HDGF antibody | AB03/3H6

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Mouse anti HDGF

Product Type
PrecisionAb Monoclonal
Clone
AB03/3H6
Isotype
IgG1
Format
Purified
Product Code Applications Pack Size List Price Quantity
VMA00923
Datasheet
WB
SDS
100 µl loader

Mouse anti HDGF antibody recognizes the Hepatoma-derived growth factor, also known as High mobility group protein 1-like 2.

HDGF is a 27kDa heparin-binding glycoprotein that belongs to the HDGF-related protein family of growth factors. HDGF is ubiquitously expressed and localizes at the nucleus where it acts as a transcription coactivator for lipid metabolism genes (Min et al. 2018) and stimulates cell proliferation (Kishima et al. 2002). HDGF has also been shown to participate in numerous cellular processes such as tissue remodeling, cell migration and angiogenesis (Enomoto et al. 2015) and an increase in its expression has been described as a poor prognosis marker in numerous cancers.

Product Details

Target Species
Human
Western Blotting
Mouse anti HDGF detects a band of approximately 40 kDa in HepG2 cell lysates
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - Liquid
Preparation
Mouse monoclonal antibody affinity purified on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide
Immunogen
E. coli-derived recombinant protein of amino acids 1-240 of human HDGF
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunised BALB/c mice were fused with cells of the mouse SP2/0 myeloma cell line

Storage Information

Storage
Store undiluted at -20°C, avoiding repeated freeze thaw cycles
Guarantee
12 months from date of despatch

More Information

UniProt
P51858
Entrez Gene
HDGF
GO Terms
GO:0000166 nucleotide binding
GO:0003677 DNA binding
GO:0007165 signal transduction
GO:0005615 extracellular space
GO:0005634 nucleus
GO:0005737 cytoplasm
GO:0006350 transcription
GO:0008083 growth factor activity
GO:0008201 heparin binding
GO:0008283 cell proliferation
GO:0045449 regulation of transcription
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories
Regulatory
For research purposes only

Applications of HDGF antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Mouse IgG (H/L):HRP STAR207P WB 2 ml

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