ab')2:HRP
Mouse anti c-Myc:HRP MCA2200P C E P WB* 0.1 mg loader
List Price Your Price
loader
Description Mouse anti c-Myc:HRP

RRID
AB_906036
UniProt
P69905
P68871
Entrez Gene
HBB
HBA2
GO Terms
GO:0022627 cytosolic small ribosomal subunit
GO:0005515 protein binding
GO:0005344 oxygen transporter activity
GO:0005833 hemoglobin complex
GO:0010942 positive regulation of cell death
GO:0019825 oxygen binding
GO:0020037 heme binding
GO:0042744 hydrogen peroxide catabolic process
GO:0051291 protein heterooligomerization
Licensed Use
For in vitro. research purposes only, unless otherwise specified in writing by Bio-Rad.
View more products with Hemoglobin specificity

Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

View all Anti-Human Products