Heat Shock Protein 40 antibody | OTI1E9

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Anti Heat Shock Protein 40 Antibody, clone OTI1E9 (PrecisionAb Monoclonal Antibody) thumbnail image 1

Anti Heat Shock Protein 40 Antibody, clone OTI1E9 (PrecisionAb Monoclonal Antibody) thumbnail image 2

Anti Heat Shock Protein 40 Antibody, clone OTI1E9 (PrecisionAb Monoclonal Antibody) thumbnail image 3

Anti Heat Shock Protein 40 Antibody, clone OTI1E9 (PrecisionAb Monoclonal Antibody) gallery image 1 — Western blot analysis of whole cell lysates probed with Mouse anti Human heat shock protein 40 antibody (**VMA00131**) followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000, STAR207P) and visualized on the ChemiDoc MP with 8 second exposure. Arrow points to heat shock protein 40 (molecular weight 40 kDa).

Anti Heat Shock Protein 40 Antibody, clone OTI1E9 (PrecisionAb Monoclonal Antibody) gallery image 2 — Western blot analysis of whole cell lysates probed with Mouse anti Human heat shock protein 40 antibody (**VMA00131**) followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000, STAR207P) and visualized on the ChemiDoc MP with 8 second exposure. Arrow points to heat shock protein 40 (molecular weight 40 kDa).

Anti Heat Shock Protein 40 Antibody, clone OTI1E9 (PrecisionAb Monoclonal Antibody) gallery image 3 — Immunoprecipitation of HEAT SHOCK PROTEIN 40 from Jurkat whole cell lysate. The Input lane is 10% input; the HEAT SHOCK PROTEIN 40-IP lane is the immunoprecipitation with the heat shock protein 40 antibody (2.5 μg, **VMA00131**); the IgG-IP lane is the immunoprecipitation with Mouse monoclonal IgG(2.5 μg, MCA929). Western blot analysis was performed using heat shock protein40 antibody (1/1,000, **VMA00131**) followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000, STAR207P) and visualized on the ChemiDoc MP. Arrow points to heat shock protein 40.

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Mouse anti Heat Shock Protein 40

Product Type: PrecisionAb Monoclonal
Clone: OTI1E9
Isotype: IgG2a
Format: Purified
Specificity: Heat Shock Protein 40

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VMA00131 Datasheet Datasheet SDS SDS	IP WB	100 µl		Log in	Get Quote
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Mouse anti Human heat shock protein 40 antibody recognizes heat shock protein 40 (Hsp40), also known as dnaJ homolog subfamily B member 1, dnaJ protein homolog 1, heat shock 40 kDa protein 1, human DnaJ protein 1 and radial spoke 16 homolog B.

Mouse anti Human heat shock protein 40 antibody detects a band of 40 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Target Species: Human
Western Blotting: Anti heat shock protein 40 detects a band of approximately 40 kDa in Jurkat cell lysates.
Product Form: Purified IgG - liquid
Preparation: Mouse monoclonal antibody purified by affinity chromatography from ascites.
Buffer Solution: Phosphate buffered saline.
Preservative Stabilisers: 0.09% Sodium Azide (NaN₃)
1% Bovine Serum Albumin
50% Glycerol
Immunogen: Full length recombinant human heat shock protein 40 (NP_006136) produced in HEK293T cells
Approx. Protein Concentrations: IgG concentration 0.5 mg/ml.
Regulatory: For research purposes only.
Guarantee: 12 months from date of despatch.
Acknowledgements: PrecisionAb is a trademark of Bio-Rad Laboratories.

Store undiluted at -20°C, avoiding repeated freeze thaw cycles.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Immunoprecipitation
Western Blotting		1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Description	Product Code	Applications	Pack Size	Your Price
Goat anti Mouse IgG (H/L):HRP	STAR207P	WB	2 ml	Log in	Get Quote
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Description	Goat anti Mouse IgG (H/L):HRP

UniProt: P25685
Entrez Gene: DNAJB1
GO Terms: GO:0051082 unfolded protein binding; GO:0006986 response to unfolded protein; GO:0005737 cytoplasm; GO:0005730 nucleolus; GO:0031072 heat shock protein binding; GO:0070389 chaperone cofactor-dependent protein refolding