HbA1c antibody | AbD15785_IgG1
Capture antibody, Mouse anti Human hemoglobin, clone 7B12 was coated at 5 μg/ml on a microtiter plate overnight at 4°C. After washing and blocking in PBST + 5% BSA, HbA1c samples (5 μg/ml total hemoglobin concentration) in PBST were added for 1hr at room temperature. Detection was performed by using Human anti HbA1C, clone AbD15783_IgG at a concentration of 2 μg/ml in PBST for 1hr at room temperature. After washing, an HRP-labeled secondary antibody, Goat anti Human IgG F(ab')2:HRP (0500-0099) diluted 1:1000 in HISPEC Assay Diluent (BUF049A) was added. Read out was determined with QuantaBlu using a Tecan Infinite reader. Data is presented as the mean value of quadruplicate experiments.
The data demonstrates the performance of HuCAL generated antibody AbD15785_IgG1 in the presence of abnormal hemoglobin (Hb) samples
1Samples were obtained from heterozygous donors. The values reflect the percentage of abnormal Hb carried by the donor.
*Data from quadruplicate experiments
The data demonstrates the performance of HuCAL generated antibody AbD15785_IgG1 in the presence of abnormal hemoglobin (Hb) samples.
1Samples were obtained from heterozygous donors. The values reflect the mmol/mol of abnormal Hb carried by the donor.
*Data from quadruplicate experiments.
Human anti Human HbA1c
- Product Type
- Monoclonal Antibody
- Clone
- AbD15785_IgG1
- Isotype
- IgG1
- Specificity
- HbA1c
Antibody Quality and Performance Guarantee
| Human anti Human HbA1c antibody, clone AbD15785_IgG1 recognizes HbA1c, hemoglobin glycated at the N-terminal valine of the hemoglobin &beta chain. It does not bind to non-glycated hemoglobin. Besides binding to the normal glycated hemoglobin. Human anti Human HbA1c antibody, clone AbD15785_IgG1 also recognizes the glycated hemoglobin variants HbC, HbE, HbD and to a lesser degree HbS. Elevated HbF is not detected due to the absence of the hemoglobin β chain. The measurement of HbA1c levels is used to identify the average plasma glucose concentration. In diabetes mellitus, higher levels of HbA1c have been associated with cardiovascular disease and other diseases. Levels of more than 6.5% HbA1c (48 mmol/mol) are used as a criterion to diagnose diabetes. This antibody is also available in a Fab format (HCA180 ). |
- Target Species
- Human
- Product Form
- Human IgG1 antibody selected from the HuCAL phage display library and expressed in a human cell line.This antibody is supplied as a liquid.
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A
- Source
- HKB-11
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Immunogen
- The immunogens used in the generation of this antibody were:
A glycated peptide, corresponding to N-terminal 9 amino acids of haemoglobin beta chain coupled to the carrier proteins BSA and TRF
Purified HbA1c - Affinity
- The affinity of this antibody, as a monovalent fab against the immunogen is KD=28 nM as measured by real time, label-free molecular interaction analysis using HbA1c-BSA and HbA1c-TRF conjugates as immobilized antigen.
The affinity of this antibody, as a monovalent fab measured against purified HbA1c is KD=65 nM as measured by real time, label-free molecular interaction analysis using full length HbA1c as immobilized antigen. - Approx. Protein Concentrations
- Total protein concentration 0.5 mg/ml
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
- Acknowledgements
- This product and/or its use is covered by claims of U.S. patents, and/or pending U.S. and non-U.S. patent applications owned by or under license to Bio-Rad Laboratories, Inc. See bio-rad.com/en-us/trademarks for details.
- Licensed Use
- For in vitro research purposes only, unless otherwise specified in writing by Bio-Rad.
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |  |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Technical Advice
- Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual.
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Human anti Human HbA1c | HCA180 | E | 0.1 mg |
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| Description | Human anti Human HbA1c | ||||||
| Human anti Human HbA1c | HCA197 | E | 0.1 mg |
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| Description | Human anti Human HbA1c | ||||||
| Human anti Human HbA1c | HCA218 | E | 0.1 mg |
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| Description | Human anti Human HbA1c | ||||||
HCA217
0213
0213R
160592
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